109results about How to "Achieve precise quantitation" patented technology

This invention discloses one fluorescent emulsion quantitative diagnostic apparatus bar and its process method, wherein, the test bar comprises orderly on the underlay earth connection sample pad, glass fiber film, cover film and water absorptive water, wherein, the cover film is set with cover antibody or antigen test area and anti-mouse control area; the said glass fiber film is coated with label protein. the process method comprises the following steps: emulsion covalence activating; fluorescence emulsion micro particle label protein processing; antigen or antibody covering onto aqua fortis fiber film and its enclosing; on underlay orderly connecting sample pad, glass fiber film of the protein, cover film and water absorptive paper to get the test board.

The invention belongs to the technical field of artificial simulation sample preparation testing of geotechnical engineering seabed gas contained deposition, and particularly discloses a high-pressure gas dissolved saturation test device and an application thereof in gas contained soil sample artificial preparation. The device provided by the invention comprises a gas source tank, a reaction kettle, a lifting device, a geotechnique triaxial testing system, a connecting pipeline, a valve and a pressure meter. The artificial simulation preparation of a laboratory gas contained soil sample can be realized without pressure condition restriction by the device; the device is especially suitable for the sample preparation of a high-saturability gas contained deposition soil body in indoor triaxial testing; the gas contained quantity can be controlled artificially and can be quantified accurately; the prepared soil sample is uniform and has preparation repeatability.

The invention relates to the field of insulating oil gas chromatography analysis equipment and in particular to a portable gas chromatograph analysis device comprising a casing; a sample pretreatmentmodule for separating a gas in an insulating oil sample container by an automatic headspace degassing device and quantitatively transferring the same to a chromatographic column box module through a sampling device and to a detector module; the chromatographic column box module including a chromatographic constant-temperature column box and a TCD detector, and used for subjecting the received gassample to chromatographic separation through an internal chromatographic column and transferring the same to the detector module; the detector module including an FID detector and a Ni catalytic converter which convert the component concentration after the chromatographic separation into an electrical signal and feed the electrical signal back to the controller in order that the controller amplifies the electrical signal, performs an analog-to-digital conversion process, and transmits a digital signal to a chromatographic workstation for data processing to obtain final chromatographic data. The portable gas chromatograph analysis device is high in sensitivity, small in volume, convenient to carry, efficient in time, and good in practicality and operability.

The invention discloses a sample quantifying unit and a microfluidic chip comprising the same. The sample quantifying unit comprises a sample injecting tank, a quantifying tank and a sample treatmenttank, wherein the sample injecting tank is communicated with a sample injecting port of the microfluidic chip; the quantifying tank comprises a quantifying zone and a waste liquid zone which are communicated; the quantifying zone comprises a buffer zone, a quantifying subzone and a precipitating subzone which are sequentially communicated; the buffer zone is communicated with the sample injectingtank through a first capillary connecting pipe; the top of the quantifying subzone is communicated with the waste liquid zone; a waste liquid tank is connected to the bottom end of the waste liquid zone; a sample enters the quantifying subzone and the buffer zone from the sample injecting tank by way of buffer zone, and the excessive sample enters the waste liquid zone from the top of the quantifying subzone; the sample treatment tank is connected with the quantifying subzone through a siphon; the sample injecting tank, the quantifying tank and the sample treatment tank are communicated with the atmosphere. The sample quantifying unit can achieve accurate quantification of the sample and reduces the error, so that the detection efficiency and the detection accuracy of the microfluidic chipare significantly improved.

The invention discloses a freeze-drying concentrated glucose detection reagent microsphere and a preparation method thereof. The freeze-drying microsphere comprises a triethanolamine buffer solution, ATP, NADP, hexokinase, 6-glucose dehydrogenase phosphate, a preservative, trehalose, polyethylene glycol 8000, polyethylene glycol 20000, TrionX-100 and the like. The preparation method comprises the following steps: (1) preparing a solution, and performing filtration and degassing; (2) preparing droplets by using a precise quantification liquid separation system, dropwise adding the droplets into liquid nitrogen, and preparing a freezing microsphere; and (3) transferring the freezing microsphere into a freeze dryer, and performing freeze-drying on the freezing microsphere to obtain a freeze-drying detection reagent microsphere. A glucose detection reagent provided by the invention is a spherical granular freeze-drying reagent and can be pre-packaged into a detection chip. Compared with an existing liquid detection reagent, the glucose detection reagent has the advantages that the storage, transportation and the like at the stable room temperature can be realized. Compared with a freeze-drying powder reagent, the glucose detection reagent has the advantages of accuracy in quantification, convenience in packaging treatment and the like.

The invention relates to a reagent kit for detecting troponin I and a preparation and use method thereof. The reagent kit for detecting the troponin I comprises a base plate, a sample pad, a cross-linked substance pad, a connecting pad, a nitrocellulose membrane and absorbent paper which are connected sequentially are arranged on the base plate from one end to the other end, the cross-linked substance pad envelops cross-linked substances of fluorescent silica nanoparticle-anti-troponin I monoclonal antibodies, the nitrocellulose membrane is provided with a detection band and a quality control band, the detection band is arranged on one side close to the connecting pad and contains anti-troponin I monoclonal antibodies, and the quality control band is arranged on the other side away from the connecting pad and contains goat anti-mouse IgG (immunoglobulin G). The reagent kit for detecting the troponin I can achieve quantitative detection and high-sensitivity detection only by adopting a portable fluorescence detector, is simple in use, high in detection speed and low in cost and can be used for bedside diagnosis.

The invention provides a method for preparing a proliferation internal label based on a DNA random reorganization technique, and belongs to the field of the biological technology. The method comprises the following steps that: according to a target gene design, a specific detection primer and a TaqMan probe are obtained; according to DNA sequences on the probe combination part of a target fragment, DNA random reorganization software randomly generates reorganization sequences, and the generated reorganization sequences replace the DNA sequences on the probe combination part of the target fragment respectively to generate corresponding proliferation internal label sequences to be screened; by comparing the fluorescent probe design software with the BLAST-N software, the proliferation internal label sequences are screened to obtain the sequence which has the highest software evaluation value and is not homologous to the sequences of other pathogenic microorganism genomes, and the sequence is used as the proliferation internal label sequence; the proliferation internal label sequence obtained from the third step is cloned to a vector to construct the vector containing the proliferation internal label sequence; and the obtained proliferation internal label sequence is detected. The method can help to display the existence of the inhibition phenomenon in detection, thereby improving the accuracy rate of the detection result.

The invention discloses a data flow concept drift detection method and system. The method analyzes a cluster center, cluster disappearance and cluster new increasing to detect the level of concept drift. The system comprises a cluster center analyzing unit, a cluster disappearance analyzing unit, a cluster new increasing and a concept drift level analyzing unit. The data flow concept drift detection method and system can recognize concept drift from multiple aspects, achieves accurate quantization on concept drift evaluation indexes of a data set to be detected, can comprehensively analyze concept drift situations and can accurately detect the level of concept drift. The data flow concept drift detection method and system is applied to detection of data evolution.

The invention discloses a method and a device for realizing direct extrusion casting by utilizing closed mould pouring. The method comprises the following steps of: premoulding a convex mould and a concave mould to form a closed filling space; filling inertia protective gas into the filling space; introducing an alloy melt into a charging barrel by utilizing a transporting system; pressing the alloy melt into a mould cavity by a pressure head, thus filling is completed; and carrying out direct extrusion by adopting the way that the convex mould moves downward, and causing the alloy melt to be crystallized and solidified under pressure. The device for realizing the method has the main connection relationship that: the convex mould is fixedly connected with a movable cross beam of a casting machine; a convex mould limit support is fixedly connected with a convex mould base; a convex mould limit cushion block and a mould frame are in sliding fit and are connected with a hydraulic cylinder at a cushion block side; and the pressure head is arranged in the charging barrel in a movable fit manner and is connected with a filling / ejecting hydraulic cylinder of the casting machine. The method and the device disclosed by the invention fully utilize a direct extrusion mode and have the advantages that extrusion area is large, extrusion acting force transmission distance is short and uniform and pressure loss is less, and the alloy melt is transmitted by virtue of a closed pipeline, thus oxidation inspiration is reduced.

The application provides a detecting device for a large ring and a ring detecting method, and belongs to the technical field of ultrasonic nondestructive testing. The detecting device comprises the following parts of a water tank equipped with a coupling agent and used for placing a ring; a rotating mechanism rotatably installed in the water tank, wherein the ring is arranged on the rotating mechanism; a bridge, of which both ends are arranged on peripheries of two opposite sides of the water tank and capable of moving along the length direction of the two side walls of the water tank; and an ultrasonic scanning mechanism comprising a sliding trolley and an ultrasonic detecting assembly, wherein the sliding trolley is movably mounted on the bridge in the axial direction of the bridge and located above the water tank, and the ultrasonic detecting assembly is mounted up and down on the sliding trolley for detecting internal defects in the ring. The detecting device can accurately and comprehensively test rings, and can adapt to the detection of the rings of different sizes, thereby improving the detection efficiency of the rings and the accuracy of detecting the data.

The invention discloses a floating type direct extrusion casting mold and an implementing method thereof. A central cone of the mold is embedded in an upper mold; a movable ring is embedded in a lower mold; the movable ring is placed on a movable ring cushion block; the movable ring cushion block is placed on a lower mold base; a movable ring limiting block is fixedly connected to the lower mold base; and a cushion block connecting head is fixedly connected with the movable ring cushion block and matched with a hydraulic cylinder connector by a connector rotating shaft. The upper mold moves downwards, so that an alloy melt is full of a cavity and the alloy melt is extruded; meanwhile, the movable ring and the movable ring cushion block are extruded; the movable ring cushion block rotates under a pressure, so that the movable ring moves downwards along with the upper mold; and excessive alloy melt flows out from an overflow groove. According to the invention, the movable ring is rigidly supported by the movable ring cushion block to rotate along with the movable ring cushion block and the movable ring can stably move downwards, so that the alloy melt uniformly flows out from the overflow groove; therefore, the quality of a cast is guaranteed. The floating type direct extrusion casting mold disclosed by the invention is especially suitable for manufacturing medium and large sized non-ferrous alloy casts by using a plurality of casting methods.

The invention discloses a rice micro-ridge mixed sowing cultivation method. The method comprises the following steps: S1, draining water in the mature period of preceding crops until a harvester can work; S2, harvesting the preceding crops, reserving stubbles, smashing straw of the preceding crops and then scattering smashed straw on the reserved stubbles so as to form rhizosphere layers for ricegrowth; S3, performing ditching in the field to form ecological ditches; and S4, flattening the vertical stubbles and the crushed straws on the compartment surface to form a lower cushion surface, forming a seed, fertilizer and mud compound into a ridge shape, enabling the compound to fall on the lower cushion surface to form ecological ridges, and forming a plurality of ecological ridges betweenadjacent ecological ditches, wherein the seed, fertilizer and mud compound is obtained by uniformly stirring and mixing rice seeds, a chemical fertilizer and mud in a mass ratio of (6-14):(50-70):(6000-10000). Firstly, the ecological ridges are used as a medium to wrap organisms and vegetative bodies and is heat-insulating, moisture-preserving, water-permeable and air-permeable; and secondly, theecological ridges are used as a donor to provide water nutrition for organism vegetative mass. The seeds which are subjected to accelerating germination and budding in the ridges grow faster than weedseeds in the ridges and outside the ridges, and roots and leaves grow smoothly.

The invention provides an automatic degassing device and device for separating dissolved gases in a transformer oil sample and belongs to the technical field of transformer oil sample analysis. The automatic degassing device comprises a constant-temperature tank, a sealing container, a mechanical oscillation degassing module, an automatic metering and gas sampling module, a three-dimensional manipulator module and a control module. According to the automatic degassing device for separating the dissolved gases in the transformer oil sample, the sealing container is grasped and taken back troughthe three-dimensional manipulator module, excessive oil samples inside the sealing container can be discharged, sample gases inside the sealing containers can be sucked out, under cooperation of thethree-dimensional manipulator module and the automatic metering and gas sampling module, the oil sample inside the sealing container is precisely quantified, a carrier gas of a preset size is replenished, separated gases are sucked, oscillation degassing of the oil sample is achieved through the mechanical oscillation degassing module, and automatic control from quantitative metering of the oil sample, gas replenishing, constant-temperature oscillation degassing and separated gas transferring to quantitative metering of gases can be achieved through the control module.

The invention provides a method for representing carbonate rock non-compositionality selective reservoir spaces. The method includes steps of 1, carrying out total rock core X-ray three-dimensional scanning tests on samples and forming two-dimensional grayscale images; 2, analyzing and processing two-dimensional grayscale slices of the scanned samples and recognizing fracture and pore developmentfeatures of the samples by the aid of threshold segmentation processes; 3, carrying out multi-scale fusion by the aid of processes for direct construction on the basis of grayscale images and processes for construction on the basis of pore network models, then implementing visualization and building three-dimensional space models; 4, representing the carbonate rock non-compositionality selective reservoir spaces and analyzing pore structures; 5, quantitatively representing reservoir spaces of the samples and outputting charts, maps and data. The method for representing the carbonate rock non-compositionality selective reservoir spaces has the advantages that multi-scale and continuous research on rock cores can become reality by the aid of the method, and detailed stratum cognition materials can be provided to geological workers.

The invention discloses a control system for a standardized kitchen of Chinese food, which comprises a plurality of kitchen heating devices, a plurality of Chinese food making containers, an information acquisition machine, and a server having installed computer program, wherein the information acquisition machine and the kitchen heating devices are connected with the server; the information acquisition machine acquires the identification information of Chinese food dish and transmits the identification information to the computer program of the server, and the corresponding cooking technology is distributed to the free kitchen heating device according to the identification information by the computer program so that the kitchen heating device works in corresponding work mode according tothe cooking technology. The system realizes the precision quantization of heat control, proportion of side dishes and seasoning dosage by setting the cooking technology of each Chinese cook dish, thereby guaranteeing uniform taste of each Chinese cook dish and realizing the standardization of cooking; in the progress, the labor requirement of kitchen is reduced, the space of kitchen is saved, andthe cost of labor is reduced.

The invention discloses automatic quantitative power filling equipment for a novel heat tube. The automatic quantitative power filling equipment comprises a rack, a control device, a precise power quantifying mechanism, a lifting power filling mechanism and a rotating worktable mechanism; the precise power quantifying mechanism comprises a power storage box, a power storage box baseplate, hollow cylindrical tubes, power quantifying funnels, an upper fixing plate, middle movable plates, a plurality of powder storage measuring cylinders, a lower fixing plate and the like; the lifting power filling mechanism comprises a compression bar assembly fixing disk, mandrel compression bar assemblies, a copper powder tube fixing disk, replaceable powder cup assemblies, a lifting platform baseplate, alocating cylinder, a lifting cylinder and the like; the rotating worktable mechanism comprises powder filling worktables, lifting worktables, vibration baseplates, damping spring components, a power storing plate and the like; and the control device is connected to the precise power quantifying mechanism, the lifting power filling mechanism and the rotating worktable mechanism. The automatic quantitative power filling equipment has the advantages of high quality, precision, power filling efficiency, automation degree and universality, easy operation, low labor intensity and small dust.

The invention relates to an ammonium-nitrogen online monitor based on a distilling-titration method. The ammonium-nitrogen online monitor consists of a sampling and pretreatment unit, a quantifying unit, a distilling unit, an absorption and titration unit, an aftertreatment unit and a PLC controller. A liquid buffer solution is adopted to substitute solid MgO to realize the automatic addition of a pH regulator; an automatic distilling apparatus with adjustable electric heating power and controllable distilling rate is adopted, so that the distilling efficiency is increased, and the safety and reliability of the instrument are improved; an automatic liquid metering apparatus capable of freely adjusting the liquid amount is adopted, so that the precise quantification of a water sample and absorption liquid can be realized; and a pH potential detection electrode is adopted to automatically determine a titration end point, so that the sensitivity and accuracy of the instrument are improved, the measurement range of the instrument is enlarged, and the ammonium-nitrogen online monitor can be simultaneously suitable for the online automatic detection of surface water such as industrial sewage, domestic sewage, rivers and lakes.

The invention discloses a water quality total nitrogen on-line automatic monitoring device. The automatic monitoring device comprises a sampling system (1), a reaction system (2) and a detection system (3) which are orderly connected through pipelines. The automatic monitoring device is characterized by also comprising a PLC control system (4), wherein the PLC control system (4) controls the sampling system (1) to sample water, the sampled water reacts sufficiently in the reaction system (2), and the detection system (3) detects reactant liquid to realize automatic monitoring. Compared with the like products, the device has the advantages of lower failure rate, less maintenance, lower reagent consumption and higher performance price ratio and accuracy.

The invention discloses an in-situ ultrasonic detection tool for an impeller blade root groove of a gas turbine compressor. The in-situ ultrasonic detection tool comprises a left clamping arm, a rightclamping arm, a sliding guide rail, a guide device and an L-shaped bracket; the left clamping arm and the right clamping arm are oppositely arranged and fixedly connected with the sliding guide railthrough guide rail grooves in a meshed mode, telescopic devices are arranged in the middle of the left clamping arm and the middle of the right clamping arm respectively, and a left clamping arm fixing claw and a right clamping arm fixing claw are arranged at the end of the left clamping arm and the end of the right clamping arm respectively. The guide device is meshed with the sliding guide railthrough the guide rail grooves; the L-shaped bracket comprises an L-shaped bracket vertical arm and an L-shaped bracket vertical arm; the lower end of the guide device is connected with the upper endof the L-shaped bracket vertical arm; the L-shaped bracket vertical arm is sleeved with a spring, and a L-shaped bracket horizontal arm is sleeved with a probe clamping device through a support groove. The detection tool is stably fixed, the ultrasonic probe is stably coupled, meanwhile, the ultrasonic probe is accurately scanned and accurately positioned, and in-situ efficient and reliable detection of cracks of the impeller blade root groove of the gas compressor is achieved.

The invention discloses a method for determining the content of methcathinone in a sample by high performance liquid chromatography. The method includes the steps of: (1) preparation of a working solution; (2) preparation of a sample solution; (3) setting of detection conditions; and (4) determination of the methcathinone content of the sample. The method provided by the invention adopts reversed-phase chromatography isocratic elution, can complete analysis within 12 minutes, has efficient and accurate detection result, can be used for determination of the methcathinone content of the sample, and provides technical support for conviction and sentencing of judicial departments.

The invention discloses a TOFD near surface dead zone defect locating detection method based on wave-type transformation and belongs to the field of nondestructive detection. The method includes that an ultrasonic detection system comprising a TOFD ultrasonic detector, a detection probe, a calibration block and scanning device is adopted for TOFD detection; longitudinal waves emitted by the TOFD probe diffract when encountering defects, and when an incident angle reaches a supercritical value, wave-type transformation of longitudinal wave to transverse wave or transverse wave to longitudinal wave occurs; a defect end point solution model is built according to geometrical relationship among sonic path distances of waves different in type in a scanning image B; a projection distance d' of shortest sonic path distance of transformed waves in the vertical direction and a projection distance S in the horizontal direction between an intersection point of the transformed waves and a shortest sonic path distance position are measured, and then a distance d between a defect end point and a detection face can be calculated by combining the projection distance d' and the projection distance S with a probe central distance 2S, longitudinal wave sonic speed C1 and transverse wave sonic speed Cs through a formula to realize locating of near surface dead zone defects. By the method, original A scanning signals do not need to be extracted for analysis and post-processing; the method is high in operability and high in engineering application value.

The invention discloses a magnetic micro particle immunofluorescence kit for quantitatively assaying classical swine fever virus antibody, which consists of classical swine fever E2 antigen immunomagnetic beads, fluorescent substance-labeled goat anti-pig antibody, detergent, enhancement solution, negative control serum and positive control serum. Compared with the RT-PCR (reverse transcription-polymerase chain reaction) method and the ELISA (enzyme-linked immunosorbent assay) method, the magnetic micro particle immunofluorescence kit for quantitatively assaying classical swine fever virus antibody which is disclosed by the invention is easy to operate and suitable for assaying different numbers of samples, and has high assay speed (a result can be obtained in about 20 minutes); and compared with the colloidal gold immunochromatographic strip, the kit has the advantages of higher sensitivity, wider linear range, capability of realizing accurate quantification and the like. The assay result of the kit can accurately reflect the change law of anti-CSFV (anti-classical swine fever virus) serum IgG antibody of the body in the process of vaccination and provide scientific and reasonabletechnical support for pig farmers in preventing and controlling CSF (classical swine fever).

The invention belongs to the technical field of biomedical treatment, and specifically relates to a method and a test kit for detecting novel coronavirus. The test kit comprises a primer pair and a probe, wherein the sequences of the primer pair and the probe are as shown in SEQ ID NO. 1-6. The digital PCR detection test kit provided by the invention can reach the minimum detection sensitivity of50 copies / ml, realizes accurate quantification of trace pathogen nucleic acid, can detect pathogen nucleic acid from a large number of background signals, eliminates the dependence on a standard curve, does not need formula conversion and realizes absolute quantification of pathogen copies.

The invention discloses an absolute quantitative transcriptome library construction method based on a specific recognition sequence. Fragmented mRNA is used as a template, a first cDNA chain is synthesized under the action of reverse transcriptase by a primer pool with a general joint sequence, a library construction joint with a special recognition UID sequence is added to cDNA 3' end through anenzymatic reaction, so that each cDNA carries a unique sequence label; and finally, PCR amplification is performed by the general library construction joint to obtain an RNA library. The method constructs the RNA library on the basis of single-stranded cDNA by a splint connection method for the first time, at the same time, a UID sequence is used to precisely reduce the cDNA composition before PCRamplification and can realize precise quantification of transcripts. Library construction is performed by the single-stranded cDNA as a raw material, the step of second strand synthesis is omitted, the template loss rate is reduced, the cost and time are saved, and the defect that the prior art can relatively quantify transcripts is thoroughly solved.

The invention relates to a large-scale pelletizing machine, which comprises a frame, a feeding device arranged on the frame, and a coating bucket. The feeding device includes a drug powder bin and a temporary seed storage bin arranged side by side. A spinner is provided, and the center of the bottom of the spinner is connected with a drive shaft, and the drive shaft is installed on the bottom of the coating barrel through the first bearing seat. Since the lower part of the seed temporary storage bin is equipped with a weighing bucket and a load cell, it is convenient The seeds and medicinal powder are mixed and pelletized into the cylinder in proportion to realize accurate quantification and good pelletization quality; the invention transports the coating agent in multiple directions along the circumference of the atomization sleeve, improving the quality of the pelletized seeds; It is also possible to input hot air into the hot air cavity through the hot air port to dry the coated seeds above the spinner to prevent the coated seeds from sticking together.

The invention belongs to the field of analytical chemistry, and particularly relates to an ultra-high performance liquid chromatography rapid detection technology, in particular to a method for efficiently and rapidly detecting the content of EGCG in tea polyphenol as a raw material by utilizing the ultra-high performance liquid chromatography detection technology. A C18 chromatographic column isadopted; water-glacial acetic acid-methanol with a certain proportion is used as a mobile phase; the content of the EGCG in the tea polyphenol is rapidly detected in a gradient elution mode; and the theoretical plate number of the EGCG under the chromatographic condition is greater than 16,000, and the separation degree is greater than 3. By adopting the detection method, the EGCG, EC and caffeinerelatively difficult to separate are effectively separated; and the method is short in detection time, high in sensitivity, good in reproducibility, efficient and rapid, and can be applied to the EGCG content detection and quality control.

The invention discloses a double droplet digital PCR absolute quantitative detection kit for European and American porcine reproductive and respiratory syndrome virus. The primer probe combination comprises LV-F1R1P1 and VR2332-F1R1P1, wherein LV-F1R1P1 consists of LV-F1, LV-R1 and LV-P1, the LV-F1 is as shown in a sequence 1, LV-R1 is as shown in a sequence 2, and LV-P1 is as shown in a sequence 3; VR2332-F1R1P1 consists of VR2332-F1, VR2332-R1 and VR2332-P1, VR2332-F1 is as shown in a sequence 4, VR2332-R1 is as shown in a sequence 5, and VR2332-P1 is as shown in a sequence 6. The kit has an important application value on prevention and control of European and / or American porcine reproductive and respiratory syndrome viruses, and is favorable to epidemic situation control from the source and effective prevention of porcine epidemic large-scale outbreak.

The invention provides a balance joint with a molecular barcode and a method for quickly constructing a transcriptome library. The balance joint with the molecular barcode is formed by mixing two kinds of balance joints with different lengths, and the balance joint sequentially comprises a sequencing library PCR primer recognition sequence, a molecular bar code sequence and a terminal base sequence from the 5' terminal to the 3' terminal; and the long balance joint also includes an intermediate base sequence. The joint can thoroughly solve the problem of data pollution caused by duplication, non-specific annealing and other reasons generated in the PCR process, and overcomes the problems of transcriptome library construction by using SMART technology product lines and base imbalance duringLane sequencing. The invention also provides a method for quickly constructing the transcriptome library by using the joint and adding an internal reference standard substance, so that the steps aresimplified, and the library construction time is shortened to a great extent; and the internal reference standard substance is added, so that the deviation caused by a data result can be subjected tostandardized correction, and the reliability of subsequent analysis is ensured.

The invention provides a kit for miRNA detection and application thereof. The kit comprises a miRNA detection primer, DNA polymerase, incising endonuclease and a digital microfluidic chip. The kit isdeveloped based on the digital microfluidic chip and an isothermal amplification technology, and has relatively high detection sensitivity, specificity and accuracy. At the same time, the kit has relatively strong universality, and the detection of all miRNA can be realized only by designing a specific base sequence of a primer according to specific sequences of different miRNA. The kit provided by the invention directly can be used for directly detecting free and trace miRNA in body fluid without complicated pretreatment, RNA extraction, reverse transcription and the like on a human body fluid sample, is not interfered by other similar miRNA sequences, can realize absolute quantification of miRNA, has the advantages of simple operation, low requirement on detection equipment, low cost andthe like, and is suitable for wide popularization and application.