Immunocontraceptive synthetic peptide and immunocontraceptive chimeric peptide and application thereof

A technology of immune contraception and chimeric peptide, applied in the field of immunology, can solve problems such as autoimmune diseases, endocrine balance and sexual dysfunction, poor birth control effect, etc., and achieve a strong anti-fertility effect

Inactive Publication Date: 2012-06-27
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above contraceptive methods have disadvantages such as endocrine balance and sexual dysfunction, autoimmune diseases, poor birth control effect, etc.

Method used

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  • Immunocontraceptive synthetic peptide and immunocontraceptive chimeric peptide and application thereof
  • Immunocontraceptive synthetic peptide and immunocontraceptive chimeric peptide and application thereof
  • Immunocontraceptive synthetic peptide and immunocontraceptive chimeric peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Effects of ESP protein and cross-reactive antigen of Ureaplasma urealyticum on fertility

[0063] 1. Materials

[0064] 1.1 Experimental animals and specimens

[0065] 6-month-old (weight about 2.5kg) female New Zealand white rabbits and BALB / c mice (8-12W) were purchased from the Department of Animal Science, Shanghai Jiaotong University School of Medicine, from the Experimental Animal Center of the Chinese Academy of Sciences, and normal human semen samples were obtained from Shanghai Jiaotong University Medicine Provided by the human sperm bank clinic of Renji Hospital Affiliated to the hospital.

[0066] 1.2 Main reagents

[0067] Oligonucleotide sequences and peptides were synthesized by Shanghai Sangon Biotechnology Company, and keyhole limpet hemocyanin (KLH) was purchased from Shanghai Sangon Biotechnology Company. Plasmid pTSA18 was purchased from Shanghai Institute of Family Planning Science and Technology.

[0068] 2.2.1.3 Experimental instruments Mini Pr...

Embodiment 2

[0139] Identification of cross-reactive antigens between UU474 protein of UU and human sperm protein IZUMO

[0140] 1. Experimental animals and materials:

[0141] Sexually mature male and female BALB / c mice (provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences) with reproductive history were used for both in vivo and in vitro reproductive experiments in mice.

[0142] Male New Zealand white rabbits aged 6 months (about 2.5 kg in weight) were purchased from the Animal Science Department of Shanghai Jiao Tong University School of Medicine.

[0143] 2. Main reagents:

[0144] UU liquid medium and solid medium (Shanghai Tongji Hospital Ministry of Railways Key Laboratory of STD); restriction enzymes BamH I, Hind III, EcoR I, T4 DNA ligase, gel recovery DNA kit, reverse transcription kit, La Taq enzyme (Dalian Bao Biological Company); pre-stained low molecular weight standard protein Marker, low molecular weight standard protein Marker (Fermentas); Agoros...

Embodiment 3

[0242] Study on the function of chimeric peptide immunocontraceptive vaccine

[0243] 1. Materials Synthesis Peptide CP1 was synthesized by Shanghai Sangon Biotechnology Company.

[0244] Complete Freund's adjuvant and incomplete Freund's adjuvant, horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG, FITC-labeled goat anti-rabbit IgG, Anti-His monoclonal antibody (Sigma company) PVDF membrane ( Millipore), ECL bioluminescent chromogen (Millipore).

[0245] 6-month-old (weight about 2.5kg) female New Zealand white rabbits and mature BALB / c mice (8-12W) were purchased from the Experimental Animal Center of the Chinese Academy of Sciences from the Department of Animal Science, Shanghai Jiaotong University School of Medicine.

[0246] Second, the method

[0247] 1. Design and synthesis of chimeric peptides

[0248] The cross-reactive antigens in the sperm protein Izumo, tNASP, and ESP are ATLTK, IERLT, and TGGFT, respectively, and the three short peptides hESP 103-112 (TGG...

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Abstract

The invention relates to immunocontraceptive synthetic peptide and immunocontraceptive chimeric peptide, wherein the amino acid sequence of the immunocontraceptive chimeric peptide is shown as SEQ ID:1 in a sequence table, the amino acid sequence of the immunocontraceptive synthetic peptide hESP103-107 is shown as SEQ ID:2 in the sequence table, and the amino acid sequence of the immunocontraceptive synthetic peptide hIzumo216-220 is shown as SEQ ID:3 in the sequence table. The invention also provides the application of the chimeric peptide, the synthetic peptide hESP103-107 or the synthetic peptide hIzumo216-220 as immunocontraceptive vaccines observed in contraception. The invention researches the chimeric peptide comprising sperm protein hESP, hIzumo and tNASP as well as the cross reacting antigens of UU (Ureaplasma Urealyticum) for the first time, and immunized mice can obtains stronger and reversible antifertility effect.

Description

【Technical field】 [0001] The invention relates to the field of immunology, in particular to a synthetic peptide and chimeric peptide for immunocontraception and applications thereof. 【Background technique】 [0002] The principle of contraception is to use scientific methods to prevent and interfere with certain links in the normal conception process, so as to avoid pregnancy and prevent birth. The contraceptive method that adopts at present is a lot, and the prior art that is used for immunocontraception both at home and abroad has: 1. anti-gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) vaccine; 2. anti-ovum zona pellucida (ZP) protein vaccine; ③ anti-human chorionic gonadotropin (hCG) vaccine; ④ anti-sperm surface antigen vaccine. The above contraceptive methods have shortcomings such as endocrine balance and sexual dysfunction, autoimmune diseases, and poor birth control effects. 【Content of invention】 [0003] Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C07K7/06C07K7/08A61K39/00A61P15/18
Inventor 徐晨吕正梅王旻
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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