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Tistrella mobilis bacterial strain, polycyclic aromatic hydrocarbon degradation reagent, soil remediation reagent, and the application of Tistrella mobilis bacterial strain

A polycyclic aromatic hydrocarbon, Rhodospirillum technology, applied in the field of microorganisms to achieve the effect of expanding the scope and repairing soil

Active Publication Date: 2013-09-25
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, many microorganisms capable of degrading PAHs have been reported at home and abroad, but few have been successfully applied to soil remediation. This is mainly related to the properties of PAHs, the degradation ability of microorganisms, and the influence of various environmental factors.

Method used

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  • Tistrella mobilis bacterial strain, polycyclic aromatic hydrocarbon degradation reagent, soil remediation reagent, and the application of Tistrella mobilis bacterial strain
  • Tistrella mobilis bacterial strain, polycyclic aromatic hydrocarbon degradation reagent, soil remediation reagent, and the application of Tistrella mobilis bacterial strain
  • Tistrella mobilis bacterial strain, polycyclic aromatic hydrocarbon degradation reagent, soil remediation reagent, and the application of Tistrella mobilis bacterial strain

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Embodiment

[0031] 1. Isolation, Purification and Identification of Rhodospirillum H1

[0032] A. Culture medium preparation

[0033] 1. beef extract peptone medium: select the commonly used beef extract peptone medium at present;

[0034] ② Inorganic salt medium (MS): Each liter of the above inorganic salt medium (MS) contains K 2 HPO46.0g, KH 2 PO 4 5.5g, NH 4 NO 3 2.0g, Na 2 SO 4 2.0g, KCl2.0g, MgSO4 7H 2 O0.2g, 1.0mL trace metal salt solution, the balance is distilled water, wherein the pH of the above-mentioned inorganic salt medium (MS) is 7.0;

[0035] ③Inorganic salt medium containing PAHs: Prepare 1000 mg / L different PAHs solutions with dimethylformamide (N,N-Dimethylformamide, DMF), including PAHs solutions containing phenanthrene and PAHs solutions containing benzo[a]pyrene , the PAHs solution of pyrene and the PAHs solution of fluoranthene were quantitatively added to the sterilized inorganic salt medium, so that the final concentration was 100 mg / L.

[0036] B. Is...

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Abstract

The invention relates to the technical field of microorganisms, and in particular relates to a Tistrella mobilis bacterial strain. The bacterial strain is H1, and the preservation number thereof is CGMCC No.6869. The invention also provides a bacterial strain biological pure culture which has all identification characteristics of the Tistrella mobilis bacterial strain H1 having the preservation number of CGMCC No.6869. The invention also provides a polycyclic aromatic hydrocarbon degradation reagent, and the polycyclic aromatic hydrocarbon degradation reagent includes the Tistrella mobilis bacterial strain or the biological pure culture. The invention also provides a soil remediation reagent, and the soil remediation agent includes the Tistrella mobilis bacterial strain or the biological pure culture. The Tistrella mobilis bacterial strain provided by the invention can effective degrade polycyclic aromatic hydrocarbons in the environment, and the scope of the microorganisms that can degrade the polycyclic aromatic hydrocarbons is enlarged.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to a new rhodospirilla strain and its application in polycyclic aromatic hydrocarbon degradation reagents and soil restoration reagents. 【Background technique】 [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons PAHs) are hydrocarbons produced when coal, petroleum, wood, tobacco, organic polymer compounds and other organic substances are incompletely burned, and are important environmental and food pollutants. More than 200 kinds of PAHs have been found so far, and quite a few of them are carcinogenic, such as benzo[α]pyrene, benzo[α]anthracene, etc. In the early 1980s, the U.S. Environmental Protection Agency identified 16 unbranched PAHs as priority pollutants in the environment; the International Cancer Research Center (IARC) (1976) listed 94 compounds that are carcinogenic to experimental animals, of which 15 kinds belong to polycyclic aromatic ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/20
Inventor 荆磊毛健邓宗武
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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