38 results about "Sperm Numbers" patented technology

Abstract of the Disclosure The present invention provides a method of producing a mammal through artificial insemination and is directed, in particular embodiments, to low spermatozoa numbers for insemination and the production of a mammal through the use of hysteroscopic insemination techniques. The present invention is particularly directed to embodiments potentially regarding fresh or preserved sperm, treated or processed sperm, sperm inserted under a surface in the vicinity of the uterotubal junction, hysteroscopic compatible media for the establishment of the insemination sample, hysteroscopic compatible volume for insemination, epididymal use of the hysteroscopic technique, bubble or froth insemination utilized in hysteroscopic insemination, and for sorted and frozen sperm utilized in hysteroscopic insemination. The disclosed embodiments may be directed at a mammal species, particularly equids, bovids, and swine, as well as animals produced in accordance with any of the disclosed embodiments of the present invention.

The invention discloses a health-care food for males and a preparation method thereof. The health-care food is prepared according to a traditional Chinese medicine theory and the respective health-care efficacies and characteristics of maca, cistanche and liquorice; maca has the efficacies of resisting fatigues, improving sexual functions, increasing the number of sperms, improving the quality of the sperms, reducing benign prostatic hyperplasia, enhancing fertility and the like; traditional Chinese medicine cistanche has the efficacies of benefiting essence and blood, tonifying the kidney and reinforcing yang as well as lubricating intestines and smoothing bowels and can have health-care effects on kidney deficiency and impotence, spermatorrhea and premature ejaculation, coldness and pain of a waist and knees and the like; liquorice is a tonifying Chinese herbal medicine and can coordinate drug properties as well as tonify spleen and benefit qi. By virtue of a nanotechnology, the bioavailability of bioactive molecules in maca, cistanche and liquorice is improved; by virtue of fine processing, maca, cistanche and liquorice are prepared into the health-care food for males; the health-care food is safe and effective, nontoxic and harmless, and has synergistic effects on improvement of sexual function, enhancement of fertility, regulation of internal secretion, reduction of prostate diseases and improvement of the immunologic function of an organism.

The invention discloses a solution for sperm detection. The solution for sperm detection of the invention comprises hyaluronic acid and L-carnitine, and can be used for sperm-cervical mucus penetration tests. Thereinto, the hyaluronic acid is used as a substitute of human cervical mucus, and L-carnitine can protect sperm motility. Based on conditions that patients with oligozoospermia / asthenospermia have less sperm numbers and low sperm motility, the invention is further improved, and a sperm detection solution for patients with oligozoospermia / asthenospermia is prepared. The sperm detection solution for patients with oligozoospermia / asthenospermia has high sensitivity, and a high concentration of L-carnitine, and the L-carnitine can increase the oxidative damage resistance of sperms and protect sperm motility.

The invention relates to a medicine for treating male oligozoospermia and improving semen quality. Various natural edible plant medicines such as Rhizoma Polygonati, Fructus Lycii, Cordyceps militaris (L.) Link., Fructus Rubi, Fructus Mori, Fructus Hippophae, Semen Coicis, Radix Ginseng, and Concha Ostreae (calcined) serve as raw materials, are combined suitably, and jointly have effects of invigorating the spleen and replenishing Qi, and nourishing the kidney and replenishing essence, Lepidium Meyenii contains macaenes and macamides which are used as active substances has effects of promoting sexual function, increasing the number of sperms and improving activity of the sperms, selenium yeast, aminoacid, L-carnitine tartrate, calcium carbonate and fructose can strengthen and protect energy metabolism of the sperms, taurine and vitamin effectively remove reactive oxide species and protect activity of the sperms, and folacin can promote maturity of the sperms and improve functions of the sperms. The medicine is reasonable in composition, and the synergistic effect of various components is utilized, so that the medicine is remarkable and lasting in treatment effect, safe and reliable and convenient to take orally, and can effectively promote spermatogenesis, improve survival rate of the sperms and improve activity of the sperms.

The invention discloses a fry cultivating method for loaches. The fry cultivating method specifically comprises the steps of parent loach selection, parent loach cultivation, artificial spawning induction, artificial insemination, fertilized egg hatch, loach fry management and loach fry treatment. According to the fry cultivating method for the loaches, spawning induction is performed with multiple kinds of oxytocin, and the induced spawning rate reaches 100% by performing back muscle injection with two needles; seminal fluid and spawns of the loaches are acquired through an extrusion method, the female loach number is larger than the male loach number to enable the sperm number to be equivalent to the spawn number, hatching is monitored regularly, the fertilization rate reaches 87.4%-89.3%, and the hatching rate reaches 96.3%-97.5%; loach fries are cultured by stages after breaking out of membranes, the feed feeding amount and the culture density are strictly controlled, and the loach fry survival rate is increased and reaches 86.7%-87.8%; a pond is sterilized and disinfected with traditional Chinese medicine, the traditional Chinese medicine can be naturally decomposed and cannot be retained in loach bodies, and therefore eating is safe and healthy; parent loaches are sold immediately or prepared into dried loaches after being extruded, and therefore economic loss cannot be caused to farmers.

The invention discloses a boar feed. The boar feed contains corns, bean pulp, fish meal, wheat germ, extruded soybeans, calcium hydrophosphate, mountain flour, salt, 50% choline chloride, a micro-ecological preparation, lysine, methionine, threonine, and 0.5-1 part of a boar premix. Per kilogram of the boar premix contains 3500-12000IU of vitamin A, 750-4500IU of vitamin D, 20-100IU of vitamin E, 4-35 mg of copper, 50-250 mg of iron, 40-150 mg of zinc, 16-80 mg of manganese, 0.2-0.8 g of L-carnitine, 5-15 g of omega 3 fish oil, 0.1-0.3 g of selenium yeast, and 0.5-2 g of medium-chain fatty acid MCT. VA, VE, carnitine, omega 3 fish oil and medium-chain fatty acid MCT are added into the boar feed disclosed by the invention; furthermore, the micro-ecological preparation in the invention is used in a matched manner; therefore, the boar sperm density and the total sperm number are increased; the sperm deformation rate of breeding boars is obviously reduced; and thus, the breeding performance of boars is greatly improved.

The invention relates to the technical field of breeding, in particular to a wasp breeding method. The wasp breeding method comprises the following steps: (1) cultivating a queen bee; (2) feeding male bees; (3) breeding; (4) overwintering. According to the wasp breeding method, female bees are bred, feed is changed before mating, food materials and traditional Chinese medicines capable of promoting secretion of female hormone are added, the trace elements in the feed are supplemented, sufficient nutrition preparation is provided for mating, the resistance of the queen bee is effectively guaranteed, the survival rate of newly born bee eggs is effectively increased and reaches 98.1 percent, and the survival rate of young eggs after 7 days reaches 97.5 percent; feed is changed for the male bees before mating, the secretion of male hormone is effectively promoted, the sperm number of the male bees is increased, the sperm quality of the male bees is improved, the insemination rate is effectively increased during mating, guarantee is provided for increasing the survival rate of the young eggs, and the sperm number of single male bee reaches 0.95 [mu]L.

The invention discloses a method for 3D culturing testis cells of bostrychus sinensis to generate sperms. The method for 3D culturing the testis cells of the bostrychus sinensis to generate the spermscomprises the following steps that testes of bostrychus sinensis milters are taken out, cleaned, sterilized and cut into pieces, testis fragments are placed in digestive juice, digestion is carried out on a constant-temperature horizontal shaking table, and a testis cell suspension is prepared; incompletely digestive tissues or large cell clusters are removed through cell mesh screen filtering, filtered cells are subjected to low-speed centrifugation through Percoll gradient, and the testis cells of the bostrychus sinensis after low-speed centrifugal purification are collected; bostrychus sinensis sperm culture mediums and protein additives are added for resuspension, and inoculation is carried out in a 3D cell culture dish for culturing; in the process of 3D culturing, immunofluorescenceis used for detecting germ cells; one half of fresh sperm culture mediums are replaced every 2-5 days, the number of the sperms is counted, and eosin Y dyeing detects the survival rate of the cultured sperms; and the bostrychus sinensis sperms generated by 3D culturing and ripe eggs of the bostrychus sinensis are subjected to in vitro fertilization, and the fertilization rate of an embryo is counted.

The invention relates to special boar compound feed and a preparation method thereof, and belongs to the field of feed compositions and feed processing. The compound feed mainly comprises, by weight, 48.5-57.5 parts of corns, 14-18 parts of bean pulp, 0.5-0.9 part of mountain flour, 5-13 parts of wheat bran, 0.1-0.3 part of choline chloride, 1-5 parts of soybean hulls, 1-2 parts of soybean oil, 1-3 parts of rice bran meal, 1-3 parts of steamed fish meal, 1 part of compound premix, 1-3 parts of alfalfa meal, 2-5 parts of expanded flaxseeds, 0.2-0.5 part of lysine, 0.3-0.7 parts of taurine, 0.15-0.2 part of baking soda, 0.01-0.02 part of L-threonine, 0.03-0.05 part of mycotoxin adsorbents, 0.02-0.03 part of Danquine 1 and 0.5-1 part of glucose. According to the special boar compound feed, sperm number of boars can be increased, sperm activity and sperm density of the boars are improved, sperm aberration rate is reduced, and fertilization rate is increased.

The invention provides a molecular genetic marker related to effective boar sperm count. The molecular genetic marker related to effective boar sperm count is a large-effect molecular genetic marker influential on effective boar sperm count per sperm collection which is successfully screened by performing association analysis on effective boar sperm count and whole-genome molecular genetic markers. The molecular genetic marker, ALGA0123896, is identified as a molecular genetic marker influential on effective boar sperm count. Being used for marking effective sperm counts of different genotypes of boars, ALGA0123896 shows very significant difference. The screened molecular genetic marker influential on effective boar sperm count, namely the ALGA0123896, belongs to intron sequence of NC 010444 gene, and is a T>C mutation at the site. The molecular genetic marker can be detected and applied for breeding on basis of boar semen yield, namely to select and retain homozygous boars with high effective sperm count per sperm collection; and then, continuous selecting and retaining are carried out so as to have effective sperm count in boars effectively improved. Thus, breeding progress of boars with high-quality sperm yield is sped up.

The invention discloses a traditional Chinese medicament for treating hyposexuality of a reserve boar. The traditional Chinese medicament comprises the following raw materials: 12 g of codonopsis pilosula, 9 g of astragalus, 12 g of atractylodes, 9 g of Chinese thorowax root, 12 g of oyster, 9 g of eucommia ulmoides, 12 g of desert cistanche, 9 g of medlar, 9 g of cuscuta chinensis, 9 g of cinnamon, 20 g of dried rehmannia, 30 g of cornus officinalis, 15 g of rhizoma alismatis, 20 g of tuckahoe, 15 g of cassia twig, 30 g of fructus psoraleae, and 15 g of schisandra chinensis. The raw materials are decocted into juice and are administered orally. The traditional Chinese medicament for treating the hyposexuality of the reserve boar disclosed by the invention can tonify kidney and strengthen spleen, has a very good effect on the reserve boar with a thin body and kidney deficiency, and has an obvious effect of improving the concentration and the sperm number of the semen of the reserve boar.

The invention discloses a feed for a breeding pig. The feed for the breeding pig consists of the following raw materials: 57 percent of yellow corn, 5 percent of soybean, 8 percent of soya bean meal, 2 percent of salvinia natans powder, 10 percent of silkworm chrysalis, 3.5 percent of fish meal, 5 percent of meat and bone meal, 0.5 percent of eggshell meal, 0.3 percent of salt, 0.3 percent of calcium carbonate, 0.1 percent of calcium hydrophosphate, 0.2 percent of a vitamin additive, 0.05 percent of ferrous sulfate, 0.03 percent of zinc sulfate, 0.01 percent of lysine, 0.01 percent of methionine, 1 percent of astragalus, 1 percent of oyster, 1 percent of eucommia ulmoides, 1 percent of semen cuscutae, 1 percent of cinnamon, 1 percent of cornus officinalis, 1 percent of rhizoma alismatis, and 1 percent of cassia twig. The feed for the breeding pig disclosed by the invention can meet the requirements on protein and vitamins of the breeding pig, can tonify kidney and spleen, and has a very good effect on a thin breeding pig; meanwhile, because of the adoption of traditional Chinese medicinal components, the feed for the breeding pig disclosed by the invention has an obvious effect of improving the concentration and the sperm number of the semen of a boar.

The invention provides a multifunctional sperm quality analysis system. The multifunctional sperm quality analysis system comprises sperm routine computer-aided sperm analysis (CASA) and image analysis, the CASA is used for analyzing a sample video recorded through a CCD camera or video inputted into the local of an analysis host to calculate the quantity, the concentration, the vitality and the motion trail of sperms of the sample; the image analysis is used for analyzing sperm functions and comprises sperm DNA fragment detection, sperm nucleoprotein detection and sperm morphological detection, and pictures taken through the CCD camera or pictures inputted to the local of the analysis host are analyzed. The problems that existing seminal fluid analysis operation is tedious, human factorsare uncontrollable, and the inspection cost is high are solved.

The invention belongs to the technical field of biological medicine. The invention particularly relates to application of an SGLT2 inhibitor in preparation of a medicine for preventing and treating male reproductive dysfunction. Research results show that dapagliflozin treatment can increase testis weight and testis / body weight ratio of db / db mice, and can significantly improve sperm quality abnormality, sperm quantity abnormality and sperm movement abnormality of the db / db mice. According to the application, the SGLT2 inhibitor is used for preventing or treating male reproductive dysfunction for the first time, an ideal treatment effect is achieved, a new prevention and treatment choice is provided for treating male sterility in clinical application, especially male sterility caused by diabetes or hyperglycemia, and the application has extremely high application value and social benefits.

The invention provides application of geniposide in preparation of a medicine for treating asthenospermia. Experimental results show that the geniposide can significantly improve the sperm quantity and activity and reduce the sperm malformation rate, can significantly inhibit endoplasmic reticulum stress inside testis, increase mitochondrial membrane potential, regulate expression of related proteins in testis tissues, and improve excessive apoptosis of spermatogenic cells.

The invention belongs to the technical field of biomedicines. The invention particularly relates to application of canagliflozin and analogs thereof in preparation of a medicine for preventing and treating male reproductive dysfunction. Research results show that spermatogenic cells at all levels in seminiferous tubules of db / db mice treated by the canagliflozin reappear and are regularly arranged, meanwhile, a small amount of sperms exist in the tubules, and the canagliflozin treatment can be used for significantly improving abnormal sperm quality, abnormal sperm number and abnormal sperm movement of the db / db mice. The canagliflozin and analogs thereof are used for preventing or treating the male reproductive dysfunction for the first time, an ideal treatment effect is achieved, a new prevention and treatment choice is provided for clinical application and treatment of male sterility, especially male sterility caused by diabetes or hyperglycemia, and extremely high application values and social benefits are achieved.

The invention provides a novel application of plant fruit pulp. The invention relates to application of plant fruit pulp in testis function protection, application of plant fruit pulp in prevention and / or treatment of oligospermia and asthenospermia, application of plant fruit pulp in protection of male fertility and application of plant fruit pulp in prevention and / or treatment of testis tissue lesion, testosterone level decline, testis function decline, sperm number reduction, asthenospermia and asthenospermia caused by metabolic disorder. The sperm motility rate is reduced; the sperm morphology is abnormal. The plant fruit pulp can effectively prevent and treat symptoms such as testicular tissue lesion, testosterone level decline, testicular hypofunction, sperm number reduction, sperm motility rate reduction and sperm morphology abnormality caused by metabolic disorder. The application provides a basis for protecting testis functions, preventing and / or treating oligospermia and asthenospermia and protecting male fertility by directly utilizing the plant fruit pulp.

The invention discloses a composition capable of assisting in treatment of infertile symptoms. The composition is prepared by mixing the following main components of a wood frog tissue extract, coenzyme Q10, haematococcus pluvialis astaxanthin microcapsule powder or haematococcus pluvialis wall-breaking spore powder, lecithin, vitamin C and vitamin E. The composition has the efficacy of nourishingthe kidney, boosting essence, improving essence and blood insufficiency, strengthening sexual functions, increasing sperm number and quality, promoting and improving fecundity, resisting oxidation, eliminating free radicals, resisting weariness, improving organism immunity and the like, the oxidative stress reaction of sperms and ova can be effectively reduced, the quality and the vitality of thesperms and the ova can be improved, the sperm abnormality can be avoided, the genitals inflammation can be alleviated or eliminated, the fecundity can be improved, and the composition is suitable forlong-term auxiliary treatment and is free from side effects on human bodies.

The invention provides a kidney-nourishing essence-strengthening decoction, which has the functions of nourishing kidney and strengthening essence, and has excellent effects for treating infertility caused scant and thin semen of male, poor sperm motility or small sperm number, malesexuality weakness and the like. The decoction is prepared from the following raw medicinal materials: polygonum multiflorum, polygonatum kingianum, prepared rehmannia root, fructus lycii and other 27 medicinal materials, has excellent effect on infertility caused by poor sperm motility or small sperm number, has excellent effect and quick response on male infertility, has the effects of strengthening essence and effectively increasing the number of sperms, and does not have side effect on human bodies due to the pure natural traditional Chinese medicinal components.

The invention relates to a sperm quality detection method and device, computer equipment and a storage medium. The method comprises the following steps: acquiring a plurality of to-be-detected sperm images; detecting the to-be-detected sperm image to obtain a sperm morphology detection result of sperms in the to-be-detected sperm image; detecting each to-be-detected sperm image to obtain a sperm number detection result and a sperm position detection result corresponding to each to-be-detected sperm image; determining the sperm concentration according to the sperm number detection result of the plurality of to-be-detected sperm images; and generating a sperm motility detection result according to the sperm position detection results of the plurality of to-be-detected sperm images. By adopting the method, the sperm morphology detection result and the conventional sperm detection result can be quickly obtained, so that the working process of sperm quality detection is greatly simplified, and the sperm quality detection efficiency is improved.

The invention provides a method for evaluating the quality of sperm obtaining energy in vitro. The method specifically includes the following steps of A, collecting a fresh liquefied sperm specimen, washing sperm with a phosphate buffer or a BWW culture solution, sufficiently removing residual seminal plasma components, and separating out sperm in a centrifugal mode; B, adding the BWW culture solution to sperm separated out in the step A, obtaining upstream sperm through an upstream method, counting the number of sperm, incubating sperm for 3-4 hours in a CO2 cell culture box with 5% of in-vitro energy obtaining liquid, conducting centrifugal separation on the obtained supernate, and collecting supernate as a to-be-tested sample; C, measuring the activity of neuraminidase in sperm energy obtaining liquid, and finally obtaining the value of neuraminidase activity / sperm number to evaluate the quality of sperm. By detecting the activity of neuraminidase in sperm in-vitro energy obtaining liquid, diagnosis basis and clinical queries are provided for male infertility patients of unknown clinical reasons.

The invention provides a novel application of plant seed oil. The invention relates to application of plant seed oil in testis function protection, application of plant seed oil in prevention and / or treatment of oligospermia and asthenospermia, application of plant seed oil in protection of male fertility and application of plant seed oil in prevention and / or treatment of testis tissue lesion, testosterone level decline, testis function decline, sperm number reduction, and asthenospermia caused by metabolic disorder. The sperm motility rate is reduced; the sperm morphology is abnormal. The plant seed oil can effectively prevent and treat symptoms such as testicular tissue lesion, testosterone level decline, testicular hypofunction, sperm number reduction, sperm motility rate reduction and sperm morphology abnormality caused by metabolic disorder. The application provides a basis for protecting testis functions, preventing and / or treating oligospermia and asthenospermia and protecting male fertility by directly utilizing the plant seed oil.

The invention discloses a sperm-motility grading counting method, and particularly relates to the technical field of sperm counting. The sperm-motility grading counting method mainly includes the steps that seminal fluid is diluted with fixing diluent and TBS buffer liquid respectively, counting is carried out respectively, and therefore the A-liquid total sperm number and the B-liquid total NP and IM number are obtained; after errors are verified, the number of active (PR) sperms can be obtained through calculation, and the motility of the sperms is determined. By means of the sperm-motilitygrading counting method, sperm movement and the interference in counting when the sperm movement grade is judged can be avoided, the grading difficulty of the motility is reduced to a great degree, and accurate motility grading results are obtained.

The invention discloses a solution for sperm detection. The solution for sperm detection of the invention comprises hyaluronic acid and L-carnitine, and can be used for sperm-cervical mucus penetration tests. Thereinto, the hyaluronic acid is used as a substitute of human cervical mucus, and L-carnitine can protect sperm motility. Based on conditions that patients with oligozoospermia / asthenospermia have less sperm numbers and low sperm motility, the invention is further improved, and a sperm detection solution for patients with oligozoospermia / asthenospermia is prepared. The sperm detection solution for patients with oligozoospermia / asthenospermia has high sensitivity, and a high concentration of L-carnitine, and the L-carnitine can increase the oxidative damage resistance of sperms and protect sperm motility.

The medicine comprises the following components by weight: 2 to 40 g of caulis spatholobi, 1 to 30 g of radix bupleuri, 1 to 30 g of radix paeoniae alba, 1 to 30 g of radix paeoniae rubra, 1 to 30 g of herba lycopi, 2 to 50 g of motherwort herb, 1 to 30 g of radix achyranthis bidentatae, 1 to 30 g of artemisia anomala, 1 to 30 g of sappan wood, 1 to 30 g of glossy privet fruit, 1 to 30 g of raspberry, 1 to 50 g of fructus lycii, 3 to 50 g of pollen typhae, 1 to 30 g of semen cuscutae and 0.5 to 30 g of ginseng. The method can promote maturation of a plurality of follicles, the number of ova is increased in the same sperm density environment, that is, the number of sperms is relatively increased, because one ova is not fertilized and the other ova still has a fertilized opportunity. The traditional Chinese medicine composition can be used for treating female anovulation symptoms, can also be used for assisting in treating male sperm abnormality infertility, and can be used for treating male sperm abnormality infertility, immune infertility and infertility with unknown reasons when being used by men and women at the same time.

The invention relates to the technical field of biological medicines, in particular to application of a TRPV4 agonist in preparation of a medicine for treating and / or preventing male infertility. Experiments show that the TRPV4 agonist (such as GSK1016790A) can improve the sperm number and the sperm motility rate of a spermatogenesis disorder model mouse and reduce the sperm malformation rate of the mouse, so that the TRPV4 agonist is used for preparing the medicine for enhancing the spermatogenesis function of male animals or human beings, provides a new candidate medicine for treating male infertility, and has better economic significance.

Methods for improving the functionality and / or fertility of sperm, for example, by enhancing motility and / or extending the lifespan of sperm by subjecting the isolated sperm to a starvation protocol and / or ionophore are provided. Such methods may be used in, for example, artificial insemination to reduce the number of sperm needed for insemination and to improve conception rates.

Methods for improving the functionality and / or fertility of sperm, for example, by enhancing motility and / or extending the lifespan of sperm by subjecting the isolated sperm to a starvation protocol and / or ionophore are provided. Such methods may be used in, for example, artificial insemination to reduce the number of sperm needed for insemination and to improve conception rates.