Serum protein marker for diagnosing depression and application thereof

A serum protein and protein technology, applied in the field of biological science, can solve the problems of strong subjectivity, high misdiagnosis rate, poor diagnostic consistency, etc., and achieve the effect of good MDD screening value.

Pending Publication Date: 2020-08-18
SOUTHEAST UNIV
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Problems solved by technology

The current clinical diagnosis of mental diseases such as MDD mainly refers to "Chinese Classification and Diagnostic Criteria of Mental Disorders (Third Edition)" (CCMD-3), the United States' "International Classification of Diseases Tenth Edition" (ICD-10), "Mental Disorders" Diagnostic and Statistical Manual of Diseases, Fourth Edition (DSM-IV) and Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5), but the method itself has serious shortcomings: strong subjectivity , poor diagnostic consistency an

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  • Serum protein marker for diagnosing depression and application thereof
  • Serum protein marker for diagnosing depression and application thereof
  • Serum protein marker for diagnosing depression and application thereof

Examples

Experimental program
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[0041] Example 1

[0042] 1. Detection of serum cortisol concentration

[0043] Prepare reagents and samples:

[0044] (1) Put all the reagents and samples in the cortisol kit at room temperature;

[0045] (2) Dilute the serum sample 20 times with Diluent RD5-43, and dilute the 25 times concentrated lotion with distilled water 25 times for use;

[0046] (3) Use diluent RD5-43 to dilute the cortisol protein standard to 10ng / mL, 5ng / mL, 2.5ng / mL, 1.25ng / mL, 0.625ng / mL, 0.313ng / mL, 0.156ng / mL . Add 100μl each of the above-mentioned concentration standards to a row of ELISA plate wells that have been pre-coated with goat anti-mouse polyclonal antibody, and set a blank comparison hole (only diluent RD5-43 is added to the blank comparison hole, No standard is added, that is, the concentration of the standard is 0). The 10ng / mL standard was used as the highest standard, and the diluent RD5-43 was used as a blank control for the 0 standard (B 0 ).

[0047] Experimental steps:

[0048] (1) Pre...

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Abstract

The invention discloses a serum protein marker. The marker is one or more of BDNF, cortisol and/or IFN-gamma protein. The invention also discloses application of the reagent of the serum protein marker in preparation of a mental disease diagnosis product. The invention also discloses an enzyme-linked immunosorbent assay kit. When the three proteins are used together to distinguish MDD and HC, AUCunder an ROC curve is 0.884, the sensitivity and specificity are 86.7% and 83.3% respectively, and the kit has a very good MDD diagnosis value; when only BDNF and cortisol are used in a combined modeto distinguish MDD and HC, AUC is 0.841, specificity is 66.7%, but sensitivity is as high as 90%, and therefore the marker has a good MDD screening value.

Description

Technical field [0001] The invention relates to the field of biological sciences, in particular to a serum protein marker for diagnosing depression and its application. Background technique [0002] Depression (MDD), also known as melancholia, is a type of affective disorder characterized by depression. The WHO reports that MDD will become the world's second-most disease in 2020, and will become the leading cause of death and disability by 2030. The current clinical diagnosis of MDD and other mental diseases mainly refers to the "Chinese Classification and Diagnostic Criteria for Mental Disorders (Third Edition)" (CCMD-3), the United States' International Classification of Diseases Tenth Edition (ICD-10), and The Diagnostic and Statistical Manual of Diseases (Fourth Edition) (DSM-IV) and the Diagnostic and Statistical Manual of Mental Disorders (Fifth Edition) (DSM-5) standards, but the method itself has serious shortcomings: strong subjectivity , The diagnosis consistency is p...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/68G01N33/577
CPCG01N33/743G01N33/74G01N33/6866G01N33/6893G01N33/577G01N2333/57G01N2333/475G01N2800/304
Inventor 袁勇贵陈素珍
Owner SOUTHEAST UNIV
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