Method for screening transcription factors related to postharvest softening of grape fruits
A technology for transcription factors and grapes, which can be used in biochemical equipment and methods, genomics, and microbial assay/inspection, etc., and can solve problems such as decreased storage and commercial properties, reduced economic benefits, and affected fruit quality.
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[0028] In the present invention, mainly carry out hypotaurine (Hypotaurine, HT, a kind of active oxygen inhibitor) and hydrogen peroxide (H 2 o 2 ) treatment, through transcriptome sequencing and weighted gene co-expression network analysis (Weightedcorrelation network analysis, WGCNA) analysis, the differentially expressed genes obtained by transcriptome analysis and the change of pectin content were correlated, and the genes closely related to the change of pectin content were screened out. Related differentially expressed gene modules, mining differentially expressed transcription factors in the postharvest softening process of grape fruit. Combining real-time fluorescent quantitative PCR (Quantitative Real-time PCR) and dual-luciferase reporter assay system (Dual-Luciferase Reporter assay system), to further clarify the transcription factors that play a key role in the process of postharvest softening of grape fruit.
[0029] The present invention can quickly obtain the s...
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