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Sedum alfredii endophyte and application thereof

An endophyte and bacillus technology, applied in the direction of bacteria, microorganisms, microorganism-based methods, etc., can solve the problems of slow growth, weak adaptability, small biomass, etc., to increase the biomass, improve the survival rate, and improve the biomass. Effect

Active Publication Date: 2015-03-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Sedum sedum has some advantages in terms of growth rate and biomass compared with most heavy metal hyperaccumulator plants, its efficiency in remediating soil heavy metal pollution is still limited by slow growth, small biomass, weak adaptability and the availability of heavy metals in the soil. Therefore, how to promote the growth of Sedum sedum, stress resistance, increase biomass and the ability to absorb, transport and accumulate heavy metals in soil is of great significance for the practical application of Sedum sedum to repair heavy metal-contaminated soil

Method used

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  • Sedum alfredii endophyte and application thereof
  • Sedum alfredii endophyte and application thereof
  • Sedum alfredii endophyte and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 promotes the separation of Sedum sedum bioremediation endophytic bacteria

[0042] The wild plants of Sedum sedum with good growth were collected from the mining area, and the endophyte isolation and screening test was carried out immediately.

[0043]Conventional endophyte isolation methods were used. Remove stale leaves from the whole Sedum sedum plant, then rinse with tap water for 30 minutes, and then wash with distilled water 3 times, each time for 3 minutes; use sterile absorbent paper to absorb the water on the plant surface, and then use sterile scissors to cut the leaves, stems and roots Separate, soak each part in 70% alcohol for 3 minutes, wash 3 times with sterile water, 0.5% HgCl 2 Soak for 1 min, then rinse with sterile water for more than 5 times, each time for 1 min, and apply the sterile water used for the last wash to the LB plate to confirm the sterility of the plant surface.

[0044] Prepare LB solid medium (tryptone 10g, yeast extract...

Embodiment 2

[0046] Example 2 Screening, Purification, Preservation and Identification of Endophytic Bacteria Promoting Sedum sedum Bioremediation

[0047] Take out the stored strains from the refrigerator, inoculate them in LB liquid medium, cultivate overnight at 37°C, dip the inoculation loop into a small amount of bacterial liquid and streak again, pick a single colony in LB liquid medium, and grow to the logarithmic phase at 30°C. A part of this bacterial solution is used for preservation. Add 300 μL sterilized glycerol and 700 μL logarithmic phase bacterial solution to a 1.5mL sterile centrifuge tube, store at -80°C (can be stored for 2 years), and the remaining bacterial solution is tested for key physiological and biochemical indicators. Identification.

[0048] 1. Identification of pathogenicity

[0049] Inoculate the above-mentioned logarithmic phase bacterial solution to fresh damaged leaves (Sedum sedum, tobacco, rapeseed), use sterile water as a control, and no leaf disease c...

Embodiment 3

[0073] Example 3 Effects of endophytes on the growth of Sedum sedum and heavy metal soil remediation

[0074] The composition of the nutrient solution is: K 2 SO 4 , 0.7mmol / L; KCl, 0.1mmol / L; Ca(NO 3 ) 2 4H 2 O, 2mmol / L; MgSO 4 ·7H 2 O, 0.5mmol / L; KH 2 PO 4 , 0.1mmol / L; H 3 BO 3 , 10μmol / L; MnSO 4 ·H 2 O, 0.5 μmol / L; ZnSO 4 ·7H 2 O, 1μmol / L; (NH 4 ) 4 MoO 24 4H 2 O, 0.01 μmol / L; FeSO 4 ·7H 2 O dissolved in boiled 20 μmol L -1 Na 2 ·In EDTA solution, 20μmol / L. Use NaOH or HCl to adjust the nutrient night to pH 5.8)

[0075] (1) Hydroponic environment

[0076] Rapid propagation of Sedum sedum by hydroponics. Cut off the newly born branches of Sedum sedum, each about 10cm long, leaving only 5-8 leaves at the top of the branches, insert them into 0.1 times the hydroponic nutrient solution, the temperature is 23-25°C, and the light conditions are 200-200°C. 300μE / m 2 s, after 3 weeks, the branches grow a little root, then culture with 1 times of the above...

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Abstract

The invention discloses a sedum alfredii endophyte and an application thereof. The endophyte is named as Acinetobacter calcoaceticus Sasm3 with a preservation number of CGMCC NO.7334. The invention also discloses an application of the endophyte in plant-microorganism combined remediation on heavy metal contaminated soil. The application comprises the steps of: inoculating sedum alfredii with the endophyte and planting the sedum alfredii inoculated with the endophyte in the heavy metal contaminated soil to be remedied; and from the second growing season of the sedum alfredii inoculated with the endophyte, re-inoculating the sedum alfredii with the endophyte in each growing season, and harvesting the sedum alfredii. The endophyte can be used for enhancing the resistance of the sedum alfredii to high-concentration heavy metal so as to promote the healthy growth of the sedum alfredii in the environment of high-concentration heavy metal, thereby increasing the biomass of the sedum alfredii and enhancing the remediation efficiency of the sedum alfredii to the heavy metal contaminated soil.

Description

technical field [0001] The invention belongs to the technical field of soil bioremediation, and in particular relates to an endophytic sedum sedum and its application. Background technique [0002] With the rapid development of industry, the modernization of agricultural production and the rapid advancement of urbanization, soil heavy metal pollution is becoming more and more serious. [0003] Heavy metals cannot be degraded by microorganisms, and most heavy metals have the characteristics of poor mobility, long residence time, concealment, and irreversibility in the soil. Once the soil is polluted by heavy metals, it is extremely difficult to reduce it. On the one hand, heavy metals in the soil can affect various metabolic activities such as plant growth and cell division, inhibit the growth of crops and reduce crop yields. On the other hand, heavy metals in soil can enter the food chain through the soil-crop system, posing a huge risk to human health. In the face of doze...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 杨肖娥陈宝潘风山冯英
Owner ZHEJIANG UNIV
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