Preparation method and application of gold-upconversion nanoparticle tripolymer

Abstract

The invention relates to a preparation method and application of a gold-upconversion nanoparticle tripolymer, belonging to the technical field of analytical chemistry. The preparation method mainly comprises the following steps: synthesis of gold nanoparticles with the particle size of 20nm, modification of Mucin-1 aptamer on gold nanoparticles, modification of AFP aptamer on upconversion nanoparticles, modification of AFP and Mucin-1 aptamer partial complementary sequences on gold nanoparticles, assembly of gold-upconversion nanoparticle tripolymer, and fluorescent and Raman detection. After the AFP is applied, the fluorescence can be enhanced; and after the Mucin-1 is applied, the Raman signal becomes weak, thereby establishing the standard curve between fluorescent signals and AFP concentration and the standard curve between Raman signals and Mucin-1 concentration. The invention provides a multiplex supersensitive detection method for cancer biomarkers by using the gold-upconversion nanoparticle tripolymer. Compared with the traditional detection methods, the multiplex supersensitive detection method has the advantages of low cost and high sensitivity, is convenient and quick, and has favorable practical application prospects.

Description

technical field [0001] The invention relates to a preparation method and application of a gold-upconversion nano particle trimer, belonging to the technical field of analytical chemistry. Background technique [0002] Alpha-fetoprotein (α-fetoprotein, AFP) is a carcinogenic glycoprotein and a specific tumor marker for the diagnosis of primary liver cancer. It has the functions of establishing diagnosis, early diagnosis and differential diagnosis. A large number of clinical findings, 70% -95% of patients with primary liver cancer AFP increased. Mucin-1 occurs in many adenocarcinomas, including pancreatic, lung, breast, ovarian, colon and other tissues. Mucins are also overexpressed in lung diseases such as asthma, bronchitis, chronic obstructive pulmonary disease or cystic fibrosis. Mucin-1 is a positive diagnostic marker for malignancy and other disease processes and is often over or misexpressed. [0003] Traditional methods for detecting cancer markers include: enzyme-l...

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Problems solved by technology

When detecting tumor markers by enzyme-linked immunosorbent assay, it is difficult to detect protein tumor markers because proteins are easily thermally denatured. In addition, the sensitivity of enzyme-linked immunosorbent assay is low, and it is labor-intensive and time-consuming; electrochemistry has relatively high requirements for the matrix of samples. High, it is difficult to implement; and the cost of testing with instruments is very high, and there are high requirements for the operator's skills

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