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Ochratoxins upconversion fluorescent sensing material and preparation method thereof

An ochratoxin and sensing material technology, which is applied to the field of ochratoxin up-conversion fluorescent sensing materials and their preparation, can solve the problems of inability to identify targets, lack of specificity of UCNPs, etc., and achieves easy popularization, convenient material preparation, The effect of strong recognition function

Inactive Publication Date: 2016-02-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of up-conversion fluorescent sensing materials in the field of food safety is still seldom, and there is no research on the detection of mycotoxins, and UCNPs lack specificity and cannot target specific recognition targets.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The invention provides a preparation method of ochratoxin up-conversion nano-fluorescence sensing material, the specific preparation method is as follows:

[0024] (1) Weigh 700mg of sodium hydroxide and dissolve it in 5mL of water and 8mL of ethanol, dissolve completely at room temperature, then add 8mL of oleic acid to form a slightly yellow clear solution; add 2.5mL of yttrium nitrate, ytterbium nitrate and nitric acid to the above solution Erbium mixture (0.4molL -1 ), to obtain a milky white congealed solution; after 10min, add 247mgNH 4 20mL of ethanol + water (1+1, v / v) in F was stirred for 10min and ultrasonicated; the solution was transferred to a polytetrafluoroethylene-lined high-temperature reactor, reacted at 220°C for 12h, cooled to room temperature, and centrifuged. , washed with ethanol;

[0025] (2) Weigh 100 mg of the above-prepared up-conversion nanoparticles and dissolve them in 100 mL of TritonX-100+H 2 O(1+4, v / v), react for 8 hours, centrifuge ...

Embodiment 2

[0030] The adsorption performance of the sensing material was studied. Add 1mg of UCNPsMIP or UCNPsNIP into a 4mL centrifuge tube, then add 2mL with concentrations of 0.2, 0.4, 0.6, 0.8, 1.0, 2.0, 2.5, 4.0, 8.0, 10.0mgL -1 ochratoxin standard solution. The mixture was shaken at room temperature for a period of time, and then its fluorescence value was measured with a fluorescence spectrophotometer. With the increase of the concentration of ochratoxin, the fluorescence intensity weakened, and at the same concentration, the fluorescence quenching of UCNPsMIP was greater than that of UCNPsNIP.

[0031] Example 2

[0032] The dynamic performance of the sensing material was studied. Weigh 1 mg of UCNPsMIP prepared in Example 1 and put it in a 4 mL centrifuge tube, then add 2 mL of 1 ppm ochratoxin A, and shake at room temperature for 0.5, 1, 1.5, 2, 2.5, 2, 2.5, 4, 4.5 h, Then use a fluorescence spectrophotometer connected with an external 980nm exciter to measure the fluoresce...

Embodiment 4

[0034] Select Aflatoxin B 1 (AFB 1 ) is a competitor of ochratoxin A (OTA), and the selectivity of the sensing material is studied. Prepare the concentration of 1.0mgL respectively -1 AFB 1 Single standard solution with OTA and mixed solution of both. Take 2mL of the prepared solution and put it into a centrifuge tube, add 1mg of UCNPsMIP and UCNPsNIP respectively, shake at room temperature for 2h, and then measure the fluorescence value with a fluorescence spectrophotometer connected with an external 980nm exciter. It shows that UCNPsMIP has selective recognition for the target toxin. OTA has a significant quenching effect on the fluorescently imprinted polymeric material UCNPsMIP, while the competitor AFB 1The quenching effect on it is relatively weak; OTA and AFB 1 The degree of quenching of fluorescent non-imprinted polymeric material UCNPsNIP is very small. In the presence of AFB1, OTA quenched UCNPsMIP to the same extent as OTA alone. It was verified that UCNPsMI...

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Abstract

The invention provides an ochratoxin upconversion fluorescent sensing material. The invention also discloses a preparation method of the sensing material. The method is as below: (1) adding ochratoxin, functional monomer 3-aminopropyltriethoxysilane into a methanol solution, stirring to fully dissolve and react, then adding the upconversion nanoparticles (UCNPs), stirring for 50 min, then adding tetraethoxysilane and a catalyst acetic acid, and incubating in a water bath at 30 DEG C for 12 h; (2) conducting pumping filtration, washing away unreacted materials by a PBST solution, conducting vacuum drying and aging at 50 DEG C for 10 h; placing the polymer in a Soxhlet extractor, washing away the ochratoxin by using 2% trichloroacetic acid PBST mixed solution as a solvent, and conducting vacuum drying a 50 DEG C to obtain a fluorescent molecular imprinting material with high selectivity for ochratoxins. The invention has the advantages that the molecular imprinting sensing material synthesized by the sol-gel method uniform grain size, high selectivity on ochratoxin, and broad application prospects.

Description

technical field [0001] The invention belongs to the research field of optical sensing material preparation technology and microbial detection method, and in particular relates to an ochratoxin up-conversion fluorescent sensing material and a preparation method thereof. Background technique [0002] Ochratoxins include 7 compounds with similar chemical structures, among which Ochratoxin A (Ochratoxin A, OTA) is the most widely distributed in nature, the most toxic, and has the greatest impact on humans, animals and plants. It often exists in various food, feed and other agricultural and sideline products. Therefore, it is imperative to increase the supervision and inspection of grain, oil and food, and to establish appropriate and feasible detection methods for mycotoxins. [0003] Up-converting fluorescent nanoparticles (UCNPs) are developing rapidly as a new type of luminescent material. Compared with traditional fluorescent materials (such as quantum dots), UCNPs have th...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 方国臻闫祯王俊平王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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