Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sperm pirna and sperm protein mitopld as biomarkers for detection and prediction of male infertility

A technology of sperm biology and markers, applied in the biological field, can solve problems such as retrotransposon demethylation, de-inhibition of primary piRNA barriers, reproductive barriers, etc., to reduce pain, improve accuracy, and facilitate sample processing and detection Effect

Active Publication Date: 2020-04-24
南京碳硅人工智能生物医药技术研究院有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another protein associated with the formation of primary piRNA is the single-strand specific endonuclease Zuc (Zucchini or MitoPLD) (16-18), mutations in this protein can lead to demethylation of retrotransposons, derepression and primary piRNA production disorders, which eventually lead to reproductive disorders

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sperm pirna and sperm protein mitopld as biomarkers for detection and prediction of male infertility
  • Sperm pirna and sperm protein mitopld as biomarkers for detection and prediction of male infertility
  • Sperm pirna and sperm protein mitopld as biomarkers for detection and prediction of male infertility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: High-throughput next-generation sequencing screening of piRNAs with specific changes as biomarkers of male sperm motility

[0047] (1) The subjects of the study were infertile persons who did not take any contraceptive measures within 2 years after marriage. Age-matched males who had given birth for less than 2 years were used as normal controls. Semen was collected from all subjects after 3 to 5 days of abstinence. WLJY -9000 Weili color sperm quality detection system (Beijing Weili Company) for sperm quality and function analysis. The analysis standards were carried out according to the World Health Organization (WHO Laboratory Manual for Human Semen Examination and Processing (5th Edition)). After semen analysis, it was centrifuged at 1000 g for 10 minutes to collect sperm.

[0048] (2) Collect the sperm samples of 10 cases of normal fertile persons and 10 cases of weak sperm respectively, and mix the samples in the groups respectively. The RNA in the mi...

Embodiment 2

[0052] Example 2: Screening out 10 piRNAs with significant differences from the sequencing results

[0053] According to the two principles of the level of sperm content and the magnitude of the change, the screening conditions were set up: the top 10 piRNAs with the highest content in sperm, and in asthenospermia were reduced by more than 1.5 times compared with the normal control. Based on this screening condition, the following 10 piRNAs were screened out: piR-hsa-28131, piR-hsa-1207, piR-hsa-23317, piR-hsa-27493, piR-hsa-2107, piR-hsa-25783, piR- hsa-2106, piR-hsa-25781, piR-hsa-18709, piR-hsa-25780; the reduction range is shown in the table below, figure 2 b.

[0054]

Embodiment 3

[0055] Example 3: Determination of piRNA expression and specificity changes in seminal plasma by TaqMan probe Real-time PCR method piRNA as a biomarker of sperm motility

[0056] For the two piRNAs of piR-hsa-1207 and piR-hsa-2107, the TaqMan probe Real-time PCR quantitative detection of a single sample was carried out, and RNU6-6P was used as an internal reference; and the specific change of piRNA was further determined as the indicator of sperm motility Biomarkers.

[0057] The specific steps are: extract the total RNA in the sperm of a single sample. For each piRNA, design a specific reverse primer containing the same stem-loop structure, use the piRNA-specific reverse primer for reverse transcription, and obtain a cDNA that contains a common stem-loop structure but belongs to a specific piRNA. The Real-time PCR reaction based on TaqMan probe was carried out, each piRNA was amplified and the fluorescent signal was recorded, and the instrument used was a Roche 480 fluoresce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sperm piRNA and a sperm protein MitoPLD as biomarkers for detecting and predicting male infertility. The biomarkers include 10 piRNAs (mainly piR-1207 and piR-2107) and the MitoPLD protein. The piRNA combination and the MitoPLD protein can be used for detection and prediction of sperm motility. According to the sperm piRNA and the sperm protein MitoPLD as the biomarkers for detecting and predicting the male infertility, spermatozoa are more easily obtained without other tissues, and the sperm piRNA and the sperm protein MitoPLD as the biomarkers for detecting and predicting the male infertility belong to non-invasive examination; the sperm piRNA and the MitoPLD protein can reflect pathological and physiological conditions throughout spermatogenesis at a molecularlevel, and are used as an auxiliary detection index to improve accuracy of detection, and provide potential targets for treatment of male reproductive dysfunction.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to sperm piRNA and sperm protein MitoPLD as biomarkers for detecting and predicting male infertility. Background technique [0002] Infertility has become a worldwide reproductive health problem. Currently, more than 15% of couples in the world suffer from infertility problems (1,2). Among them, about 20% to 30% of infertility is caused by male factors alone, and 50% of infertility is related to male factors. In recent years, this data has been on the rise (2,3), so male infertility Infertility plays an important role, however, the molecular mechanism of male infertility is not fully understood (4,5). At present, the clinical reproductive laboratory inspection items generally include biochemical inspection of semen, acid phosphatase, lactate dehydrogenase-X and other enzyme inspections, and semen immunological inspection, but these technologies have some defects and cannot directly refl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178G01N33/689G01N2800/367
Inventor 陈熹洪叶挺付正夏玉贵
Owner 南京碳硅人工智能生物医药技术研究院有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com