Application of CDR1 gene methylation in asthenozoospermia diagnostic agent and kit

A diagnostic kit and methylation technology, applied in the field of human reproductive health, can solve the problems of accurately diagnosing subjects and less methylated regions

Pending Publication Date: 2020-02-14
深圳市龙华区人民医院
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Problems solved by technology

[0004] However, there are very few reported methylated regions that can be used as molecular markers of asthenospermia, and it is difficult to accurately diagnose whether the subject has asthenospe

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  • Application of CDR1 gene methylation in asthenozoospermia diagnostic agent and kit
  • Application of CDR1 gene methylation in asthenozoospermia diagnostic agent and kit
  • Application of CDR1 gene methylation in asthenozoospermia diagnostic agent and kit

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Embodiment Construction

[0015] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0016] 1. Sperm separation

[0017] Seven asthenospermia volunteers (group A) and eight healthy volunteers (group N) were recruited, and sperm samples were obtained from the volunteers for genome-wide sequencing of methylation levels. Computer-assisted sperm analysis (CASA) was performed using a Sperm Class Analyzer, and sperm morphology was assessed by Diff-Quik rapid staining. Sperm cells with different vigor were separated by the following method: centrifuged at 300×g for 20 min to prepare discontinuous density gradient fractions (divided into 47.5, 57, 76 and 95% concentration layers), and from the 95% concentration layer (fraction a) High motility sperm cells were collected, and low motility sperm cells were c...

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Abstract

The invention relates to an application of a methylation level of a human CDR1 gene promoter region in preparation of a detection agent for detecting sperm motility. The human CDR1 gene promoter region is located in a region corresponding to the 139866223 to 139868923 sites of a human X chromosome. The analysis and research are carried out on the basis of a whole genome methylation sequencing result, the verification shows that the methylation level of the human CDR1 gene promoter region has significant difference between an asthenozoospermia patient and a healthy person, and the human CDR1 gene promoter region can be used as a molecular marker to diagnose whether a subject suffers from asthenozoospermia or not. When the methylation rate of the region is higher than 0.63, the probability that the subject suffers from asthenozoospermia is high. The molecular marker can provide auxiliary evaluation information for diagnosis of asthenozoospermia, and is combined with other diagnosis indexes to identify whether the subject suffers from asthenozoospermia or not.

Description

technical field [0001] The invention relates to the field of human reproductive health, more particularly, relates to the application of the methylation level of the promoter region of human CDR1 gene in the preparation of diagnostic agents for asthenospermia, and a diagnostic kit for asthenospermia. Background technique [0002] More than 40% of infertile men were detected to suffer from asthenospermia. The etiology of asthenospermia is multifactorial, for example, it may be closely related to endocrine disorders, environmental factors, life experiences and even aging. Gene knockout and mutation studies in mice have shown that mutations in some genes are associated with altered sperm motility, but none of these genes have been shown to be associated with sperm motility in human studies. Although nearly 50% of infertility cases are believed to be caused by genetic defects, in some cases of male infertility, genetic factors do not appear to be the main factor. Accumulating ...

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/154
Inventor 杜野唐建新杜新雅黄小明武斌谢春
Owner 深圳市龙华区人民医院
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