109results about How to "Achieve precise quantitation" patented technology

This invention discloses one fluorescent emulsion quantitative diagnostic apparatus bar and its process method, wherein, the test bar comprises orderly on the underlay earth connection sample pad, glass fiber film, cover film and water absorptive water, wherein, the cover film is set with cover antibody or antigen test area and anti-mouse control area; the said glass fiber film is coated with label protein. the process method comprises the following steps: emulsion covalence activating; fluorescence emulsion micro particle label protein processing; antigen or antibody covering onto aqua fortis fiber film and its enclosing; on underlay orderly connecting sample pad, glass fiber film of the protein, cover film and water absorptive paper to get the test board.

The invention relates to a reagent kit for detecting troponin I and a preparation and use method thereof. The reagent kit for detecting the troponin I comprises a base plate, a sample pad, a cross-linked substance pad, a connecting pad, a nitrocellulose membrane and absorbent paper which are connected sequentially are arranged on the base plate from one end to the other end, the cross-linked substance pad envelops cross-linked substances of fluorescent silica nanoparticle-anti-troponin I monoclonal antibodies, the nitrocellulose membrane is provided with a detection band and a quality control band, the detection band is arranged on one side close to the connecting pad and contains anti-troponin I monoclonal antibodies, and the quality control band is arranged on the other side away from the connecting pad and contains goat anti-mouse IgG (immunoglobulin G). The reagent kit for detecting the troponin I can achieve quantitative detection and high-sensitivity detection only by adopting a portable fluorescence detector, is simple in use, high in detection speed and low in cost and can be used for bedside diagnosis.

The invention discloses a data flow concept drift detection method and system. The method analyzes a cluster center, cluster disappearance and cluster new increasing to detect the level of concept drift. The system comprises a cluster center analyzing unit, a cluster disappearance analyzing unit, a cluster new increasing and a concept drift level analyzing unit. The data flow concept drift detection method and system can recognize concept drift from multiple aspects, achieves accurate quantization on concept drift evaluation indexes of a data set to be detected, can comprehensively analyze concept drift situations and can accurately detect the level of concept drift. The data flow concept drift detection method and system is applied to detection of data evolution.

The application provides a detecting device for a large ring and a ring detecting method, and belongs to the technical field of ultrasonic nondestructive testing. The detecting device comprises the following parts of a water tank equipped with a coupling agent and used for placing a ring; a rotating mechanism rotatably installed in the water tank, wherein the ring is arranged on the rotating mechanism; a bridge, of which both ends are arranged on peripheries of two opposite sides of the water tank and capable of moving along the length direction of the two side walls of the water tank; and an ultrasonic scanning mechanism comprising a sliding trolley and an ultrasonic detecting assembly, wherein the sliding trolley is movably mounted on the bridge in the axial direction of the bridge and located above the water tank, and the ultrasonic detecting assembly is mounted up and down on the sliding trolley for detecting internal defects in the ring. The detecting device can accurately and comprehensively test rings, and can adapt to the detection of the rings of different sizes, thereby improving the detection efficiency of the rings and the accuracy of detecting the data.

The invention discloses a floating type direct extrusion casting mold and an implementing method thereof. A central cone of the mold is embedded in an upper mold; a movable ring is embedded in a lower mold; the movable ring is placed on a movable ring cushion block; the movable ring cushion block is placed on a lower mold base; a movable ring limiting block is fixedly connected to the lower mold base; and a cushion block connecting head is fixedly connected with the movable ring cushion block and matched with a hydraulic cylinder connector by a connector rotating shaft. The upper mold moves downwards, so that an alloy melt is full of a cavity and the alloy melt is extruded; meanwhile, the movable ring and the movable ring cushion block are extruded; the movable ring cushion block rotates under a pressure, so that the movable ring moves downwards along with the upper mold; and excessive alloy melt flows out from an overflow groove. According to the invention, the movable ring is rigidly supported by the movable ring cushion block to rotate along with the movable ring cushion block and the movable ring can stably move downwards, so that the alloy melt uniformly flows out from the overflow groove; therefore, the quality of a cast is guaranteed. The floating type direct extrusion casting mold disclosed by the invention is especially suitable for manufacturing medium and large sized non-ferrous alloy casts by using a plurality of casting methods.

The invention discloses a rice micro-ridge mixed sowing cultivation method. The method comprises the following steps: S1, draining water in the mature period of preceding crops until a harvester can work; S2, harvesting the preceding crops, reserving stubbles, smashing straw of the preceding crops and then scattering smashed straw on the reserved stubbles so as to form rhizosphere layers for ricegrowth; S3, performing ditching in the field to form ecological ditches; and S4, flattening the vertical stubbles and the crushed straws on the compartment surface to form a lower cushion surface, forming a seed, fertilizer and mud compound into a ridge shape, enabling the compound to fall on the lower cushion surface to form ecological ridges, and forming a plurality of ecological ridges betweenadjacent ecological ditches, wherein the seed, fertilizer and mud compound is obtained by uniformly stirring and mixing rice seeds, a chemical fertilizer and mud in a mass ratio of (6-14):(50-70):(6000-10000). Firstly, the ecological ridges are used as a medium to wrap organisms and vegetative bodies and is heat-insulating, moisture-preserving, water-permeable and air-permeable; and secondly, theecological ridges are used as a donor to provide water nutrition for organism vegetative mass. The seeds which are subjected to accelerating germination and budding in the ridges grow faster than weedseeds in the ridges and outside the ridges, and roots and leaves grow smoothly.

The invention provides an automatic degassing device and device for separating dissolved gases in a transformer oil sample and belongs to the technical field of transformer oil sample analysis. The automatic degassing device comprises a constant-temperature tank, a sealing container, a mechanical oscillation degassing module, an automatic metering and gas sampling module, a three-dimensional manipulator module and a control module. According to the automatic degassing device for separating the dissolved gases in the transformer oil sample, the sealing container is grasped and taken back troughthe three-dimensional manipulator module, excessive oil samples inside the sealing container can be discharged, sample gases inside the sealing containers can be sucked out, under cooperation of thethree-dimensional manipulator module and the automatic metering and gas sampling module, the oil sample inside the sealing container is precisely quantified, a carrier gas of a preset size is replenished, separated gases are sucked, oscillation degassing of the oil sample is achieved through the mechanical oscillation degassing module, and automatic control from quantitative metering of the oil sample, gas replenishing, constant-temperature oscillation degassing and separated gas transferring to quantitative metering of gases can be achieved through the control module.

The invention provides a method for representing carbonate rock non-compositionality selective reservoir spaces. The method includes steps of 1, carrying out total rock core X-ray three-dimensional scanning tests on samples and forming two-dimensional grayscale images; 2, analyzing and processing two-dimensional grayscale slices of the scanned samples and recognizing fracture and pore developmentfeatures of the samples by the aid of threshold segmentation processes; 3, carrying out multi-scale fusion by the aid of processes for direct construction on the basis of grayscale images and processes for construction on the basis of pore network models, then implementing visualization and building three-dimensional space models; 4, representing the carbonate rock non-compositionality selective reservoir spaces and analyzing pore structures; 5, quantitatively representing reservoir spaces of the samples and outputting charts, maps and data. The method for representing the carbonate rock non-compositionality selective reservoir spaces has the advantages that multi-scale and continuous research on rock cores can become reality by the aid of the method, and detailed stratum cognition materials can be provided to geological workers.

The invention discloses a control system for a standardized kitchen of Chinese food, which comprises a plurality of kitchen heating devices, a plurality of Chinese food making containers, an information acquisition machine, and a server having installed computer program, wherein the information acquisition machine and the kitchen heating devices are connected with the server; the information acquisition machine acquires the identification information of Chinese food dish and transmits the identification information to the computer program of the server, and the corresponding cooking technology is distributed to the free kitchen heating device according to the identification information by the computer program so that the kitchen heating device works in corresponding work mode according tothe cooking technology. The system realizes the precision quantization of heat control, proportion of side dishes and seasoning dosage by setting the cooking technology of each Chinese cook dish, thereby guaranteeing uniform taste of each Chinese cook dish and realizing the standardization of cooking; in the progress, the labor requirement of kitchen is reduced, the space of kitchen is saved, andthe cost of labor is reduced.

The invention discloses automatic quantitative power filling equipment for a novel heat tube. The automatic quantitative power filling equipment comprises a rack, a control device, a precise power quantifying mechanism, a lifting power filling mechanism and a rotating worktable mechanism; the precise power quantifying mechanism comprises a power storage box, a power storage box baseplate, hollow cylindrical tubes, power quantifying funnels, an upper fixing plate, middle movable plates, a plurality of powder storage measuring cylinders, a lower fixing plate and the like; the lifting power filling mechanism comprises a compression bar assembly fixing disk, mandrel compression bar assemblies, a copper powder tube fixing disk, replaceable powder cup assemblies, a lifting platform baseplate, alocating cylinder, a lifting cylinder and the like; the rotating worktable mechanism comprises powder filling worktables, lifting worktables, vibration baseplates, damping spring components, a power storing plate and the like; and the control device is connected to the precise power quantifying mechanism, the lifting power filling mechanism and the rotating worktable mechanism. The automatic quantitative power filling equipment has the advantages of high quality, precision, power filling efficiency, automation degree and universality, easy operation, low labor intensity and small dust.

An ammonia nitrogen online monitor based on the distillation-titration method is composed of a sampling and pretreatment unit, a quantitative unit, a distillation unit, an absorption titration unit, a post-processing unit, and a PLC controller. The present invention uses liquid buffer solution instead of solid MgO to realize the automatic addition of pH regulator; the present invention adopts an automatic distillation device with adjustable electric heating power and controllable distillation rate, which improves the distillation efficiency and increases the safety and reliability of the instrument The present invention adopts a liquid automatic metering device with freely adjustable liquid volume to realize accurate quantification of water samples and absorption liquids; the present invention uses a pH potential detection electrode to automatically determine the titration end point, which increases the sensitivity and accuracy of the instrument, And the measurement range of the instrument is expanded, so that the present invention can simultaneously adapt to the on-line automatic detection of industrial sewage, domestic sewage, rivers, lakes and other surface waters.

The invention discloses an in-situ ultrasonic detection tool for an impeller blade root groove of a gas turbine compressor. The in-situ ultrasonic detection tool comprises a left clamping arm, a rightclamping arm, a sliding guide rail, a guide device and an L-shaped bracket; the left clamping arm and the right clamping arm are oppositely arranged and fixedly connected with the sliding guide railthrough guide rail grooves in a meshed mode, telescopic devices are arranged in the middle of the left clamping arm and the middle of the right clamping arm respectively, and a left clamping arm fixing claw and a right clamping arm fixing claw are arranged at the end of the left clamping arm and the end of the right clamping arm respectively. The guide device is meshed with the sliding guide railthrough the guide rail grooves; the L-shaped bracket comprises an L-shaped bracket vertical arm and an L-shaped bracket vertical arm; the lower end of the guide device is connected with the upper endof the L-shaped bracket vertical arm; the L-shaped bracket vertical arm is sleeved with a spring, and a L-shaped bracket horizontal arm is sleeved with a probe clamping device through a support groove. The detection tool is stably fixed, the ultrasonic probe is stably coupled, meanwhile, the ultrasonic probe is accurately scanned and accurately positioned, and in-situ efficient and reliable detection of cracks of the impeller blade root groove of the gas compressor is achieved.

The invention discloses a method for determining the content of methcathinone in a sample by high performance liquid chromatography. The method includes the steps of: (1) preparation of a working solution; (2) preparation of a sample solution; (3) setting of detection conditions; and (4) determination of the methcathinone content of the sample. The method provided by the invention adopts reversed-phase chromatography isocratic elution, can complete analysis within 12 minutes, has efficient and accurate detection result, can be used for determination of the methcathinone content of the sample, and provides technical support for conviction and sentencing of judicial departments.

The invention discloses a TOFD near surface dead zone defect locating detection method based on wave-type transformation and belongs to the field of nondestructive detection. The method includes that an ultrasonic detection system comprising a TOFD ultrasonic detector, a detection probe, a calibration block and scanning device is adopted for TOFD detection; longitudinal waves emitted by the TOFD probe diffract when encountering defects, and when an incident angle reaches a supercritical value, wave-type transformation of longitudinal wave to transverse wave or transverse wave to longitudinal wave occurs; a defect end point solution model is built according to geometrical relationship among sonic path distances of waves different in type in a scanning image B; a projection distance d' of shortest sonic path distance of transformed waves in the vertical direction and a projection distance S in the horizontal direction between an intersection point of the transformed waves and a shortest sonic path distance position are measured, and then a distance d between a defect end point and a detection face can be calculated by combining the projection distance d' and the projection distance S with a probe central distance 2S, longitudinal wave sonic speed C1 and transverse wave sonic speed Cs through a formula to realize locating of near surface dead zone defects. By the method, original A scanning signals do not need to be extracted for analysis and post-processing; the method is high in operability and high in engineering application value.

The invention belongs to the technical field of biomedical treatment, and specifically relates to a method and a test kit for detecting novel coronavirus. The test kit comprises a primer pair and a probe, wherein the sequences of the primer pair and the probe are as shown in SEQ ID NO. 1-6. The digital PCR detection test kit provided by the invention can reach the minimum detection sensitivity of50 copies/ml, realizes accurate quantification of trace pathogen nucleic acid, can detect pathogen nucleic acid from a large number of background signals, eliminates the dependence on a standard curve, does not need formula conversion and realizes absolute quantification of pathogen copies.

The invention discloses a Tibetan chicken feeding trough and belongs to the field of feeding troughs. The Tibetan chicken feeding trough comprises a food providing area and a feeding area. The feeding area comprises a plurality of feeding trough bodies, a spacing bar is arranged between every two feeding trough bodies, a conveying device is arranged below the feeding trough bodies and penetrates through the feeding area, and the conveying device communicates with the food providing area and the feeding area. The Tibetan chicken feeding trough has the advantages that precise quantification is achieved, it is guaranteed that the maximum edible amounts of Tibetan chickens are the same, the influences of human factors in the feeding process are reduced, and precise food providing is achieved.

The invention discloses an absolute quantitative transcriptome library construction method based on a specific recognition sequence. Fragmented mRNA is used as a template, a first cDNA chain is synthesized under the action of reverse transcriptase by a primer pool with a general joint sequence, a library construction joint with a special recognition UID sequence is added to cDNA 3' end through anenzymatic reaction, so that each cDNA carries a unique sequence label; and finally, PCR amplification is performed by the general library construction joint to obtain an RNA library. The method constructs the RNA library on the basis of single-stranded cDNA by a splint connection method for the first time, at the same time, a UID sequence is used to precisely reduce the cDNA composition before PCRamplification and can realize precise quantification of transcripts. Library construction is performed by the single-stranded cDNA as a raw material, the step of second strand synthesis is omitted, the template loss rate is reduced, the cost and time are saved, and the defect that the prior art can relatively quantify transcripts is thoroughly solved.

The invention relates to a large-scale pelletizing machine, which comprises a frame, a feeding device arranged on the frame, and a coating bucket. The feeding device includes a drug powder bin and a temporary seed storage bin arranged side by side. A spinner is provided, and the center of the bottom of the spinner is connected with a drive shaft, and the drive shaft is installed on the bottom of the coating barrel through the first bearing seat. Since the lower part of the seed temporary storage bin is equipped with a weighing bucket and a load cell, it is convenient The seeds and medicinal powder are mixed and pelletized into the cylinder in proportion to realize accurate quantification and good pelletization quality; the invention transports the coating agent in multiple directions along the circumference of the atomization sleeve, improving the quality of the pelletized seeds; It is also possible to input hot air into the hot air cavity through the hot air port to dry the coated seeds above the spinner to prevent the coated seeds from sticking together.

The invention provides a balance joint with a molecular barcode and a method for quickly constructing a transcriptome library. The balance joint with the molecular barcode is formed by mixing two kinds of balance joints with different lengths, and the balance joint sequentially comprises a sequencing library PCR primer recognition sequence, a molecular bar code sequence and a terminal base sequence from the 5' terminal to the 3' terminal; and the long balance joint also includes an intermediate base sequence. The joint can thoroughly solve the problem of data pollution caused by duplication, non-specific annealing and other reasons generated in the PCR process, and overcomes the problems of transcriptome library construction by using SMART technology product lines and base imbalance duringLane sequencing. The invention also provides a method for quickly constructing the transcriptome library by using the joint and adding an internal reference standard substance, so that the steps aresimplified, and the library construction time is shortened to a great extent; and the internal reference standard substance is added, so that the deviation caused by a data result can be subjected tostandardized correction, and the reliability of subsequent analysis is ensured.