Root surface film forming bacterium RS2 with phenanthrene degradation function and application of root surface film forming bacterium RS2

A technology of degrading bacteria and functions, applied in the direction of bacteria, microorganism-based methods, and restoration of polluted soil, etc., can solve the problems of patent research and application that have not yet been reported, and achieve good removal effect, convenient use and low production cost. Effect

Active Publication Date: 2015-12-30
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no reports on the patent research and application of PAHs degradation by film-forming bacteria on the root surface.

Method used

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  • Root surface film forming bacterium RS2 with phenanthrene degradation function and application of root surface film forming bacterium RS2
  • Root surface film forming bacterium RS2 with phenanthrene degradation function and application of root surface film forming bacterium RS2
  • Root surface film forming bacterium RS2 with phenanthrene degradation function and application of root surface film forming bacterium RS2

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The isolation and identification of embodiment 1 bacterial strain

[0032] Collect the main representative healthy plant samples (Pipetia chinensis, plantain, clamgrass, etc.) near the outfall area of ​​an aromatics plant in Nanjing, Jiangsu Province. Gently remove the soil on the root surface of the collected plant samples with a sterilized brush, then place them in a centrifuge tube filled with 5 mL of sterilized ultrapure water and shake vigorously for 30 seconds to destroy the bacterial biofilm structure on the root surface and free the bacteria out, the obtained bacterial suspension was used as the bacterial community in the root surface biofilm for later use.

[0033] Add the bacterial suspension obtained above to 100 mL of inorganic salt medium at an inoculum size of 5%, and add phenanthrene to 100 mg L ‐1 As the sole carbon source, at 30°C, 180r·min ‐1 Cultivate on a shaking table for 7 days, transfer to the same medium with 5% inoculum amount, after three con...

Embodiment 2

[0041] Embodiment 2 repairs the fermentation of bacterial agent

[0042] The process of using the above-mentioned strain RS2 to produce the restoration bacterial agent is as follows: slant seed—shake bottle seed solution—seed tank—product (the packaging dosage form is liquid bacterial agent).

[0043] 1) inoculate the Sphingobium sp. RS2 test tube species with the preservation number CGMCCNO.10982 in the fermentation medium, and shake and culture until the logarithmic phase;

[0044] 2) Inoculate the above-mentioned cultured strains into the seed tank with an inoculation amount of 5%, and cultivate to the logarithmic phase;

[0045] 3) The seed liquid is connected to the production tank for cultivation according to the inoculum amount of 10%;

[0046] 4) During the cultivation process of seed tanks and production tanks, the ventilation rate of sterile air is 1:1.2, the stirring speed is 200 rpm, the cultivation temperature is 30°C, and the cultivation time of the whole proces...

Embodiment 3

[0049] Bacterial strain RS2 in the medium of embodiment 3 is to the biodegradation experiment of phenanthrene

[0050] In the inorganic salt culture medium (with embodiment 1), add final concentration and be 100mg L ‐1 For the phenanthrene, strain RS2 (CGMCC NO.10982) was inoculated with a 5% inoculum amount, and an inactivated strain RS2 was used as a control, and cultured on a constant temperature shaker at 30°C. Sampling at regular intervals to detect strain RS2 at 100 mg·L ‐1 The growth of phenanthrene as the only carbon source and the degradation of phenanthrene, the results are shown in figure 2 . Strain RS2 can grow well with phenanthrene as the sole carbon source, and can respond to an initial concentration of 100 mg·L within 2 days ‐1 The degradation rate of phenanthrene reaches more than 95%.

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Abstract

The invention discloses a root surface film forming bacterium RS2 with a phenanthrene degradation function and application of the root surface film forming bacterium RS2. The root surface film forming bacterium RS2 with the phenanthrene degradation function is collected in China general microbiological culture collection center in June 16, 2015 and the culture collection number is CGMCC No.10982. The degradation rate of phenanthrene with the initial concentration of 100mg L<-1> can reach more than 95% within 2d on the culture condition of flask shaking of bactericides prepared from the bacterium in a laboratory. Phenanthrene pollution risks of plant bodies can be reduced effectively by root surface colonization and film formation of the bacterium RS2, the phenanthrene content of plant roots can be reduced by 40% in a short time, removal effect on plant overground parts is remarkable, and removal rate can reach more than 60%; meanwhile, the accumulation amount and the conductivity coefficient of the phenanthrene in the plant bodies are reduced remarkably.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental polycyclic aromatic hydrocarbons (PAHs) pollution, and relates to a root surface film-forming bacterium RS2 with phenanthrene degradation function and application thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a common type of persistent organic pollutants in the soil environment composed of two or more benzene rings. They have chronic toxicity and "three-induced" effects, strong hydrophobicity, and are difficult to degrade. PAHs in the environment can be absorbed into plants through plant absorption, affect the normal physiological activities of plants at the level of plant organs, tissues, cells, and genes, cause oxidative stress in plants, and even lead to cell death. In addition, PAHs can be transmitted through the food chain and threaten human health. Therefore, how to reduce the risk of plant PAHs pollution is one of the current research hot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/01C02F101/32A62D101/20
Inventor 刘娟高彦征盛月慧
Owner NANJING AGRICULTURAL UNIVERSITY
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