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Application of Rhodospirillum rubrum in control of tumorous stem mustard clubroot and tumorous stem mustard clubroot control agent

A technology of Rhodospirillum crimson and Stem mustard, which is applied in the fields of application, bactericide, biocide, etc., to achieve the effects of broadening the application range, good market application prospects, and increasing the vegetable yield

Active Publication Date: 2016-08-24
YANGTZE NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Rhodospirillum rubrum is a kind of photosynthetic bacteria. In the prior art, Rhodospirillum rubrum is mostly used in the treatment of organic wastewater, and there is no related research on using Rhodospirillum rubrum to prevent and treat clubroot.

Method used

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  • Application of Rhodospirillum rubrum in control of tumorous stem mustard clubroot and tumorous stem mustard clubroot control agent
  • Application of Rhodospirillum rubrum in control of tumorous stem mustard clubroot and tumorous stem mustard clubroot control agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 Rhodopseudomonas palustris bacterial strain and cultivation

[0017] The strain number of Rhodospirillum rubrum: 1.5005 was purchased from China General Microorganism Culture Collection and Management Center. The formula of the 0989 culture medium (provided by the China Common Microorganism Culture Collection and Management Center) adopted for the cultivation of Rhodospirillum rubrum is as follows:

[0018] 0989 medium (composition and final concentration): yeast extract 2.00g / L, trypticase peptone 2.00g / L, potassium dihydrogen phosphate 0.30g / L, dipotassium hydrogen phosphate 0.3g / L, ammonium sulfate 0.3g / L, sodium chloride 0.60g / L, magnesium sulfate heptahydrate 0.13mg / L, calcium chloride dihydrate 2mg / L, sodium acetate 2.5g / L, sodium formate 2.5g / L, trace element solution 10ml / L, vitamin Solution 10ml / L, sodium bicarbonate 5g / L, L-cysteine ​​0.5g / L, sodium sulfide nonahydrate 0.5g / L, resazurin 1mg / L.

[0019] Among them, the composition and final conc...

Embodiment 2

[0021] Embodiment 2 inoculation method

[0022] The plots with severe clubroot disease were selected, and Yongan Xiaoye, a cultivar commonly used in Fuling District, was used as the experimental variety. When transplanting, the roots of the tuber mustard seedlings were cut off, and 100 mL of Rhodospirillum rubrum was suspended. Liquid (1×10 9 cfu mL -1 ) to 500 mL with water and mix well (final concentration 2×10 8 cfu / mL), 60 root-cut seedlings were soaked for 30 min, and the parallel operation experiment was repeated three times. With clean water as the blank control, the plots were randomly arranged, and the management was the same as that of the general field. During the harvest period, the field disease rate, disease index and number of grown vegetables were investigated in the treatment area and the control area, and the control effect of the strains in the treatment area was measured.

Embodiment 3

[0023] Embodiment 3 disease statistics

[0024] 1. Statistical method:

[0025] The severity of clubroot mustard disease is graded as 5 grades, and the standards are (Gao Mingquan, 2012):

[0026] Grade 0: Healthy plants.

[0027] Grade 1: Lateral root disease, small and few tumors, white in color, no or slight effect on plant growth, yield loss of 0-5%.

[0028] Grade 2: Tumors are mainly in the middle of the main root, and there are many upper and lower lateral roots, or large and numerous tumors on the lateral roots, which are white or beige, and have certain effects on plant growth, but there are no obvious symptoms in the aerial part, and the yield loss is 6-20%.

[0029] Grade 3: The main root tumor is large and black, the surface is rough and cracked, there are a few lateral roots, the growth of the aboveground part is hindered, the central leaves turn dark green, and they wilt slightly in the sun, and the yield loss is 21-35%.

[0030] Grade 4: The main root tumor c...

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Abstract

The invention provides an application of Rhodospirillum rubrum in control of tumorous stem mustard clubroot and a tumorous stem mustard clubroot control agent. In specific application, Rhodospirillum rubrum is prepared into 1 to 2*10<8> cfu / mL bacterial suspension with water; before being transplanted into a field for culture, a tumorous stem mustard seedling is subjected to root cutting treatment; the root-cut tumorous stem mustard seedling is subjected to root soaking treatment for 30 to 60 min according to a manner that every 100mL bacterial suspension is used to treat 10 to 20 tumorous stem mustard seedlings. The tumorous stem mustard clubroot control agent comprises the Rhodospirillum rubrum which is taken as a main active component for controlling the tumorous stem mustard clubroot. Proved by experimental results, when the seedlings are treated by applying the Rhodospirillum rubrum and by adopting the specific application method provided by the invention, the finished-product rate when the tumorous stem mustard is harvested is improved by 12.5 percent, the disease index of the clubroot is reduced by 25.4 percent, and the control rate to the clubroot reaches 33 percent.

Description

technical field [0001] The invention belongs to the technical field of clubroot prevention and control, and in particular relates to the application of Rhodospirillum crimson in the prevention and treatment of mustard clubroot and an agent for preventing and treating clubroot. Background technique [0002] "Fuling mustard" has become a characteristic agriculture in Chongqing, my country. The planting area and output of mustard in Fuling District rank first in the country. Mustard mustard clubroot was discovered in Fuling in 1994, and now it has become a major disease of mustard (Gao Mingquan, Peng Hongjiang, Wang Xu, etc. Determination of damage and yield loss of mustard clubroot in Fuling. Plant Protection, 2002, 28(6): 31-33; Xiao Chonggang, Guo Xianghua, Han Haibo, et al. Identification and main characteristics of mustard clubroot pathogen in Fuling. Journal of Southwest Agricultural University, 2002, 24(4): 539-541), continuous cropping years of mustard in Fuling area U...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00
CPCA01N63/00
Inventor 王殿东潘丽梅田美子王旭祎李昌满秦明一杨银平刘欢杨霞
Owner YANGTZE NORMAL UNIVERSITY
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