22 results about "Sperm Numbers" patented technology

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

The invention discloses an application of gallnut tannic acid as an additive in boar production. The application of the gallnut tannic acid as the additive comprises the application of the gallnut tannic acid as a feed additive in improving the reproductive performance of adult breeding boars and the application of the gallnut tannic acid as a semen diluent additive in improving the normal-temperature preservation quality of boar semen. The feed additive can improve the reproductive performance of adult breeding boars, can significantly improve the sperm density, the total sperm number and the effective sperm number of the boars after being fed for 12 weeks, and improves the oxidation resistance of sperms, sperms and serum; the semen diluent additive can improve the normal-temperature preservation quality of the boar semen, and the sperm motility can still reach 61.20% after the boar semen is preserved for 7 days.

This invention discloses a sperm quality detection sensor and its preparation method, belonging to the field of detection and sensing technology. It includes an electrode with an HA functional layer modified on its surface, the HA functional layer having amino active sites; a redox probe that generates an electrical signal with the electrode; and a conductive layer disposed between the electrode and the redox probe for conduction between them. This application specifically binds the HA functional layer to receptors on the surface of healthy sperm in the test semen and employs microcurrent signal processing technology. The specific binding of the HA functional layer to the receptors affects the current between the electrode and the redox probe. The change in current of the sperm quality detection sensor characterizes the number of healthy sperm in the test semen. This sperm quality detection sensor overcomes the limitations of morphological interpretation in traditional detection technologies, significantly reducing the size of sperm detection equipment and improving the accuracy of sperm quality detection.

The present invention introduces a groundbreaking solution in the field of male contraception-a reversible male birth control implant device. The device offers enhanced flexibility in family planning by controlling the amount of sperm ejaculated. It consists of a tubular component surgically inserted into each severed vas deferens, with a wireless receiver attached. The wireless receiver receives external wireless signals and enables the device to switch between a state that permits the flow of sperm and a state that impedes it. The device also features an outlet for the safe elimination of obstructed sperm. The use of biocompatible materials and compatibility with medical imaging devices, such as MRI, ensures safety and usability. This innovative device empowers individuals with convenient and discreet control over their fertility, revolutionizing male contraception and opening new possibilities in reproductive health.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

The invention discloses animal feed containing Chinese prickly ash seed meal and application of the animal feed, and belongs to the field of feed additives. The invention provides a feed additive which comprises compound bacteria and Chinese prickly ash seed meal, the compound bacteria are composed of bacillus coagulans powder and bacillus reuteri powder, and the mass ratio of the bacillus coagulans powder to the bacillus reuteri powder is (1-4): (1-4). The feed additive is added into chicken daily ration to prepare chicken feed, and experimental verification shows that the chicken feed can remarkably improve the semen volume, sperm motility, sperm density and effective sperm number of breeder cocks and reduce the sperm malformation rate, and is beneficial to improving the reproductive performance of the breeder cocks.

The present invention refers to a biomass of T. chuii and a pharmaceutical composition comprising a biomass of T. chuii for use in the treatment of infertility in a male subject. It also refers to a method for increasing the quality of the semen of a male subject having normozoospermia, not having hypospermia and not having a disorder characterized by a high percentage of sperm DNA fragmentation (SDF), that comprises the administration of a biomass of T. chuii.

The invention relates to the technical field of animal model construction, in particular to a construction method and application of a gene editing mouse model for studying calcium library function and low fertility in mature sperms.The model is characterized in that tamoxifen is used for inducing, a Ddx4ERT2 promoter is used for driving Cre recombinase expression, three subtypes of IP3R are specifically knocked out in sperms at the same time, and the mouse model is obtained. The method is used for researching the action and mechanism of the subtype in male fertility. After the model mouse is subjected to two rounds of induced knockout, the fertility is severely damaged, the sperm quantity and movement level are reduced, and the acrosome reaction is abnormal, so that the model is a good model for evaluating the sperm dysfunction, and is expected to be used for screening treatment drugs for improving the sperm dysfunction, especially male sterility diseases caused by advanced acrosome reaction in the future. In addition, through screening optimization of an IP3R agonist and a calcium signal detection platform, it is further confirmed that the mouse model is a good model for studying sperm calcium library mediated calcium signals.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to sperm-producing decoction as well as a preparation method, application and a product thereof. The sperm-producing decoction is prepared from the following raw materials in parts by weight: 5 to 15 parts of radix salviae miltiorrhizae, 5 to 15 parts of rhizoma chuanxiong, 5 to 15 parts of radix angelicae sinensis, 10 to 25 parts of herba epimedii, 10 to 25 parts of fructus cnidii, 10 to 25 parts of semen cuscutae, 5 to 15 parts of fructus rubi and 5 to 15 parts of fructus schisandrae chinensis. The spermatogenic decoction can improve testis injury caused by an anti-inflammatory and anti-immune drug tripterygium wilfordii hormone, improve oxidative injury of testis tissues, regulate the expression level of antioxidant stress related proteins Nrf2 and HO-1, improve the phenomena of testis diminishment, seminiferous tubule damage, sperm number reduction, sperm motility weakening, testis spermatogenic cell apoptosis and the like, and can be used for preparing the anti-inflammatory and anti-immune drug. And the expression level of apoptosis-related proteins Bcl-2 and Bax of the testicular spermatogenic cells is regulated.

The application belongs to the technical field of biological medicine, and particularly relates to application of a reagent for inhibiting PPAR alpha expression in preparation of a medicine for treating male infertility diseases, wherein a PPAR alpha inhibitor (GW6471) or an active oxygen scavenger (NAC) is given to male mice exposed to cadmium water, it is found that the number of sperm in a Cd+GW6471 group is higher than that in a Cd+NAC group, the serum testosterone level in the Cd+GW6471 group is also higher than that in the Cd+NAC group, and the results of testicular tissue pathological staining also show that GW6471 can more significantly restore the decrease in the number of mature spermatogenic tubules caused by Cd, GW6471 restores the decrease in the number of SYCP3 positive spermatocytes caused by Cd, so that the sperm production efficiency is restored, and therefore the application of the reagent for inhibiting PPAR alpha expression in preparation of the medicine for treating male infertility diseases is proposed.

The invention belongs to the technical field of biological medicines, and particularly relates to application of Fatostatin in preparation of a medicine for preventing and / or treating male reproductive dysfunction. Through an orchitis mouse model, it is proved that Fatostatin can increase the testis weight of an orchitis mouse and significantly improve abnormal sperm quality and abnormal sperm number of the orchitis mouse. According to the application, the Fatostatin is used for preventing or treating the male reproductive dysfunction for the first time, an ideal treatment effect is achieved, a new prevention and treatment choice is provided for clinical application and treatment of male sterility, especially male sterility caused by inflammation infection, and the application has extremely high application value and social benefits.

The invention belongs to the technical field of medicines, and particularly relates to application of a Chinese chive seed extract in preparation of a medicine for treating oligoasthenospermia. Based on network pharmacology prediction and animal experiment verification, it is found that by activating a PI3K / AKT signal channel, testicular cell apoptosis is inhibited, spermatogenesis disorder is improved, and therefore the sperm number and vitality of an oligoasthenospermia model rat are remarkably improved. Clinical treatment research in combination with levocarnitine oral liquid proves that the traditional Chinese medicine composition can reduce the sperm DFI level and improve the semen quality. Animal research proves that the compound can regulate the level of serum testosterone (T), reduce the levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH), repair pathological damage of testis tissues, and antagonize testis cell apoptosis induced by tripterygium glycosides by up-regulating protein expression of p-PI3K / PI3K, p-AKT / AKT and Bcl-2.

The invention discloses application of lycium barbarum red in treating male reproductive toxicity, and belongs to the technical field of biological medicine. The invention provides application of lycium barbarum red in preparation of a medicine for treating male reproductive toxicity. The male reproductive toxicity is caused by exposure to benzophenone substances or exposure to diethylstilbestrol. Animal experiments find that the lycium barbarum red can effectively improve testis function damage induced by benzophenone substance BP-3 or estrogen diethylstilbestrol, improve seminiferous tubule damage degree, increase seminiferous epithelium height, increase sperm number, spermatogonium and primary spermatoblasts, improve spermatogenic ability, increase pregnancy rate, improve sperm yield, improve sperm quality and improve sperm quality. The growth and development of offspring are promoted.

The invention discloses a method for establishing an oligoasthenospermia animal model by utilizing flunarizine and application of the method, and belongs to the technical field of biological medicines. The method comprises the following steps: randomly grouping SPF-level experimental animals; injecting a flunarizine FNZ solution into the abdominal cavity continuously for 7 days; and verifying the oligospermia and asthenozoospermia indexes. In the animal model constructed by the method, the number and activity of sperms in the epididymis of an experimental animal are reduced, the mitochondrial membrane potential of the sperms is reduced due to FNZ, and ATP production is reduced, which indicates that the FNZ reduces the quality of the sperms. Therefore, the method for constructing the oligoasthenospermia model provides a proper animal model for researching male infertility diseases such as oligoasthenospermia and the like; the animal model can be used for animal experiments and new drug research and development for researching and developing occurrence and development mechanisms of male infertility diseases.

The invention discloses a method for constructing an azoospermia mouse model by knocking out a germ cell EIF5A gene and application of the azoospermia mouse model, the construction method is to knock out the EIF5A gene on a mouse germ cell genome, and the knockout is carried out by using a Cre-LoxP system. The azoospermia mouse model constructed by the invention can be used for research on non-obstructive azoospermia and corresponding drug research and development. The model mouse shows that the testis weight and volume are reduced, the spermatogenic epithelium is atrophic, spermatogonium cells cannot complete meiosis completely, germ cells are massively deleted, and the sperm quantity is reduced.

The invention discloses a method for constructing an azoospermia mouse model by knocking out a germ cell Sno1 gene and application of the azoospermia mouse model, the construction method is to knock out the Sno1 gene on a mouse germ cell genome, and the knockout is carried out by using a Cre-LoxP system. The azoospermia mouse model constructed by the invention can be used for research on non-obstructive azoospermia and corresponding drug research and development. The model mouse shows that the testis weight and volume are reduced, spermatogenic epithelium atrophy occurs, spermatogonium cells do not enter meiosis, germ cells are massively deleted, and the number of sperms is reduced.

The invention discloses an additive for improving the semen quality of Bama miniature pigs. The additive is herba epimedii. According to the invention, a single formula of epimedium is used as an additive to be added into the basic ration for feeding the Bama miniature pigs, so that the semen collection amount, sperm motility, sperm density and linear motion sperm number of the breeding boars of the Bama miniature pigs can be remarkably improved, and the sperm malformation rate can be remarkably reduced; meanwhile, biochemical indexes such as total protein, albumin, globulin or total antioxidant capacity, ABTS free radical scavenging rate and the like of seminal plasma in the semen can be improved, so that the semen quality of the Bama miniature pigs is comprehensively improved, the number of healthy piglets born by the Bama miniature pigs is finally improved, and the reproductive capacity and production benefits of the Bama miniature pigs are improved.

The invention discloses a sperm quality detection sensor and a preparation method thereof, and belongs to the technical field of detection sensing, the sperm quality detection sensor comprises an electrode, the surface of the electrode is modified with an HA functional layer, and the HA functional layer has amino active sites; the redox probe and the electrode generate an electric signal; and the conductive layer is arranged between the electrode and the redox probe and is used for conducting between the electrode and the redox probe. According to the application, the HA functional layer is specifically combined with the healthy sperm surface receptor in the to-be-detected sperm, and the current between the electrode and the redox probe is influenced by the specific combination of the HA functional layer and the receptor by adopting a micro-current signal processing technology; the number of healthy sperms in the to-be-detected semen is represented through the current change of the sperm quality detection sensor, the sperm quality detection sensor breaks through the limitation of morphological interpretation in a traditional detection technology, the size of sperm detection equipment can be greatly reduced, and the sperm quality detection precision is improved.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a reagent for inhibiting PPARalpha expression in preparation of a medicine for treating male infertility diseases, a PPARalpha inhibitor (GW6471) or an active oxygen scavenger (NAC) is given to male mice exposed to cadmium water, it is found that the sperm number of a Cd + GW6471 group is higher than that of a Cd + NAC group, the serum testosterone level of the Cd + GW6471 group is also higher than that of the Cd + NAC group, and the PPARalpha inhibitor (GW6471) or the active oxygen scavenger (NAC) is applied to the male mice exposed to cadmium water. The result of pathological staining of the testis tissue also shows that the GW6471 can more obviously recover the reduction of the number of mature seminiferous tubules caused by Cd, and the GW6471 recovers the phenomenon of reduction of the number of SYCP3 positive spermatocytes caused by Cd, so that the spermatogenesis efficiency is recovered, and the spermatogenesis effect is improved. Therefore, the invention provides the application of the reagent for inhibiting the expression of the PPARalpha in preparing the medicine for treating the male infertility disease.

The invention belongs to the technical field of aquaculture, discloses a method for promoting the gonad development of hippocampus kelloggi, and particularly discloses a feed additive which comprises an antioxidant composition and Pseudodartsia andersonii. The feed additive provided by the invention can promote gonad development, shorten testis and ovary maturation time and improve sperm motility, sperm quantity and ovum quantity for male and female sea horses which are not sexually mature; for sexual maturity male hippocampus, sperm recovery can be accelerated, sperm motility is improved, and sperm quantity is increased. By feeding a feed containing the feed additive, the egg laying amount and sperm motility of the hippocampus can be remarkably improved, and the reproduction cycle is shortened, so that the reproduction rate of the hippocampus is effectively improved, population propagation is promoted, and a solid foundation is laid for large-scale breeding of the hippocampus.

Methods for improving the functionality and / or fertility of sperm, for example, by enhancing motility and / or extending the lifespan of sperm by subjecting the isolated sperm to a starvation protocol and / or ionophore are provided. Such methods may be used in, for example, artificial insemination to reduce the number of sperm needed for insemination and to improve conception rates.

The Mogroside extract has the effect of improving the damage of the heat stress boar's reproductive capacity. Research shows that, in the process of heat stress of boar, by adding Mogroside extract in the basic diet, the damage of reproductive capacity caused by heat stress can be effectively improved. The test data shows that the Mogroside extract can significantly improve the sperm density, effective sperm number, sperm motility and reduce the sperm abnormality rate of boar; significantly increase the content of a-glucosidase, lactate dehydrogenase, acrosome enzyme and hyaluronidase in the semen, and increase the concentration of testosterone in the serum, enhance the energy supply, structural integrity, functional maturity and fertilization capacity of sperm. Therefore, the Mogroside extract has the potential for further development in the treatment and prevention of heat stress boar's reproductive capacity damage, and can be used for preparing the medicine for improving the heat stress boar's reproductive capacity, so as to further develop the health care function and medicinal value of Siraitia grosvenorii.