The application relates to the field of daily chemical flavors, and specifically discloses a natural and refreshing floral and fruity flavor, a preparation method and an application thereof. Natural fresh floral fruity fragrance including methyl dihydrojasmonate, ambroxone, ethyl linalool, limonene, o-tert-butylcyclohexyl acetate, lilial, leaf alcohol, allyl heptanoate, leaf acetate Alcohol esters, phyllyl butyrate, methylturkone, vallutone, privet aldehyde, citral, gamma decanolactone, sweet orange oil, lemon oil, virginia cedarwood oil, and fragrance, modifier, fixative agent; its preparation method is: step 1), mixing evenly; step 2), aging; step 3), removing impurities. The natural and fresh floral and fruity essence of this application can be used in daily chemical products. It has unique top notes of melon, peach, and lemon, middle notes of pineapple and black currant, and base notes of amber, musk, oak moss, and cedar. The front, middle and back notes run through the natural floral fragrance, which makes the essence gain the advantages of rich fragrance layers.