Microbial group and microbial agent and application thereof

A microbial inoculum and microbial technology, applied in the field of microorganisms, can solve the problems of short duration, poor effect, hurt confidence, etc., and achieve the effect of improving nitrogen fixation effect, increasing total biomass, and promoting vegetative growth.

Active Publication Date: 2021-08-06
GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves how certain types of organisms live inside roots called rhizomes or tubers that support their own life by increasing the efficiency with regards to nitrox fixing within them. It also enhances the ability to promote vegetative growth when applied at specific locations along soil surfaces like rice fields where they were introduced into these areas during irrigations. Overall this results in increased production rates from agricultural land compared to traditional methods such as fertilizers and pesticides.

Problems solved by technology

Technologies aim towards improving the efficiency and reliance of microbe-based technologies like biofermentations and genetic engineering processes through identifying specific populations called phytophagists and fungi that contribute significantly to enhancing soil quality, reducing harmfulness levels, promotes vegetative tissue remodeling, enhancement of yield potentialities, prevention against disease threatening wildlife reserves, increasing tolerancements for nitrogen sources, creativity improvements associated with growing crops, greenhouses, hydroponzones, and natural resources.

Method used

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  • Microbial group and microbial agent and application thereof
  • Microbial group and microbial agent and application thereof
  • Microbial group and microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the monoculture of microorganism

[0054] The resuscitated single-species bacterial liquid or colonies were added to the corresponding culture liquid as seed liquid, and 0.3 mL of seed liquid was added for every 100 mL. Culture in a constant temperature shaking incubator, 28°C, 150-200 rpm, fermentation cycle 24-48h, the concentration of live bacteria in the fermentation broth is not less than 10 8 individual / mL.

[0055] Microscopic examination of nitrifying bacteria and nitrogen-fixing bacteria: Take the cultured nitrifying bacteria liquid and nitrogen-fixing bacteria liquid, fully shake and dilute in ultrapure water, draw 0.3mL and drop it on the slide, and put the slide into the drying box Dried, stained using the Gram staining method, and observed under an oil microscope (see figure 1 and figure 2 ).

[0056] Microscopic examination of Flavobacterium amineoxidans and Azospirillum brasiliensis: Take the cultured liquid of Flavobacterium amineoxi...

Embodiment 2

[0057] Embodiment 2, a kind of microbial bacterial group and microbial bacterial agent

[0058] A microbial bacterial agent includes a microbial bacterial group consisting of nitrifying bacteria and nitrogen-fixing bacteria.

[0059]A preparation method of microbial inoculum, comprising the following steps:

[0060] 1) Add the nitrifying bacteria into the improved liquid nutrient gravy medium according to the mass ratio of 1:1000, place them in a constant temperature shaking shaker for cultivation, set the shaker parameters at 28°C, 150r / min, and after 24-48h, the revived nitrifying bacteria are obtained seed liquid;

[0061] 2) Add nitrogen-fixing bacteria into the improved liquid nitrogen-fixing medium according to the mass ratio of 1:1000, place them in a constant temperature shaking shaker for cultivation, set the shaker parameters at 28°C, 150r / min, and after 24-48h, obtain resurrected nitrogen-fixing bacteria seeds liquid;

[0062] 3) Add the nitrifying bacteria see...

Embodiment 3

[0068] Embodiment 3, a kind of microbial bacterial group and microbial bacterial agent

[0069] A microbial bacterial agent comprises a microbial bacterial group consisting of Flavobacterium oxidans and Azospirillum brasiliensis.

[0070] A preparation method of microbial inoculum, comprising the following steps:

[0071] 1) Add Flavobacterium amineoxidans to the improved liquid nutrient gravy medium according to the mass ratio of 1:1000, place it in a constant temperature shaking shaker for cultivation, set the shaker parameters at 28°C, 150r / min, and after 24-48h, the revived Flavobacterium amine oxide seed solution;

[0072] 2) Add Azospirillum brasiliensis to the improved liquid nitrogen-fixing medium according to the mass ratio of 1:1000, and place it in a constant temperature shaking shaker for cultivation. Set the shaker parameters at 28°C, 150r / min, and after 24-48h, the revived Brazil will be obtained. Azospirillum seed solution;

[0073] 3) Add the seed solution...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a microbial group and a microbial agent and application thereof. The microbial group comprises the following genus, a) nitrifying bacteria and b) nitrogen-fixing bacteria, specifically, the nitrifying bacteria comprise one of the genus xanthobacter, nitrobacter and nitrosomonas, and the nitrogen-fixing bacteria comprise one of the genus Klebsiella, Rhodospirillum, Azospirillum and Bayelinkia. The strains of the microbial group are reasonably and effectively matched, the microbial group can exist in a rhizosphere environment for a long time, the effects of high pesticide effect and lasting efficacy can be achieved, the strains of the microbial group promote and depend on one another, the nitrogen fixation effect of nitrogen-fixing bacteria is greatly improved, meanwhile, invalid nitrogen is changed into effective nitrogen, and the vegetative growth of crops is effectively promoted.

Description

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Claims

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Application Information

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Owner GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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