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Growth promoting rhizobacteria PHE-2 with PAHs degradation capability and application thereof

A rhizosphere growth-promoting bacterium, PHE-2 technology, applied in the direction of application, bacteria, biocide, etc., can solve the problems of lack of bacteria resources, etc., achieve the effects of convenient use, reduce the risk of phenanthrene pollution, and low production cost

Active Publication Date: 2019-08-30
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, due to the lack of corresponding strain resources, the research and application of root surface film-forming bacteria with growth-promoting ability to degrade PAHs have not been reported.

Method used

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  • Growth promoting rhizobacteria PHE-2 with PAHs degradation capability and application thereof
  • Growth promoting rhizobacteria PHE-2 with PAHs degradation capability and application thereof
  • Growth promoting rhizobacteria PHE-2 with PAHs degradation capability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The isolation and identification of embodiment 1 bacterial strain PHE-2

[0032] Collect samples of Aurantia chinensis near the sewage outlet area of ​​an aromatics factory in Nanjing City, Jiangsu Province, gently remove the soil on the root surface of the plant with a sterilized brush, and then place the root in a centrifuge tube filled with 5 mL of sterilized ultrapure water and shake vigorously 30s, to destroy the bacterial biofilm structure on the root surface to free the bacteria, and the obtained bacterial suspension is used as the bacterial community in the root surface biofilm for future use.

[0033] Add the bacterial suspension to 100 mL of inorganic salt medium at an inoculum size of 2%, and add phenanthrene to 100 mg·L -1As the sole carbon source, at 30°C, 180r·min -1 Cultivate on a shaker for 7 days, transfer to the same medium with 1% inoculum, after three consecutive transfers, serially dilute the enrichment solution, take 10 -4 ~10 -7 Each 0.1mL of t...

Embodiment 2

[0040] The fermentation of embodiment 2 functional bacterial agents

[0041] The process of using strain PHE-2 to produce functional bacterial agent is as follows: slant seed—shake bottle seed solution—seed tank—product (the packaging dosage form is liquid bacterial agent).

[0042] (1) Inoculate the Paenibacillus sp. PHE-2 test tube species with the preservation number CGMCCNO.1.12900 in the fermentation medium, and vibrate to the logarithmic phase;

[0043] (2), above-mentioned cultivated bacterial classification is inoculated into seed tank by 5% inoculum amount, is cultivated to logarithmic phase, obtains seed liquid; The seed liquid is inserted into production tank by 10% inoculum amount for cultivation;

[0044] Wherein, the used fermentation medium of the seed tank and the used fermentation medium of the production tank are the same as the fermentation medium of step (1), and the formula is: peptone 10.0g L -1 , Yeast 5.0g·L -1 , NaCl 10.0g·L -1 , pH 7.0-7.4;

[004...

Embodiment 3

[0047] The biodegradation experiment of bacterial strain PHE-2 to phenanthrene and other PAH in the medium of embodiment 3

[0048] In the inorganic salt culture medium (with embodiment 1), adding initial concentration is 100mg L -1 The phenanthrene (or initial concentration of 100mg L -1 Anthracene or an initial concentration of 50mg L -1 The initial concentration of pyrene or 50mg L -1 of ), insert the functional bacterial agent of embodiment 2 with 5% inoculum, set inoculation inactivated PHE-2 as contrast, 30 ℃ constant temperature shaker 180r min -1 Shake culture. Sampling was taken regularly to detect the growth and degradation of the strain PHE-2 when the above-mentioned various PAHs were used as carbon sources.

[0049] Quantity determination of bacterial strain PHE-2 in the culture medium adopts serial dilution coating plate counting, the determination of the residual phenanthrene content in the culture medium is the same as that in Example 1; the determination ...

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Abstract

The invention discloses growth promoting rhizobacteria PHE-2 with PAHs degradation capability. The rhizobacteria is classified and named as Paenibacillus sp., and is preserved in the China General Microbiological Culture Collection Center on August 26th, 2014 with a preservation number of CGMCC No.1.12900. A functional microbial inoculum prepared from the strain PHE-2 has a phenanthrene degradation rate of 95% or above within 5 days under the condition of shake flask culture, wherein the initial concentration of the phenanthrene is 100 mg x L<-1>. Meanwhile, the strain PHE-2 can also rapidly degrade anthracene, pyrene and chrysene. The strain PHE-2 has good root surface film forming ability, has various growth promoting properties, can generate indoleacetic acid and iron carrier, and has phosphorus dissolving ability. The strain PHE-2 can be directly used for reducing the pollution risk of phenanthrene in PAHs-polluted water, soil and plants, and can obviously promote the growth of plants.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental polycyclic aromatic hydrocarbons (PAHs) pollution, and relates to a rhizosphere growth-promoting bacterium (Paenibacillus sp.) PHE-2 with PAHs degradation ability and application thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic aromatic hydrocarbons, PAHs) are compounds composed of two or more benzene ring structures. As a common class of persistent organic pollutants (POPs) in soil, PAHs are chronically toxic, mutagenic, and carcinogenic. , teratogenic "three-cause" effect and biodegradability. Due to the toxicity and harmfulness of PAHs, my country has already included PAHs in the blacklist of pollutants for environmental priority detection. In 2014, the "National Soil Pollution Survey Bulletin" jointly issued by the Ministry of Environmental Protection and the Ministry of Land and Resources pointed out that the point-exceeding rate of polycyclic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00B09C1/10C02F3/34C12R1/01C02F101/32
CPCA01N63/00B09C1/10C02F3/34C02F2101/327C12N1/20C12N1/205C12R2001/01
Inventor 刘娟陈志高高彦征
Owner NANJING AGRICULTURAL UNIVERSITY
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