Method for screening transcription factors related to postharvest softening of grape fruits

A technology for transcription factors and grapes, which can be used in biochemical equipment and methods, genomics, and microbial assay/inspection, etc., and can solve problems such as decreased storage and commercial properties, reduced economic benefits, and affected fruit quality.

Inactive Publication Date: 2022-03-01
HENAN UNIV OF SCI & TECH
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Problems solved by technology

However, the "Kyoho" grape fruit softened rapidly after harvesting. The softened grape

Method used

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  • Method for screening transcription factors related to postharvest softening of grape fruits
  • Method for screening transcription factors related to postharvest softening of grape fruits
  • Method for screening transcription factors related to postharvest softening of grape fruits

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Embodiment Construction

[0028] In the present invention, mainly carry out hypotaurine (Hypotaurine, HT, a kind of active oxygen inhibitor) and hydrogen peroxide (H 2 o 2 ) treatment, through transcriptome sequencing and weighted gene co-expression network analysis (Weightedcorrelation network analysis, WGCNA) analysis, the differentially expressed genes obtained by transcriptome analysis and the change of pectin content were correlated, and the genes closely related to the change of pectin content were screened out. Related differentially expressed gene modules, mining differentially expressed transcription factors in the postharvest softening process of grape fruit. Combining real-time fluorescent quantitative PCR (Quantitative Real-time PCR) and dual-luciferase reporter assay system (Dual-Luciferase Reporter assay system), to further clarify the transcription factors that play a key role in the process of postharvest softening of grape fruit.

[0029] The present invention can quickly obtain the s...

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Abstract

The invention belongs to the technical field of high-throughput sequencing data analysis, and particularly relates to a method for screening transcription factors related to postharvest softening of grape fruits. According to the invention, hypotaurine (HT) and hydrogen peroxide (H2O2) treatment is carried out on harvested 'Kyoho' grapes, through transcriptome sequencing and weighted gene co-expression network analysis (WGCNA), correlation analysis is carried out on differential expression genes obtained through transcriptome analysis and pectin content change, a differential expression gene module closely related to the pectin content change is screened out, and the specific value of the differential expression genes and the specific value of the pectin content change are obtained. And excavating differential expression transcription factors in the softening process of the picked grape fruits. According to the method, the softening character related transcription factors can be effectively screened, and a theoretical reference is provided for researching the softening occurrence mechanism of the picked grape fruits.

Description

technical field [0001] The invention belongs to the technical field of high-throughput sequencing data analysis, and in particular relates to a method for screening transcription factors related to postharvest softening of grape fruit. Background technique [0002] "Kyoho" grape is one of the main varieties planted in my country. It has strong adaptability, disease resistance and high yield; it is easy to manage and reproduce, and it is easy to produce large spikes and large grains, bright color, sweet and sour fruits, and is well received by consumers. And growers love it. However, the "Kyoho" grape fruit softened rapidly after harvesting. The softened grape fruit tasted poor, and the storage and commerciality were reduced. industry development. Therefore, exploring the mechanism of postharvest fruit softening and effective methods to delay softening is an urgent problem to be solved. Contents of the invention [0003] The purpose of the present invention is to provide ...

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Application Information

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IPC IPC(8): C12Q1/6869G16B20/30G16B25/00
CPCC12Q1/6869G16B20/30G16B25/00C12Q2535/122C12Q2539/103
Inventor 刘海楠裴茂松郭大龙余义和韦同路马锦萍晋慧影
Owner HENAN UNIV OF SCI & TECH
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