Method for increasing plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction

Abstract

The invention relates to the field of heavy metal polluted plant extraction, and aims to provide a method for increasing the plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction. The method comprises the following steps: preincubating a hyperaccumulator namely sedum alfredii hance with tap water, selecting seedlings which are 1 to 2cm in root lengths, soakingthe root systems into an endophyte composite bacteria solution for inoculating, and then transplanting the seedlings into a black plastic bucket, wherein a nutrient solution in the black plastic bucket contains cadmium; adding the endophyte composite bacteria solution, placing the seedlings into a plant growth chamber for culturing, keeping carbon dioxide in the plant growth chamber being enriched, changing the nutrient solution once every three days, and keeping the cell concentration; culturing under a carbon dioxide enrichment condition for 1 month, and harvesting plants. By adopting the method, the photosynthesis and biomass are enhanced by a carbon dioxide enrichment technology; meanwhile, the endophyte is applied to promote the adsorption and accumulation of heavy metal cadmium by hyper accumulators, the defects of slow plant growth and small biomass in a plant extracting technology are overcome, the environmental adaptability of the hyperaccumulator sedum alfredii hance is enhanced, and the plant extracting efficiency is increased remarkably.

Description

technical field [0001] The invention relates to the field of heavy metal polluted plant extraction, in particular to a method for improving plant extraction efficiency by using carbon dioxide enrichment technology and endophyte interaction. Background technique [0002] Phytoextraction restoration refers to the use of heavy metal enrichment or hyperaccumulation plants to absorb one or several heavy metals from the environment, and transfer and store them to the aboveground parts. Compared with traditional physical and chemical restoration engineering measures, plant extraction restoration has the advantages of low cost, no damage to the ecological environment, no secondary pollution, etc., and has broad application prospects. There are two prerequisites for the application of phytoextraction and remediation technology. One is that plants must be heavy metal-accumulating or hyper-accumulating plants that can accumulate high concentrations of heavy metal elements. The other is...

AI Technical Summary

Problems solved by technology

However, most of the hyperaccumulative plants discovered so far have small biomass and slow growth rate, which limits the large-scale application of plant extraction and restoration technology. Therefore, it is very urgent to create a new and efficient plant extraction and restoration strengthening technology system.

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