Rapid maize breeding and population improvement method
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A corn breeding and population technology, applied in the field of breeding, can solve the problems of inaccurate character identification, difficult phenotype identification, long cycle, etc., and achieve the effects of controllable genetic diversity, improved breeding efficiency, and accurate phenotype.
Active Publication Date: 2019-03-29
INST OF CROP SCI CHINESE ACAD OF AGRI SCI +1
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[0004]
The following problems exist in the existing population improvement technology: 1) The cycle is long, and it often takes 5-8 generations; 2) It is difficult to identify the phenotype. The existing technology is often to identify the heterozygous population. , character identificati...
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Embodiment 1
[0023] 1. According to the breeding objectives, breeding background, and heterosis group types, the basic comprehensive population can be constructed. It can be formed by mixed pollination of 2-5 corn parental inbred lines, or can be directly formed by self-separation of hybrids. This embodiment adopts the following Those, specifically the F of the hybrid Zhongdan 909 2 Generation separation population to build basic population (P 0 ).
[0024] 2. Use 55K high-density (more than 10,000 is better, 55K) SNP molecular markers are used for marker analysis on parental materials. The markers used come from 55K corn chips (Xu C., Ren Y., Jian Y., Guo Z. , Zhang Y., Xie C., Fu J., Wang H., Wang G., Xu Y., Li P., Zou C. Development of a maize 55K SNP array with improved genome coverage for molecular breeding. Molecular Breeding, 2017 , 37:20).
[0025] 3. For the basic group P 0 Perform haploid induction and double to generate 150 DH lines (D 0 group).
[0026] 4. To D 0 The pop...
Embodiment 2
[0042] Carry out the test according to the same method of embodiment 1 step 1-5.
[0043] 6. Use the linkage analysis method to excavate 80 loci controlling grain weight markers, see Table 2 for details. The markers are derived from the corn 55K chip (Xu C., Ren Y., Jian Y., Guo Z., Zhang Y., Xie C ., Fu J., Wang H., Wang G., XuY., Li P., Zou C. Development of a maize 55K SNP array with improved genome coverage for molecular breeding. Molecular Breeding, 2017, 37:20).
[0044] Table 2 control grain weight marker sites
[0045]
[0046]
[0047]
[0048]
[0049] 7. Combining phenotype identification, trait linkage site information and high-density molecular detection data, a genome-wide selection model is established for phenotype prediction of offspring populations. In this example, the established genome-wide selection model was used to predict the grain weight phenotypes of 150 DH line populations derived from Zhongdan 909, and the prediction accuracy reached 7...
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Abstract
The invention provides a rapid maize breeding and population improvement method, wherein the method includes the following steps: carrying out genotype identification of a basic population by using ahigh-density SNP molecular marker, combining with field phenotype identification, selecting an excellent individual population, and making the excellent individual population rapidly produce a large number of DH lines by using a double-haploid technology; carrying out multi-point field phenotype identification and combining ability determination on the DH population, selecting individual plants with good performance, carrying out molecular marker identification, and analyzing and mining target trait associated marker loci; establishing a whole-genome selection model; screening a plurality of best DH lines with the established whole-genome selection model, and carrying out mixing pollination to form a new basic population; utilizing the double-haploid technology again to make the basic population rapidly produce a large number of DH lines, carrying out phenotypic prediction screening by the whole-genome selection model, carrying out multi-point field phenotype identification on the screened excellent plants, and screening good selfing lines; and circulating the steps to improve the basic population, and carrying out selected breeding of more good selfing lines.
Description
technical field [0001] The invention relates to breeding technology, in particular to a method for rapid improvement of corn breeding populations. Background technique [0002] Maize breeding is one of the important directions of agricultural research, and cross breeding is an important way of maize breeding. In maize hybrid breeding, the creation of excellent breeding basic materials is the key to the success of breeding. Commonly used population creation methods include population improvement, pedigree selection, backcross selection, etc., but these methods take a long time for breeding, often requiring 6-8 generations (3-5 years), with little genetic gain and low breeding efficiency. The specific problems that exist are as follows: 1. Low-generation group materials (such as S 1 -S 3 ) is a heterozygous state. Due to the presence of dominant-recessive and gene interactions, phenotypic identification is not accurate and cannot fully represent the phenotypic results of th...
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