Method for extracting resveratrol from moraceae plants

A technology of resveratrol and plants, applied in the direction of chemical instruments and methods, preparation of organic compounds, drug combination, etc., can solve the problems of long cycle, increased production cost, equipment occupancy rate, complex process, etc., and achieve the effect of reducing links

Inactive Publication Date: 2009-12-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are complicated in process and long in cycle, and a variety of chemical and biological reagents need to be

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] Take 1000g of dry mulberry bark and chop it into 1×0.5cm 2 Put the fragments in a stainless steel extraction tank, add 15L of 80% ethanol aqueous solution, heat to a slight boil, keep the slight boil and extract for 1 hour with small stirring. After the extraction is completed, filter with a 200-mesh filter cloth. After the filtrate is properly concentrated, use macroporous adsorption resin (D101 or AB-8, etc.) column chromatography to purify. Elute with an eluent (60% ethanol aqueous solution), collect the eluate, concentrate and dry, and then a resveratrol sample with a content of 50% can be obtained.

example 2

[0029] Take 1000g of dried mulberry leaves and crush them into 1×1cm with a grinder 2 Fragments are placed in a stainless steel extraction tank, add 20L of 60% ethanol aqueous solution, heat to a slight boil, keep the slight boil and extract with small stirring for 1.5h. After the extraction is completed, filter with a 200-mesh filter cloth. After the filtrate is properly concentrated, it first passes through the first macroporous adsorption resin (AB-8 or D101, etc.) chromatography column. After the solution is completely loaded on the column, keep it standing for 20 to 30min, then elute with eluent (70% ethanol aqueous solution), collect eluate, this eluate passes through the second macroporous adsorption resin (DM130 or HPD-500, etc.) After completion, keep standing still for 20 to 30min, then elute with eluent (70% ethanol aqueous solution), collect eluate, after concentrating and drying, just can obtain the resveratrol of higher purity, through HPLC determination, its Th...

example 3

[0031] Take 1000g of dried mulberry peel, put it in a stainless steel extraction tank, add 25L of 75% ethanol aqueous solution, heat to a slight boil, keep the slight boil and stir for 2 hours to extract. After the extraction is completed, filter with a 200-mesh filter cloth. After the filtrate is properly concentrated, it first passes through the first macroporous adsorption resin (AB-8 or D101, etc.) chromatography column. After the solution is completely loaded on the column, keep it standing for 20 to 30min, then elute with eluent (80% ethanol aqueous solution), collect eluate, this eluate passes through the second macroporous adsorption resin (DM130 or HPD-500, etc.) After completion, keep standing still for 20 to 30min, then elute with eluent (80% ethanol aqueous solution), collect eluate, after concentrating, place overnight, just can obtain resveratrol crystal, detect by HPLC, its white The content of veratrol is 98%.

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Abstract

The invention relates to a method for extracting resveratrol, and aims to provide a method for extracting resveratrol from moraceae plants. The method comprises the following steps of raw material selection, crushing, ethanol solvent extraction and purification, wherein in the purification step, the extract solution is put on a polar macroporous adsorbent resin chromatographic column for adsorption, and then eluted by the ethanol solvent, the eluting solution is collected, and the eluting solution undergoes vacuum concentration and drying to obtain the product of the resveratrol. In the process of preparing the resveratrol, only ethanol is used, and the use of chemical reagents is simple; and in the extraction process, the use of materials is also simple, so that the risk due to the use of various chemical reagents is avoided. If the method jointly using two types of macroporous adsorbent resins is adopted, the links in the technological process are greatly reduced, and the operation is easy and easily controlled. If the content of the resveratrol in a sample needs to be improved further, by proper concentration and placing, the crystallization product of the resveratrol, of which the content is 98 percent, can be obtained.

Description

technical field [0001] The invention relates to a method for extracting resveratrol, in particular to a method for extracting resveratrol from Moraceae plants. Background technique [0002] As a natural polyphenolic compound, resveratrol shows a variety of biological activities. Substances with functions such as estrogen regulation are widely used in fields such as medicine and health care. [0003] Resveratrol is currently mainly extracted from certain Chinese medicinal materials, such as Polygonum cuspidatum. At present, resveratrol is extracted from Polygonum cuspidatum, mostly using organic solvents, such as acetone, ethyl acetate, etc., purified with silica gel, and then recrystallized to obtain resveratrol products with high purity. Some methods also add enzyme preparations and alkaline reagents. However, these methods are complicated in process and long in cycle, and various chemical and biological reagents need to be added in the process, and there are too many pr...

Claims

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Application Information

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IPC IPC(8): C07C39/21C07C37/82A61P35/00A61P9/00A61P31/00A61P29/00A61P39/06A61P5/30
Inventor 朱祥瑞占鹏飞
Owner ZHEJIANG UNIV
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