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Portable ractopamine molecular imprinted silk-screen printed electrochemical sensor

A ractopamine, screen-printed electrode technology, applied in the field of rapid detection, can solve problems such as hormone residues, affecting consumers' health, and side effects of animal bodies, achieving high selectivity, avoiding instability, and improving stability.

Active Publication Date: 2016-03-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ractopamine belongs to hormone additives, and hormone feed additives have the problem of hormone residues after being used in animals, causing side effects on animal organisms, and also affecting the health of consumers

Method used

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  • Portable ractopamine molecular imprinted silk-screen printed electrochemical sensor
  • Portable ractopamine molecular imprinted silk-screen printed electrochemical sensor
  • Portable ractopamine molecular imprinted silk-screen printed electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of Ractopamine Molecularly Imprinted Screen Printing Electrode

[0041] Schematic diagram of the preparation of ractopamine molecularly imprinted screen-printed electrode sheet as shown in figure 1 shown. The specific preparation method is as follows:

[0042] (1) Modification of mesoporous carbon CMK-3 on the surface of the working screen-printed electrode: the carbon nanomaterial mesoporous carbon CMK-3 was dissolved in dimethylformyl (DMF) amine solution, and prepared into a suspension of 1.0 mg / ml solution, then pipette 10 μL dropwise onto the working electrode, and dry at room temperature.

[0043] (2) Electroplating and depositing gold nanoparticles on the surface of the working screen printing electrode, electroplating method: the working electrode is placed at 2.43mmol L -1 Chlorauric acid and 0.1mol·L -1 In the sulfuric acid solution, under the potential of -0.2V, the electroplating time is 80s to complete the electroplating;

[0044]...

Embodiment 2

[0051] Example 2 Influence of Preparation Conditions of Ractopamine Molecularly Imprinted Screen Printing Electrode on Detection Effect

[0052] (1) Electrochemical behavior characterization of composite modified mesoporous carbon CMK-3 and gold nanoparticles

[0053] In order to improve the stability and sensitivity of the screen-printed bare electrode, the present invention double-modifies the bare electrode with CMK-3 and gold nanoparticles. The electroactive surface area of ​​the bare electrode was greatly increased after the double modification was verified by the CV response of the bare electrode and the modified electrode (as shown in the attached figure 2 ). The peak difference of bare electrode, gold nanoparticle / bare electrode, CMK-3 / bare electrode, CMK-3 / gold nanoparticle / bare electrode and gold nanoparticle / CMK-3 / bare electrode are 390mV, 310mV, 150mV, 95mV respectively and 85mV, indicating that the electron transfer rate was greatly increased on the CMK-3 / gold ...

Embodiment 3

[0057] Example 3 Application of Ractopamine Molecularly Imprinted Screen Printing Electrode

[0058] The structure diagram of the portable ractopamine molecularly imprinted electrochemical detector is as follows: Figure 4 As shown, the structure is as follows: .

[0059] Molecularly imprinted screen printing electrodes include electrode substrates, conductive silver paste, electrode connections, working electrodes, counter electrodes, and insulating layers; the surface of the working electrodes is electroplated with molecularly imprinted polymer films; the electrode substrates are disposable consumer substrates , The working electrode, the counter electrode and the reference electrode are derived from the electrode connection, forming a convex three-wire socket, which can be inserted into a portable electrochemical detector for detection and reading. The electrode substrate is printed with an electrode base body in which connecting terminals, electrode connecting wires and wor...

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Abstract

The invention discloses a portable ractopamine molecular imprinted silk-screen printed electrochemical sensor, and belongs to the technical field of fast detection. 60-100 [mu]L of an electric conductance solution contains a to-be-detected sample is dropwise added to the surface of a ractopamine molecular imprinted silk-screen printed electrode, the standing adsorption time is 50-200 s, then the ractopamine molecular imprinted silk-screen printed electrode is horizontally inserted into a clamping slot of a portable electrochemical detector, a current peak value during scanning is recorded, calculation is performed according to a standard curve between the ractopamine concentration and the current peak value I([mu]A)=-14.944logC([mu]M)+0.2641 (R<2>=0.9923); and finally, the detected concentration of ractopamine in the sample is displayed on an LED screen. The sensor has the advantages of high precision, high stability and high repeatability.

Description

technical field [0001] The invention relates to a portable ractopamine molecularly imprinted screen-printed electrochemical sensor, which belongs to the technical field of rapid detection. Background technique [0002] Ractopamine (RAC) is a synthetic β-adrenergic receptor agonist, which belongs to a kind of "clenbuterol", which can simultaneously improve the daily quality of animals, improve feed utilization, and increase animal protein content. At present, only 24 American and Asia-Pacific regions such as the United States, Canada, and Brazil allow the use of ractopamine for growth promotion and lean meat improvement of food animals (mainly for pigs and cattle). Ractopamine belongs to hormone additives, and hormone feed additives have the problem of hormone residue after being used in animals, which will cause side effects on animal organisms and also affect the health of consumers. The European Union has already clearly banned the use of all adrenaline stimulant drugs in...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 孙秀兰马敏皮付伟纪剑张银志孙嘉笛
Owner JIANGNAN UNIV
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