Method for screening quantitative character candidate genes

A technology for candidate genes and quantitative traits, applied in the fields of genomics, measurement devices, material separation, etc., can solve the problems of increased workload, false positive ratio, and high noise in co-expression analysis of candidate gene identification, so as to speed up the screening process, The effect of noise reduction and efficient screening

Active Publication Date: 2019-05-31
SOUTHWEST UNIV
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Problems solved by technology

However, when these methods are used, the genes or loci screened from the whole genome are associated with known genes, but are indirectly associated with traits, resulting in high noise through co-expression analysis, increasing the proportion of false positives, and laying a foundation for later Candidate gene identification for

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  • Method for screening quantitative character candidate genes
  • Method for screening quantitative character candidate genes
  • Method for screening quantitative character candidate genes

Examples

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Embodiment 1

[0023] A method for rapidly screening candidate genes for quantitative traits of rapeseed oil is constructed through the following process:

[0024] 1. Natural population resequencing for GWAS analysis of oil content and fatty acid content

[0025] In this example, taking 157 materials with wide genetic sources and large differences in seed oil content and fatty acid content as examples, a natural population was constructed, and the method for obtaining candidate genes related to seed oil content was described in detail, as follows:

[0026] (1) Population planting and phenotype acquisition

[0027] Taking 157 materials with different genetic sources, seed oil content and fatty acid content as research materials, they were continuously planted in the field for 5 years (2012-2016). Each material was planted in 3 rows (24 plants in total), and randomized block design. The sowing period of Beibei in Chongqing is mid-to-late September, and field management is carried out by conv...

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Abstract

The invention belongs to the field of molecular breeding, and mainly relates to a method for screening quantitative character candidate genes. The method includes the steps: extracting genes of quantitative character related intervals (QTL interval and GWAS interval) as candidate gene sets; collecting known genes related to the characters as bait genes; carrying out gene co-expression analysis orweight co-expression analysis on the transcriptome data of a set of samples; constructing a co-expression network; and selecting genes with strong connectivity to the bait genes as candidate genes. Compared with previous analysis methods, the method for screening quantitative character candidate genes can quickly and effectively screen candidate genes in the relevant range of quantitative characters, can narrow the screening range of candidate genes, can predict the interaction between genes, can overcome the defect that traditional fine positioning is large in workload and cumbersome, and canaccelerate the molecular breeding process of species.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and mainly relates to a method for screening candidate genes of quantitative traits. Background technique [0002] Most plant traits are complex quantitative traits controlled by multiple genes, such as oil content and plant height. Usually, by constructing segregated populations, molecular markers are used to construct linkage maps, and traits are located, or high-density molecular markers are used to analyze natural populations, and association analysis is carried out to find loci related to traits. Then further build a high-generation backcross population, and perform fine mapping and gene cloning on these sites. This process is not only time-consuming and labor-intensive, but also often increases the difficulty of signing and cloning genes because of the large positioning interval. [0003] The development of genome sequencing technology has enabled a large amount of gene expression data t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/20G16B30/00G16B40/10G01N21/3563G01N21/359G01N30/02G01N33/00G01N33/48
Inventor 钱伟崔艺馨万华方贺亚军熊清丁一娟梅家琴刘智曾晓廖泾行索朗贡布
Owner SOUTHWEST UNIV
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