Method for electrochemically detecting concentration of glucose in whole blood through integrated paper based micro-fluidic apparatus

Abstract

The invention discloses a method for electrochemically detecting the concentration of glucose in whole blood through an integrated paper based micro-fluidic apparatus. The method comprises the following steps: dispersing graphene/polyaniline/nano-gold composite material in a chitosan-acetic acid solution to prepare a graphene/polyaniline/nano-gold composite material mixed solution, uniformly mixing the graphene/polyaniline/nano-gold composite material mixed solution with a glucose oxidase solution to obtain a solution mixture, modifying a screen printing electrode, fixing the modified screen printing electrode, adding a sample to a filter paper disc in a dropwise manner, air-drying, covering the surface of the modified screen printing electrode with the filter paper disk, adding a phosphate buffer solution in a dropwise manner, and carrying out electrochemical test. A small filter paper disk is used as a support in the invention, so convenient sample preservation, reduction of the reagent consumption and the sample consumption, and making cost saving are realized.

Description

technical field [0001] The invention relates to instant electrochemical detection of glucose in whole blood, specifically, based on the direct electrochemical reaction of glucose oxidase modified on a screen-printed electrode and combined with filter paper disc storage reagents to measure the glucose in whole blood. Background technique [0002] Since the first glucose sensor was born in 1967 [see: Wilson, R, Turner, A.P.F., Biosens. Bioelectron., 1992, 7, 165-185.], due to its low cost, commercial utility, high biocatalytic activity and stability Its application range has expanded from clinical laboratories to people's self-testing. It is widely used to monitor the concentration of glucose in the blood of diabetic patients, which is beneficial to the prevention and treatment of diabetes with increasing incidence. [0003] With the development of the third generation of biosensors, that is, modifying the surface of the electrode to construct an interface for direct electron ...

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Problems solved by technology

There have been many reports on the use of direct electrochemical properties of glucose oxidase (GOD) to determine glucose content [see: Wang, Y., Liu, L., Li, M., Biosens. Bioelectron.2011, 30, 107-111 .], but did not see the use of third-generation biosensors to directly measure the report of glucose in whole blood, more is to measure the concentration of glucose in serum, which creates difficulties for the real-time determination of blood glucose concentration

Moreover, the traditional electrolytic cell used in the electrochemical test of the prior art requires a large amount of reagents and samples, and the cost is high, and it is a waste of resources at the same time.

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