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Method for predicting unknown corn gene functions on the basis of oil content-related genes and oil content dynamic correlation

A technology of gene function and oil content, applied in the field of plant molecular biotechnology and genetic engineering

Inactive Publication Date: 2019-05-28
UNIV OF JINAN
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Problems solved by technology

However, there are still few reports on methods for predicting the function of unknown genes in maize based on the dynamic correlation between oil content-related genes and oil content.

Method used

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  • Method for predicting unknown corn gene functions on the basis of oil content-related genes and oil content dynamic correlation
  • Method for predicting unknown corn gene functions on the basis of oil content-related genes and oil content dynamic correlation
  • Method for predicting unknown corn gene functions on the basis of oil content-related genes and oil content dynamic correlation

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Embodiment 1

[0034] A method for predicting the function of unknown maize genes based on the dynamic correlation between oil-related genes and oil content mainly includes three steps: collection of oil gene expression data, LA significance assessment and genome-wide LA analysis.

[0035] (1) Collection of gene expression data:

[0036]368 inbred lines were planted in Jingzhou, Hubei in 2010. According to the pedigree information, they were divided into 2 groups (tropical, subtropical, and temperate). In the group, a completely random block method was used, and 2 replicates were set up. Each inbred line had 2 replicates. Sow 1 row. All materials were self-crossed, immature grains 15 days after pollination (15 DAP) were harvested, 3-4 ears were taken from two replicates of each inbred line, 1-2 grains were taken from each ear, and grains were mixed and extracted For total RNA, 368 samples were randomly selected for RNA-seq. The RNA-Seq work of the sample was completed by Shenzhen Genomics ...

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Abstract

The invention belongs to the field of plant molecular biotechnology and genetic engineering and relates to a method for predicting unknown corn gene functions, in particular to a method for predictingunknown corn gene functions on the basis of oil content-related genes and oil content dynamic correlation. The method comprises the following steps: firstly, collecting the grain transcript sequencing 15 days after pollination of a corn selfing line to obtain gene expression quantity data; collecting gene data associated with the oil content of the corn kernels; collecting grain oil content dataof an associated group formed by the corn selfing line; establishing a dynamic association analysis LA model; carrying out LA significance evaluation; excavating a gene for regulating and controllingthe dynamic correlation between the oil content associated gene and the oil content within the whole genome range; and performing function annotation on the gene with the obvious LA result, and predicting the function of an unknown gene. According to the method, the phenomenon that genes in corn kernels are dynamically associated with a co-expression mode is taken as a breakthrough. The function of an unknown gene is predicted. Compared with traditional co-expression network construction, the regulation gene for regulating the co-expression mode can be quickly found through dynamic correlationanalysis.

Description

technical field [0001] The invention belongs to the fields of plant molecular biotechnology and genetic engineering, and in particular relates to a method for predicting the function of unknown corn genes based on the dynamic correlation between oil-related genes and oil content. Background technique [0002] Corn is one of the three major crops in the world. Since the 1990s, the world's total corn production surpassed rice and wheat for the first time, becoming the first food crop. A large amount of storage material including starch, oil and protein accumulates in the corn kernel. With the improvement of people's living standards and changes in dietary structure, as well as the development of starch and oil processing industries, corn varieties have gradually changed from yield-oriented to quality-oriented, and the quality and specificity of corn have become more and more important. [0003] Corn oil is rich in various trace elements such as vitamin E, and the content of u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B25/10C12Q1/6869
Inventor 李慧高幸幸车荣会李为珺
Owner UNIV OF JINAN
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