Preparation method and application of composite nano material paper chip electrochemical luminescence immunosensor

Abstract

The invention discloses a method for researching and applying a paper chip electrochemical luminescence immunosensor for detecting tumor marker by using a composite nano material. The paper chip and sensor preparation method (see the schematic diagram in the attached figure) mainly comprises the steps of printing designed channel patterns on a piece of chromatographic paper by using a printer; heating the wax printed chromatographic paper by using an electronic temperature controller; preparing a graphene-metal composite nano material and a quantum dot-porous silicon nano material; modifying the nano material on the paper chip; connecting an antibody to the paper chip modified by the nano material; after antigen specificity identification, carrying out antigen specificity identification on the antibody marked by a luminescent material so as to make the electrochemical luminescence immunosensor; and detecting an electrochemical luminescence signal with the combination of the processed paper chip with a screen-printed electrode. The sensor is strong in specificity detection and high in sensitivity, and the sensitivity can be of ng grade; the diagnosis and monitoring speed is fast, and the time for accomplishing one basic detection process is shorter; and a film electrode at the bottom of the sensor can be repeatedly used for multiple times, so that the cost is low.

Description

technical field [0001] The invention relates to the fields of composite nanomaterial technology, paper chip technology and tumor marker detection technology, and more specifically relates to an electrochemiluminescence immunosensor that uses composite nanomaterials to detect tumor markers by means of a paper chip. Background technique [0002] Tumor is the killer of human health. Malignant tumor, also known as cancer, is a type of disease that seriously threatens human health and life. It is caused by the body being affected by various internal or external carcinogenic factors, and the cells of local tissues lose their normal growth at the genetic level. Regulation, resulting in the formation of new organisms due to abnormal proliferation. Tumor occurrence is a long-term, multi-stage, multi-gene accumulation process, with the complexity of multi-factor regulation and multi-gene control. The rising trend of tumor morbidity and mortality directly threatens human health, and ...

AI Technical Summary

Problems solved by technology

At present, the main immunoassay methods include electrochemical immunoassay, chemiluminescence immunoassay, flow injection chemiluminescence immunoassay, high performance liquid chromatography chemiluminescence immunoassay, capillary electrophoresis chemiluminescence immunoassay, etc. There are still deficiencies, so a new sensitive detection method is urgently needed

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