Method for increasing plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction

A carbon dioxide, plant extraction technology, applied in the restoration of polluted soil and other directions, can solve the problems of small biomass, limited widespread application of plant extraction and restoration technology, slow growth rate, etc., to promote absorption and accumulation, and improve plant extraction. Efficiency, solving the effect of slow growth

Active Publication Date: 2018-03-06
ZHEJIANG UNIV
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Problems solved by technology

However, most of the hyperaccumulative plants discovered so far have small biomass and slow growth rate, which limits the large-scale application of plant extr

Method used

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  • Method for increasing plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction
  • Method for increasing plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction
  • Method for increasing plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction

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Embodiment Construction

[0024] The feasibility and application effect of the method of the present invention will be further illustrated below through specific examples.

[0025] (1) The hyperaccumulative plant Sedum sedum was placed in an artificial climate chamber, and pre-cultured with tap water for 1 week to allow it to take root. During the cultivation process, the light cycle was 14 hours, and the day and night temperature was 25 / 20°C. The relative humidity of the phytotron was maintained at 70% during the day and 85% at night.

[0026] (2) In addition, get the fresh stem and root system of the superaccumulative plant Sedum sedum southeast that grows normally in ancient lead-zinc mines and carry out surface sterilization and grinding respectively, then utilize DF-ACC-Cd solid medium to cultivate the grinding liquid respectively; Stretch and purify four kinds of endophytic bacteria: Rhodococcus SaSR1 (Rhodococcus), Buttiauxella SaSR9 (Buttiauxella), Stenotrophomonas SaSS5 (Stenotrophomonas) and ...

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Abstract

The invention relates to the field of heavy metal polluted plant extraction, and aims to provide a method for increasing the plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction. The method comprises the following steps: preincubating a hyperaccumulator namely sedum alfredii hance with tap water, selecting seedlings which are 1 to 2cm in root lengths, soakingthe root systems into an endophyte composite bacteria solution for inoculating, and then transplanting the seedlings into a black plastic bucket, wherein a nutrient solution in the black plastic bucket contains cadmium; adding the endophyte composite bacteria solution, placing the seedlings into a plant growth chamber for culturing, keeping carbon dioxide in the plant growth chamber being enriched, changing the nutrient solution once every three days, and keeping the cell concentration; culturing under a carbon dioxide enrichment condition for 1 month, and harvesting plants. By adopting the method, the photosynthesis and biomass are enhanced by a carbon dioxide enrichment technology; meanwhile, the endophyte is applied to promote the adsorption and accumulation of heavy metal cadmium by hyper accumulators, the defects of slow plant growth and small biomass in a plant extracting technology are overcome, the environmental adaptability of the hyperaccumulator sedum alfredii hance is enhanced, and the plant extracting efficiency is increased remarkably.

Description

technical field [0001] The invention relates to the field of heavy metal polluted plant extraction, in particular to a method for improving plant extraction efficiency by using carbon dioxide enrichment technology and endophyte interaction. Background technique [0002] Phytoextraction restoration refers to the use of heavy metal enrichment or hyperaccumulation plants to absorb one or several heavy metals from the environment, and transfer and store them to the aboveground parts. Compared with traditional physical and chemical restoration engineering measures, plant extraction restoration has the advantages of low cost, no damage to the ecological environment, no secondary pollution, etc., and has broad application prospects. There are two prerequisites for the application of phytoextraction and remediation technology. One is that plants must be heavy metal-accumulating or hyper-accumulating plants that can accumulate high concentrations of heavy metal elements. The other is...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00
Inventor 李廷强吴可人罗继鹏李金星宋宇超
Owner ZHEJIANG UNIV
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