Construction method of visual self-energized glucose biosensor

Abstract

The invention discloses a visual portable three-dimensional hollow passageway microfluidic paper chip sensor which is simple in operation, low in cost and self-energized, and is successfully used for field inspection. A biological fuel cell is successfully introduced into a three-dimensional hollow passageway microfluidic paper chip by virtue of the sensor; by virtue of the negative and positive electrodes of the biological fuel cell, an electrical signal for the catalytic effect of a substrate is automatically generated, and restriction on energy supply equipment is avoided. The construction process of the sensor is as follows: largely printing lyophobic patterns, carrying out molten wax molding, and preparing hollow passageway by use of a laser cutting machine, then printing a screen-printed electrode, and preparing electrodes of the biological fuel cell by virtue of long gold; preparing a complex three-dimensional graphene modified electrode; respectively modifying the positive and negative electrodes of the biological fuel cell with a glucose oxidase and a laccase; and synthetizing an electrochromic material and uniformly painting the electrochromic material on the ITO, clamping each paper chip unit by virtue of a self-control circuit board, connecting the paper chip units with the ITO painted with the electrochromic material, adding a glucose solution to trigger reaction, and determining the glucose concentration range by virtue of observing color changes of the electrochromic material.

Description

technical field [0001] The invention relates to the field of semi-quantitative analysis and detection of glucose. A portable visual self-powered glucose biosensor is constructed. A biofuel cell is constructed by using a hollow channel microfluidic paper chip as a substrate. Glucose is used as the fuel of the biofuel cell, combined with electrochromism Technology realizes the visual detection of glucose. Background technique [0002] Glucose is an indispensable substance in life activities, and it can directly participate in the metabolic process in the human body. Glucose is an important substance in living organisms, and its content in body fluids is an important indicator reflecting the state of the body. Especially for diabetic patients, the determination of blood glucose concentration is an important means to control the disease, so it is extremely important to accurately and quickly determine the content of glucose in blood. For a long time, with the continuous effort...

AI Technical Summary

Problems solved by technology

However, the above-mentioned methods are expensive, complicated to operate, and have a strong dependence on energy, which is not conducive to on-site detection anytime and anywhere, which limits its current application and development.

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