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Plant-growth-promoting endophytic bacterium having polycyclic aromatic hydrocarbons degrading function and application thereof

A polycyclic aromatic hydrocarbon and degrading bacteria technology, applied in bacteria, microorganisms, microorganisms, etc., can solve problems such as unseen patent research and application reports, and achieve the effect of ensuring the safety of agricultural products, reducing the risk of phenanthrene contamination, and achieving good removal effects.

Active Publication Date: 2015-01-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for persistent organic pollutants such as PAHs, there are still few data reports at home and abroad, and there are no relevant patent research and application reports

Method used

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  • Plant-growth-promoting endophytic bacterium having polycyclic aromatic hydrocarbons degrading function and application thereof
  • Plant-growth-promoting endophytic bacterium having polycyclic aromatic hydrocarbons degrading function and application thereof
  • Plant-growth-promoting endophytic bacterium having polycyclic aromatic hydrocarbons degrading function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Bacterial Strains

[0029] Disinfect the fresh A. argentina plants collected from the sewage outlet of Nanjing Jiangning Yangzi Petrochemical Aromatics Plant: rinse the plants with tap water, rinse them with 75% alcohol in an ultra-clean bench for 3-5 minutes, and then rinse them with sterile water for 3-5 minutes. 4 times, then rinse with 1% sodium hypochlorite solution for 2 to 5 minutes, and finally rinse with sterile water several times. Transfer the surface-sterilized plant samples to LB solid plates, and after incubating them at 30°C for 24 hours, check whether there is bacterial growth on the plates. If no colony grows, it means that the surface of the plant is disinfected successfully.

[0030] Move the fully sterilized plants into a sterile mortar, cut them into pieces with sterilized scissors, add sterile water to grind them thoroughly, take the supernatant and add it to 100mL inorganic salt medium with 5% inoculum, a...

Embodiment 2

[0037] Embodiment 2 Fermentation of restoration bacterial agent

[0038] The process of using the above-mentioned strain Pn2 to produce the repairing bacterial agent is as follows: slant seed—shake bottle seed liquid—seed tank—product (the packaging dosage form is liquid bacterial agent).

[0039] 1) Inoculate the test tube species of Massilia sp. Pn2 with the preservation number CGMCC 1.12927 in the fermentation medium, and cultivate it to the logarithmic phase with shaking;

[0040] 2) Inoculate the above-mentioned cultured strains into the seed tank with an inoculation amount of 5%, and cultivate to the logarithmic phase;

[0041] 3) The seed liquid is connected to the production tank for cultivation according to the inoculum amount of 10%;

[0042] 4) During the cultivation process of seed tanks and production tanks, the ventilation rate of sterile air is 1:1.2, the stirring speed is 200 rpm, the cultivation temperature is 30°C, and the cultivation time of the whole proce...

Embodiment 3

[0045] Example 3 Biodegradation experiment of PAHs by bacterial strain Pn2 in culture medium

[0046] In the inorganic salt culture medium (with embodiment 1), add final concentration and be 150mg L ‐1 The phenanthrene was inoculated into strain Pn2 (CGMCC 1.12927) with a 5% inoculum amount, and the inactivated strain Pn2 was used as a control, and cultured on a constant temperature shaker at 30°C. Take regular samples to detect the strain Pn2 at 150 mg·L ‐1 The growth of phenanthrene as the only carbon source and the degradation of phenanthrene, the results are shown in figure 2 . Strain Pn2 can grow well with phenanthrene as the sole carbon source, and within 3 days to an initial concentration of 150mg·L ‐1 The degradation rate of phenanthrene reaches more than 95%.

[0047] According to the above method, the bacterial strain Pn2 (CGMCC 1.12927) was sequentially inserted into the naphthalene-containing (100 mg·L ‐1 ), acenaphthyl (100mg·L ‐1 ), anthracene (50mg·L ‐1 ...

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Abstract

The invention discloses a plant-growth-promoting endophytic bacterium having a polycyclic aromatic hydrocarbons degrading function and application thereof. The plant-growth-promoting endophytic bacterium having the polycyclic aromatic hydrocarbons degrading function, namely Massilia bacterium Pn2 is preserved in China General Microbiological Culture Collection Center and has the preservation number of CGMCC 1.12927. By virtue of a microbial agent prepared by the strain, the degradation rate of phenanthrene of which the initial concentration is 150mg.L<-1> can reach up to above 95% within 3 days under the condition of laboratory shake-flask culture and meanwhile, naphthalene, acenaphthene, anthracene and pyrene can be degraded in different degrees by virtue of the bacterium, the microbial agent is colonized on the surface of a plant root by virtue of a root dipping treatment and then the plant is planted in a nutrient solution contaminated by virtue of phenanthrene so that the content of phenanthrene within a plant body can be reduced by above 50% within short time, the risk of phenanthrene contamination of the plant body is obviously reduced and the endophytic bacterium has a significant plant growth promoting effect.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental polycyclic aromatic hydrocarbons (PAHs) pollution, and relates to a plant growth-promoting endophytic bacterium with the function of degrading PAHs, a bacterial agent for its production, and an application thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are an important class of persistent organic pollutants commonly found in the soil environment composed of two or more benzene rings, which are highly hydrophobic and difficult to degrade. As hydrophobic organic pollutants, PAHs in soil can be absorbed by plants and accumulate in the body. How to reduce the risk of plant PAHs pollution is one of the current research hotspots in the field of agricultural environment. [0003] Plant endophytic bacteria refer to a type of microorganisms that can colonize in the gaps or cells of healthy plant tissues and establish a harmonious symbiotic relationship with ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01
CPCA62D3/02A62D2101/20B09C1/10C02F3/34C02F2101/327C12N1/205C12R2001/01
Inventor 高彦征刘娟刘爽孙凯
Owner NANJING AGRICULTURAL UNIVERSITY
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