The present application provides a method, device, equipment and storage medium for constructing a tumor gene co-expression network. The method includes: acquiring the cancerous transcriptome sequencing data of various tissues in the target human body system and the normal transcriptome sequencing data of the various tissues; the target human body system is any one of the eight major human body systems of human anatomy ; According to the relationship between genes and transcripts, as well as RNAs with uniform gene expression in normal transcriptome sequencing data, differential mRNAs, lncRNAs and miRNAs are extracted from cancer transcriptome sequencing data of various tissues; Carcinogenic differential mRNA, lncRNA, and miRNA of various tissues were analyzed by GO, and a target expression matrix was obtained; the target expression matrix was analyzed by WGCNA, expression modules were constructed according to traits, and the most relevant modules were selected for network construction. It can realize network construction for various cancers.