Antibiotic residue detector based on screen-printed electrode aptamer sensor

A technology of screen printing electrodes and aptamer sensors, applied in the direction of electrochemical variables of materials, etc., can solve the problems of weak signal of immunosensors, unsatisfactory reproducibility, difficult to prepare antibodies, etc., to improve sensitivity, promote electron transfer, The effect of avoiding cross-interference problems

Inactive Publication Date: 2015-09-16
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Antibiotics are small molecule hapten substances, and it is difficult to prepare corresponding antibodies. Even if corresponding antibodies are prepared, there are still problems such as weak signal of immune sensor, poor reusability, and reproducibility that cannot meet the requirements of actual sample detection.

Method used

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  • Antibiotic residue detector based on screen-printed electrode aptamer sensor
  • Antibiotic residue detector based on screen-printed electrode aptamer sensor
  • Antibiotic residue detector based on screen-printed electrode aptamer sensor

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Embodiment Construction

[0026] Below in conjunction with accompanying drawing and embodiment the technical solution of the present invention is described in detail, figure 1 It is a structural block diagram of an aptamer sensor antibiotic residue detector based on a screen-printed electrode of the present invention. The present invention is composed of a detection circuit, a screen-printed electrode aptamer sensor, a power supply, buttons, a microcontroller, and a display screen , wherein the detection circuit is composed of a power supply circuit, a detection chip, a code switch, a calibration resistor, an alligator clip signal input line, and a screen-printed electrode aptamer sensor; the detection circuit is used to generate an excitation voltage and apply it to the immune sensor, and then sample The response signal after excitation is digitally processed, and the result is sent to the microcontroller; the microcontroller and the detection program running on it process the input signal again, and ...

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Abstract

The invention relates to an antibiotic residue detector based on a screen-printed electrode aptamer sensor. The detector is composed of a detection circuit, a screen-printed electrode aptamer sensor, a power supply, keys, a microcontroller and a display screen, wherein a detection chip generates excitation voltage which is applied to the screen-printed electrode aptamer sensor to collect corresponding impedance signals. Ions and ferroferric oxide are used for modifying the surface of a screen-printed electrode layer by layer, then the screen-printed electrode stands still at room temperature till getting dry, and tetracycline aptamer is fixed on the surface of the screen-printed electrode which is modified by a nanometer material, and thus the screen-printed electrode aptamer sensor is prepared. The antibiotic residue detector can realize on-line and rapid detection for antibiotic residue, has the characteristics of strong practicability, convenience in carrying, high detection accuracy, rapidness and accuracy in detection, simplicity in operation and the like, and is particularly suitable for rapidly detecting antibiotic residue on site.

Description

technical field [0001] The invention relates to an antibiotic residue detector, in particular to an antibiotic residue detector based on an aptamer sensor of a screen-printed electrode, which integrates a biosensor detection technology and an embedded system design method for convenient, rapid, Accurately detect what kind of antibiotic residues are contained in milk and other agricultural products and whether they exceed the standard. The invention belongs to the technical field of agricultural product safety detection. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry industry, milk production has increased significantly, and fresh milk and dairy products have become an important part of people's (especially the elderly and children's) daily food. Antibiotics are widely used to prevent and treat dairy cow diseases. Due to frequent and overdose use, there are a certain amount of antibiotic residues in milk and other a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 郭业民孙霞焦艳翠王相友
Owner SHANDONG UNIV OF TECH
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