A film-forming bacterium rs2 on the root surface with phenanthrene degradation function and its application

A technology for degrading bacteria and functions, applied in the recovery of bacteria and polluted soil, methods based on microorganisms, etc., can solve the problems of patent research and application that have not yet been reported, and achieve good removal effect, low production cost, and guaranteed safety. Effect

Active Publication Date: 2018-07-13
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there have been no reports on the patent research and application of PAHs degradation by film-forming bacteria on the root surface.

Method used

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  • A film-forming bacterium rs2 on the root surface with phenanthrene degradation function and its application
  • A film-forming bacterium rs2 on the root surface with phenanthrene degradation function and its application
  • A film-forming bacterium rs2 on the root surface with phenanthrene degradation function and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The isolation and identification of embodiment 1 bacterial strain

[0032] Collect the main representative healthy plant samples (Pipetia chinensis, plantain, clamgrass, etc.) near the outfall area of ​​an aromatics plant in Nanjing, Jiangsu Province. Gently remove the soil on the root surface of the collected plant samples with a sterilized brush, then place them in a centrifuge tube filled with 5 mL of sterilized ultrapure water and shake vigorously for 30 seconds to destroy the bacterial biofilm structure on the root surface and free the bacteria out, the obtained bacterial suspension was used as the bacterial community in the root surface biofilm for later use.

[0033] Add the bacterial suspension obtained above to 100 mL of inorganic salt medium at an inoculum size of 5%, and add phenanthrene to 100 mg L ‐1 As the sole carbon source, at 30°C, 180r·min ‐1 Cultivate on a shaking table for 7 days, transfer to the same medium with 5% inoculum amount, after three con...

Embodiment 2

[0041] Embodiment 2 repairs the fermentation of bacterial agent

[0042] The process of using the above-mentioned strain RS2 to produce the restoration bacterial agent is as follows: slant seed—shake bottle seed solution—seed tank—product (the packaging dosage form is liquid bacterial agent).

[0043] 1) Inoculate the test tube species of Sphingobium sp. RS2 with the preservation number CGMCC NO.10982 in the fermentation medium, and vibrate the culture to the logarithmic phase;

[0044] 2) Inoculate the above-mentioned cultured strains into the seed tank with an inoculation amount of 5%, and cultivate to the logarithmic phase;

[0045] 3) The seed liquid is connected to the production tank for cultivation according to the inoculum amount of 10%;

[0046] 4) During the cultivation process of seed tanks and production tanks, the ventilation rate of sterile air is 1:1.2, the stirring speed is 200 rpm, the cultivation temperature is 30°C, and the cultivation time of the whole pro...

Embodiment 3

[0049] Bacterial strain RS2 in the medium of embodiment 3 is to the biodegradation experiment of phenanthrene

[0050] In the inorganic salt culture medium (with embodiment 1), add final concentration and be 100mg L ‐1 The phenanthrene was inoculated into the strain RS2 (CGMCC NO.10982) with a 5% inoculum amount, and the inactivated strain RS2 was inoculated as a control, and cultured on a constant temperature shaker at 30°C. Sampling at regular intervals to detect strain RS2 at 100mg·L ‐1 The growth of phenanthrene as the only carbon source and the degradation of phenanthrene, the results are shown in figure 2 . Strain RS2 can grow well with phenanthrene as the sole carbon source, and can respond to an initial concentration of 100 mg·L within 2 days ‐1 The degradation rate of phenanthrene reaches more than 95%.

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Abstract

The invention discloses a root surface film-forming bacterium RS2 with phenanthrene degradation function and its application. A sphingosine strain RS2 with phenanthrene degrading function was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection on June 16, 2015, with the strain preservation number CGMCC NO.10982. The microbial agent prepared by using this strain can degrade more than 95% of phenanthrene with an initial concentration of 100 mg·L-1 within 2 days under the laboratory shake flask culture conditions. The colonization and film formation of strain RS2 on the root surface can effectively reduce the risk of phenanthrene pollution in plants, and can reduce the phenanthrene content in plant roots by 40% in a short period of time, and the removal effect on the aboveground parts of plants is particularly significant, up to 60% above; at the same time, the accumulation and conductivity of phenanthrene in plants were also significantly reduced.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental polycyclic aromatic hydrocarbons (PAHs) pollution, and relates to a root surface film-forming bacterium RS2 with phenanthrene degradation function and application thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a common type of persistent organic pollutants in the soil environment composed of two or more benzene rings. They have chronic toxicity and "three-induced" effects, strong hydrophobicity, and are difficult to degrade. PAHs in the environment can be absorbed into plants through plant absorption, affect the normal physiological activities of plants at the level of plant organs, tissues, cells, and genes, cause oxidative stress in plants, and even lead to cell death. In addition, PAHs can be transmitted through the food chain and threaten human health. Therefore, how to reduce the risk of plant PAHs pollution is one of the current research hot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/01C02F101/32A62D101/20
Inventor 刘娟高彦征盛月慧
Owner NANJING AGRICULTURAL UNIVERSITY
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