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Endophytic bacillus flexus having salt resistance and application thereof

A Campylobacter, salt-tolerant technology, used in plant growth regulators, plant growth regulators, applications, etc.

Active Publication Date: 2017-08-29
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of wild limonium is currently in sharp decline, and urgent protection is needed
At present, there are few reports on beneficial bacteria promoting the growth of wild limonium and improving its stress resistance under high-salt stress

Method used

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  • Endophytic bacillus flexus having salt resistance and application thereof
  • Endophytic bacillus flexus having salt resistance and application thereof
  • Endophytic bacillus flexus having salt resistance and application thereof

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Experimental program
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Embodiment Construction

[0014] 2) After 48 hours of shaking flask culture at 37°C and 150 rpm, the bacteria were collected by centrifugation at 8000 rpm when the bacteria grew to the logarithmic growth phase;

[0015] 3) After washing the bacteria twice with sterile water, adjust the OD value of the bacteria to 0.5-0.6 with sterile water, and the concentration of the bacteria to be 10 7 -10 8 cfu / mL is the salt-tolerance growth promoter.

[0016] The application of the plant salt-tolerant growth-promoting agent containing PE4941 strain in promoting plant growth under salt stress.

[0017] The application of the plant salt-tolerant growth-promoting agent containing PE4941 strain in promoting the growth of Limonium sinensis under salt stress.

[0018] The method that the salt-tolerant plant growth-promoting agent containing PE4941 bacterial strain is used for the growth-promoting method of plants under salt stress is characterized in that, the bacterium liquid (50ml) containing PE4941 thalline OD val...

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Abstract

The invention discloses an endophytic bacillus flexus having salt resistance PE4941, which is preserved in Guangdong microbe bacterial preservation administration center with a preservation number being GDMCC 60176 on May 10th, 2017; and provides an application of the endophytic bacillus flexus for promoting vegetation growth under salt stress. According to the invention, the separated and screened limonium sinense endophytic bacteria bacillus flexus PE4941 has a plurality of growth-promotion characteristics such as nitrogen fixation and iron carrier generation, the endophytic bacteria can tolerate 8% of NaCl, limonium sinense seedlings can be inoculated by pouring root, a salt damage symptom under salt stress is obviously alleviated, biomass is increased, growth effect is promoted, salt resistance of the plants is increased, and the usage of a chemical fertilizer is reduced.

Description

technical field [0001] The invention belongs to the research field of microbial fertilizer and crop adversity protection, and relates to a plant endogenous Bacillus flexus and its application. Background technique [0002] Soil salinization is one of the main abiotic stress factors that threaten global agricultural production and crop yields. At present, nearly 50% of the irrigated land in the world is threatened by different degrees of salt stress. In my country, there are about 9.9×10 7 hm 2 of land suffers from salinity hazards, and the threatened area is increasing year by year. How to improve and utilize large areas of salinized soil, and how to protect and improve the resistance of important economic crops to salt stress are important scientific and technological issues that need to be resolved urgently in the international and life science fields. At present, the main biological improvement method is to cultivate and plant salt-alkali-tolerant crops, such as plantin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08A01G7/06C12R1/07
CPCA01G7/06A01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 秦盛王田田冯维维邢珂王玉丁鹏蒋继宏
Owner XUZHOU NORMAL UNIVERSITY
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