17 results about "Necrospermia" patented technology

The invention provides an application of methylsulfonylmethane in preparation of a product for treating asthenospermia. The research of the invention finds that the sulfhydrylation modification level of ATP synthetase can be effectively changed by using methylsulfonylmethane as an active component, so that the intracellular energy metabolism state of sperms is regulated and controlled, and the motility of the sperms is further changed.

The application discloses an immunogen for preparing testis-specific glyceraldehyde-3-phosphate dehydrogenase lysine malonylation site-specific antibody, and belongs to the technical field of biotechnology.The immunogen is a malonylation modified polypeptide, and the amino acid sequence is IIPASTGAAKAVT, corresponding to the 278th-290th amino acid of a human GAPDHS protein, wherein a lysine at the 287th position (the 10th position in the sequence) is subjected to malonylation modification.A specific antibody designed according to the immunogen has the characteristics of high titer and high specificity. By screening a human sperm sample by using the antibody, it is found that the antibody can not only specifically recognize the GAPDHS with the lysine at the 287th position subjected to malonylation modification in vitro and in human sperm, but also is closely related to sperm forward movement and asthenozoospermia. The application has great significance in the field of preparation of reagents for precision diagnosis and treatment of idiopathic male infertility.

The invention discloses a method for improving the quality of male sperms. Belongs to the technical field of biological medicine. It is found that the sperm quality of male mice can be remarkably improved by long-term in-vivo application of D-mannose. The method is mainly embodied in that kinematics parameters of total sperm motility rate and forward motility rate are remarkably increased, and the effect is independent of gonad development improvement; if the sperm mitochondrial membrane potential rises, the energy metabolism function is improved; the sperm acrosome integrity is improved, and the sperm quality is integrally optimized. The results show that D-mannose, as a safe natural monosaccharide, can significantly improve the quality of male sperms in an in-vivo intervention manner, and provides a new method and approach for treatment and intervention of male infertility, especially asthenozoospermia.

The invention relates to application of PDCD5 in preparation of a diagnostic agent or a therapeutic agent for asthenospermia. The invention also discloses a method for constructing an asthenospermia mouse model or inhibiting fibroblast cilia generation by knocking out or knocking down the PDCD5. The application shows that PDCD5 participates in sperm generation, and asthenozoospermia can be caused if the PDCD5 has function deletion mutation in testis. Therefore, the gene locus can be used as a target spot for asthenospermia diagnosis and medication guidance. According to the method disclosed by the invention, the dysfunction of the site is supplemented by the PDCD5 RNA wrapped by the testis injection liposome, so that the asthenospermia caused by the mutation is rescued, the sperm quantity is increased, the sperm malformation rate is reduced, and a new path is provided for the treatment of the asthenospermia.

The present application provides use of compounds represented by formula (I) and formula (II) in the preparation of drugs for improving male reproductive ability. In the present application, different oligoasthenospermia models are constructed, and the improvement effects of the compounds represented by formula (I) and formula (II) on male reproductive ability impairment are verified by means of sperm count analysis, sperm motility analysis, mRNA expression level analysis of inflammatory and antioxidant genes, testicular histopathological damage analysis, etc. The experimental results show that the compounds represented by formula (I) and formula (II) increase the sperm count in mice with oligoasthenospermia, improve the sperm motility of the mice with oligoasthenospermia, ameliorate testicular histopathological damage in the mice, suppress the expression of inflammatory factors, and increase the expression of antioxidant genes, demonstrating great potential in the treatment of oligoasthenospermia.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

The invention discloses a traditional Chinese medicine composition for treating infertility, which is prepared from the following raw materials: radix rehmanniae recen, radix paeoniae alba, turtle shell, cortex moutan, peach kernel, ginseng, radix glehniae, medlar, oyster, rhizoma cyperi, radix curcumae, astragalus membranaceus, angelica sinensis, rhizoma anemarrhenae, golden cypress, lotus seed, rhizoma atractylodis macrocephalae, pericarpium citri reticulatae, chicken's gizzard-membrane, radix bupleuri, tetrapanax papyriferus, donkey-hide gelatin, eucommia ulmoides and deer glue. Spleen tonifying, blood nourishing, phlegm reducing and dampness clearing; the effects of soothing liver-qi stagnation, promoting qi and activating blood are achieved; qi is benefited, blood is nourished, and Zhongzhou is promoted to regenerate Qi and blood; promoting blood circulation to remove blood stasis and dredging Chong and Ren qi; the traditional Chinese medicine composition has the effects of clearing heat, promoting diuresis, eliminating dampness and reducing phlegm, can achieve sufficient qi and blood of liver and kidney and smooth Chong and Ren channels, has a good treatment effect on male and female infertility caused by various reasons, and is particularly suitable for female infertility caused by kidney deficiency, weakness, fallopian tube blockage, cold uterus, kidney deficiency type ovulation disorder, obesity, stasis, weakness, blood deficiency and the like; and the traditional Chinese medicine composition is also suitable for male infertility caused by cold sperm, rare sperm, oligospermia and necrospermia due to kidney deficiency.

This invention relates to the fields of biomedical diagnostic reagent manufacturing and reproductive medicine technology, and particularly to a detection system and method for assessing sperm motility in patients with asthenospermia. This invention establishes a complete, accurate, and efficient molecular-level sperm quality evaluation system by integrating specific fluorescent labeling, high-resolution confocal imaging, and automated computer analysis software. This system not only overcomes the limitations of existing technologies but also further expands its functional scope by integrating an optical microscopy analysis module. It possesses good clinical applicability and scalability, providing a powerful tool for the etiological diagnosis, efficacy evaluation, and scientific research of male infertility, and has significant clinical application value and market prospects.

The invention discloses a construction method and application of a sperm-specific hOdf2-GFP transgenic mouse model, and relates to the technical field of reproductive biology and transgenic animal model construction. According to the construction method disclosed by the invention, based on a piggyBac transposon expression vector system, a DNA (Deoxyribose Nucleic Acid) sequence containing a coding human source Odf2 is effectively connected with a Protamine1 promoter. The transgenic positive mouse constructed by the method can specifically express human Odf2, has a dynamic change from sperm to zygote to eight cell stages, realizes early continuous visual tracking of embryos, has genetic stability, keeps expression and phenotype consistency across generations, and has a good application prospect. The method can be applied to the fields of environmental poison evaluation, asthenospermia mechanisms, drug screening and the like.

The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and application thereof in preparation of a medicine for improving and / or treating male infertility. The traditional Chinese medicine composition is prepared from Fructus Malli, Fructus Equiseti, Rhiocarpae, Radix Rehmanniae Preparatae, Rubus chingii Hu, Fructus Carpesii, Rhizoma Polygonati, Morus, Fructus Lycii, Semen Marsdeniae, Fructus Schisandrae Chinensis, Semen Plantaginis, Fructus Rosae Laevigatae, Radix Glycyrrhizae Preparatae. The traditional Chinese medicine composition is supplemented with multiple medicinal materials on the basis of a traditional prescription, forms a comprehensive conditioning scheme considering multiple viscera and multiple causes, a multi-dimensional syndrome differentiation and treatment strategy, and is beneficial to the treatment purpose of treating both the symptoms and the root cause, can more effectively cope with the multiple pathological changes of oligospermia and asthenospermia of male infertility, and improve the overall treatment effect. The traditional Chinese medicine composition is applied in cooperation with an assisted reproductive technology, and the effect is remarkable.

This invention discloses an in vivo sperm processing device, control method, and application based on surface acoustic wave (SAW) devices. The non-invasive in vivo sperm processing method based on SAW devices offers significant advantages: it avoids the trauma risks of surgical and acupuncture treatments and solves the problem of unstable effects from hormone therapy and antioxidant therapy. By precisely controlling SAW parameters, this invention significantly improves sperm motility. It is simple to operate, low in cost, and highly safe, and applicable to various types of oligospermia and asthenospermia, providing a highly efficient, non-invasive, and widely applicable new solution for the research and treatment of oligospermia and asthenospermia.

The invention belongs to the technical field of traditional Chinese medicine compositions, and discloses a composition for promoting sperm quality improvement and resisting aging and a preparation method and application thereof, the composition is composed of sun-dried ginseng, fructus alpiniae oxyphyllae, pericarpium citri reticulatae, nutmeg, gardenia, raspberry, dark plum, pawpaw and deer blood freeze-dried powder; the dosage forms of the composition comprise decoction, powder, superfine powder, pills and the like. The composition is applied to preparation of a medicine for treating and / or relieving oligospermia and asthenozoospermia; the traditional Chinese medicine composition disclosed by the invention runs through ancient and modern prescription medicines, the preparation process is mature and controllable, and a novel traditional Chinese medicine choice which is safe, effective and clear in mechanism is provided for clinical treatment of asthenospermia and related anti-aging.

The invention belongs to the technical field of compositions, and provides a medicinal and edible composition for conditioning male oligospermia and asthenospermia and a preparation method of the medicinal and edible composition. The medicinal and edible composition is prepared from the following raw materials: oyster peptide powder, fructus lycii, rhizoma polygonati, radix ginseng, maca, concentrated pomegranate juice and concentrated mulberry juice. The preparation method comprises the following steps: (1) weighing rhizoma polygonati, ginseng, fructus lycii and maca, mixing, crushing and sieving to obtain mixed powder; (2) extracting the mixed powder to obtain a mixed filtrate; concentrating to obtain a second concentrated solution, sterilizing and storing; and (3) dissolving the oyster peptide powder in water, adding the concentrated pomegranate juice, the concentrated mulberry juice and the second concentrated solution, mixing, and filling into a container. The composition with homology of medicine and food has the advantages that the used raw materials are few and fine, the compatibility effect is strong, and the composition has a remarkable spermatogenic function on mice with deficiency of kidney-yang and has an application prospect of improving the symptoms of oligospermia and asthenozoospermia of men; the flavor is good. The preparation method is good in effective component extraction effect and simple to operate, and industrial large-scale production can be realized.

The application provides application of pyridoxamine in sperm of oligospermia, preferably and / or in a freezing and resuscitation operation, pyridoxamine is added in a sperm operation liquid or a semen freezing liquid, and the following shortcomings of patients with oligospermia are effectively improved: (1) the ratio of sperm forward motility; (2) the ratio of sperm forward motility after semen freezing and resuscitation; (3) the sperm DNA fragmentation rate after semen freezing and resuscitation; (4) the sperm acrosome enzyme content after semen freezing and resuscitation; in summary, pyridoxamine can be added in the sperm operation liquid or the semen freezing liquid as a natural semen protective agent, the sperm selection treatment and the freezing and resuscitation effect of patients with oligospermia are obviously improved, and the application is suitable for application of patients with oligospermia in assisted reproductive treatment and fertility preservation.

The invention discloses an immunogen for preparing a testis specific glyceraldehyde-3-phosphate dehydrogenase lysine malonylation site specific antibody, and belongs to the technical field of biology. The immunogen is malonylation modified polypeptide, the amino acid sequence of the immunogen is IPASTGAAKAVT, the immunogen corresponds to the 278-290 amino acid of human GAPDHS protein, and the 287 site (the 10 site of the sequence) lysine is subjected to malonylation modification. A specific antibody designed according to the immunogen has the characteristics of high titer and high specificity. A human sperm sample is screened by using the antibody, and the antibody is found to be capable of specifically recognizing the 287th lysine malonylation modified GAPDHS in vitro and in human sperms, and the GAPDHS-K287mal level in the sperms is also closely related to the forward movement of the sperms and asthenozoospermia. The invention has great significance in the field of preparation of precise diagnosis and treatment reagents for idiopathic male infertility.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to a detection system and method for evaluating the sperm motility function of asthenozoospermia. By integrating specific fluorescence labeling, high-resolution confocal imaging and computer automatic analysis software, a complete, accurate and efficient molecular level sperm quality evaluation system is established. The system not only overcomes the limitation of the prior art, but also further expands the functional range of the system by integrating the optical microscope analysis module, has good clinical applicability and expansibility, provides a powerful tool for etiological diagnosis, curative effect evaluation and scientific research of male infertility, and has a wide application prospect. Important clinical application values and market prospects are realized.

The invention provides application of a compound shown in a formula (I) in preparation of a medicine for improving male reproductive capacity. According to the application, a nano micro-plastic and plasticizer induced Kunming mouse environment pollution type oligospermia and asthenospermia model, a streptozotocin induced Kunming mouse diabetes type oligospermia and asthenospermia model and a sodium valproate induced Kunming mouse anti-epileptic drug type oligospermia and asthenospermia model are respectively constructed; the improvement effect of the compound shown in the formula (I) on male reproductive capacity damage is verified through sperm counting analysis, sperm motility analysis, inflammation and antioxidant gene mRNA expression level analysis, testis tissue pathological damage analysis and the like, and experimental results show that the compound shown in the formula (I) increases the sperm number of mice suffering from oligospermia and asthenospermia, and has a good application prospect. According to the application, the sperm motility of mice with oligospermia and asthenozoospermia is improved, the tissue pathological damage of the testis is improved, the expression of inflammatory factors is inhibited, the expression of antioxidant genes is increased, and the application has great potential in the aspect of treating oligospermia and asthenozoospermia.