Application of screen printing electrode in the determination of 5-hydroxytryptamine

A technology of screen printing electrodes and serotonin, applied in the direction of electrochemical variables of materials, can solve the problem of high cost and achieve the effect of high selectivity and sensitivity

Inactive Publication Date: 2010-02-03
武汉思达创新医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many electrodes used in the detection of serotonin, most of which are columnar microele

Method used

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  • Application of screen printing electrode in the determination of 5-hydroxytryptamine
  • Application of screen printing electrode in the determination of 5-hydroxytryptamine
  • Application of screen printing electrode in the determination of 5-hydroxytryptamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: at first choose PVC substrate clean and dry heat-resistant, then print electrode lead wire, then dry substrate, print carbon electrode and counter electrode with the carbon ink that contains colloidal gold particle and dry (colloidal gold content is every ml carbon ink containing 0.01μg colloidal gold), printed Ag / AgCl electrodes and dried, and finally printed the insulating layer with insulating paste and cured. Take 20 μL of 10% nafion solution and coat it on the surface of the carbon electrode, and wait for the solvent to evaporate to obtain a sensitive film.

Embodiment 2

[0030] Embodiment 2: firstly select the substrate to clean and dry heat-resistant, then print the electrode leads, then dry the substrate, print carbon electrodes and counter electrodes with the carbon ink containing colloidal gold particles and dry, (colloidal gold content is per ml carbon ink containing 100 μg colloidal gold) to print the Ag / AgCl electrode and dry it, and finally use the insulating paste to print the insulating layer and cure it. Take 0.1 μL of 0.2% nafion solution and coat it on the surface of the carbon electrode, and evaporate the solvent to obtain a sensitive film.

Embodiment 3

[0031] Example 3: First select the substrate to clean and dry heat-resistant, then print electrode leads, then dry the substrate, print carbon electrodes and counter electrodes and dry, print Ag / AgCl electrodes and dry, and finally use insulating paste The insulating layer is printed and cured.

[0032] 80 KM mice were divided into four groups of high reserpine (2 mg / kg), medium (0.5 mg / kg), low (0.25 mg / kg) and blank, 20 in each group. After intraperitoneal injection of reserpine injection, 4 mice were taken from each cage at 0, 2, 3, 5, and 9 hours respectively, and 100 μL of blood was taken, and diluted with 0.05 mol / L phosphate buffer (pH 7.0) 5 times, for the test solution 1. The brain was decapitated and homogenized with 1ml of 0.05mol / L phosphate buffer (pH 7.0). After the protein was measured, the volume was adjusted with the above buffer, which was the test solution 2. And at the same time, their depressive behavior was scored.

[0033] Insert the electrode made in...

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PUM

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Abstract

The invention relates to the application of a screen printing electrode in the determination of 5-hydroxytryptamine. The invention has higher sensitivity and low cost by using the screen printing electrode for determining 5-hydroxytryptamine. The screen printing electrode comprises an electrode substrate which is printed with a carbon electrode, a counter electrode and an Ag/AgCI electrode; each electrode is correspondingly connected with an electrode lead wire. Carbon printing ink manufacturing the carbon electrode can contain aerosol granules so as to increase the response sensitivity of electroactive material.

Description

technical field [0001] The invention relates to a screen-printed electrode and its application in measuring the content of 5-hydroxytryptamine (5-HT) in biological samples. Background technique [0002] 5-Hydroxytryptamine is an important neurotransmitter in the human body, which is involved in many life disease processes. Studies have shown that the occurrence of depression is closely related to the level of 5-HT. At present, the diagnosis of clinical depression is mainly based on the patient's medical history and clinical manifestations, through scales or psychological diagnosis, such as the Hamilton Depression Rating Scale (HAMD), its accuracy is not high, and it is easy to misdiagnose. It is of great medical significance to find a fast and accurate 5-HT assay method that can realize rapid clinical testing. The current 5-HT concentration determination method is mainly high-performance liquid chromatography, which is cumbersome, requires a large amount of samples, and tak...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 丁虹刘敏
Owner 武汉思达创新医药科技有限公司
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