Gene polymorphism sites related to thyroid cancer and application thereof

A thyroid nodule and gene technology, applied in the field of biomedicine, can solve the problems that the technical level is greatly affected, and the benign and malignant thyroid nodules cannot be determined.

Pending Publication Date: 2017-09-15
上海安甲生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even in the best laboratory for pathological diagnosis of thyroid cells in the world, there are still 20-40% of thyroid nodules whose benign and malignant punctures are successful, that is, nodules that cannot be diagnosed by pathology
Therefore, when patients undergo thyroid biopsy, the proportion of patients who cannot be correctly diagnosed due to puncture failure and pathological inability to judge benign and malignant is as high as 30-50%, which is affected by the technical level of thyroid pathologists and puncture physicians in each center bigger

Method used

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  • Gene polymorphism sites related to thyroid cancer and application thereof
  • Gene polymorphism sites related to thyroid cancer and application thereof
  • Gene polymorphism sites related to thyroid cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0210] Example 1 Screening and identification of gene polymorphism sites related to benign and malignant thyroid nodules

[0211] Sample Description: Thyroid fine needle aspiration cells, RNA extraction, and reverse transcription to obtain a cDNA library.

[0212] Specific implementation steps:

[0213] Step (1): According to the possible thyroid cancer-causing genes, including mutant genes and fusion genes, and the gene sequence in GRCh37 / hg19 in UCSC Genome Browser, the chromosome position, coding, gene size, and pseudogene status of the gene were determined.

[0214] Step (2): According to the requirements of Access ArrayTMSystem amplification (the target sequence size is 240bp, there is overlap), design primers, and add tag sequences.

[0215] Step (3): After the primers are synthesized, dilute them, and prepare primer mixtures according to the amplification requirements of the Access ArrayTM System, and divide them into 96-well plates.

[0216] Step (4): Preparation of ...

Embodiment 2

[0243] Preparation and effect verification of embodiment 2 kit

[0244] This embodiment provides a kit for detecting benign and malignant thyroid nodules.

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Abstract

The invention provides somatic mutation sites of pathogenic genes of the thyroid cancer and application thereof, specifically to a group of mutation sites of the pathogenic genes of the thyroid cancer. The mutation sites are composed of a GNAS gene mutation site, a NRAS gene mutation site, a TSHR gene mutation site, etc. The gene mutation sites provided by the invention can be used as markers for identification of benign and malignant thyroid nodules.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular, the invention relates to gene somatic cell-specific mutation sites related to thyroid nodules and applications thereof. Background technique [0002] Thyroid nodules are the most common clinical thyroid disease, and the prevalence of palpable thyroid nodules is about 4-7% in the American adult population. The application of sensitive thyroid B examination, in people over 65 years old, the prevalence of thyroid nodules is as high as 50%. In 2010, Professor Teng Weiping from the Endocrine Association of the Chinese Medical Association conducted an epidemiological investigation of thyroid disease among 15,181 residents in ten cities across the country. It was found that the prevalence of thyroid nodules in the Chinese population was as high as 18.6%, so it is speculated that our country's There are as many as 200 million patients with thyroid nodules. Most thyroid nodules are benign and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/156G01N33/57407
Inventor 宋怀光刘磊
Owner 上海安甲生物科技有限公司
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