Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber

A cucumber fusarium wilt and fungicide technology is applied in the direction of biocides, plant growth regulators, and biocides, which can solve problems such as high residues, enhanced drug resistance, and increased frequency of pesticide use, achieving broad application prospects and promoting The effect of cucumber growth, good control effect

Inactive Publication Date: 2010-07-07
辽宁省农业科学院植物保护研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main measures to prevent and control the disease are chemical control and grafting. Because some producers have used chemical pesticides for a long time, sprayed pesticides blindly, arbitrarily increased the amount of pesticides used, increased the number of pesticides used at will, and even used highly toxic and high-residue pesticides. Lead to the enhancement of drug resistance of the disease, aggravation of pesticide residues, and endanger the health of consumers
Although grafting can reduce the morbidity, but the labor intensity increases and the production cost increases

Method used

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  • Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber
  • Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber
  • Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Test of Promoting Cucumber Radicle and Plant Growth with Plant Endophytic Agents

[0021] The plant endophytic agent of the present invention is 1.0% of peptone, 0.5% of yeast extract, 0.7% of NaCl, and the rest is water, which is made into mother liquor. , pH value 7-8; 140r / min shaking fermentation culture for 46 hours. Dilute about 1000 times for germination.

[0022] 1. Plant endophytic agent promotes radicle growth

[0023] Disinfect the seeds with 3% sodium hypochlorite for 15 minutes, rinse them repeatedly with clean water, put them in a clean petri dish with filter paper, put about 50 seeds in each dish, treat 3 dishes for each, and add bacterial liquid to each petri dish About 15ml, then add the seeds. Put the petri dish into a constant temperature incubator at 25°C. Before sowing (the length of the radicle is about 9 mm), the radicle was cut off, and 100 grains were randomly taken and weighed. The results are shown in Table 1.

[0024] Table 1 Plant endo...

Embodiment 2

[0032] Experiment on Controlling Cucumber Fusarium Wilt with Plant Endophytic Agent

[0033] The plant endophytic agent of the present invention is 1.0% of peptone, 0.5% of yeast extract, 0.7% of NaCl, and the rest is water, which is made into mother liquor. , pH value 7-8; 140r / min shaking fermentation culture for 46 hours.

[0034] The prepared plant endophytic agent was tested for cucumber wilt. From the test results in Table 3, it can be seen that the plant endophytic agent of the present invention has a better control effect on cucumber wilt. Among them, the plant endophytic agent of the present invention has a good control effect of 1000 times, reaching 65.7%, which is equivalent to 68.6% of 50% carbendazim WP control effect.

[0035] Table 3 Analysis of the effect of plant endophytic agents on the control of cucumber wilt

[0036]

[0037]

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Abstract

The invention relates to an endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber, which is prepared by bacillus subtilis. The technical scheme adopted by the endophytic bacterial agent is as follows: mother solution is prepared by 0.7 to 2.0% of peptone, 0.3 to 1.0% of yeast extract, 0.5 to 1.0% of NaCl and the rest percent of water; then, the mother solution with the pH value of 5 to 9 is cultivated for 24 to 72 hours in oscillating and fermenting manners at the speed of 120 to 200r / min under the fermentation temperature of 20 to 32 DEG C according to the liquid volume of 25 to 55% and the inoculum size of 2 to 10%.

Description

Technical field: [0001] The present invention relates to a Bacillus subtilis (Bacillus subtilis, storage date: September 04, 2008, full name of the depository unit: General Microbiology Center of China Microbial Culture Collection Management Committee, depositary unit abbreviation: CGMCC, deposit number: CGMCC No.2659 ), and make fungicide. Background technique: [0002] Cucumber wilt (Fusarium oxysporum f.sp.cucumerinum) is one of the most serious diseases in cucumber production, and it occurs widely in protected areas. The yield loss caused by cucumber wilt is 10%-30% every year, and it is as high as more than 50% in severe years. At present, the main measures to prevent and control the disease are chemical control and grafting. Because some producers have used chemical pesticides for a long time, sprayed pesticides blindly, arbitrarily increased the amount of pesticides used, increased the number of pesticides used at will, and even used highly toxic and high-residue pes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P3/00C12N1/20C12R1/125
Inventor 苗则彦赵奎华刘长远吕国忠梁春浩王辉陈彦孙柏欣黄玉茜
Owner 辽宁省农业科学院植物保护研究所
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