Method for improving surface biological activity of carbon fiber reinforced polyether-ether-ketone composite material
A polyetheretherketone, bioactive technology, applied in the field of medical biocomposite materials, can solve the problems of poor biological activity, poor osteogenic integration ability, etc., and achieve the effects of low cost, improved biological activity, and simple process method.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] According to the method of the invention, the surface modification experiment of carbon fiber reinforced polyetheretherketone composite material is carried out.
[0027] (1) Cut the carbon fiber reinforced polyether ether ketone sheet into discs with a diameter of 14 mm and a thickness of 2 mm.
[0028] (2) The cut carbon fiber reinforced polyether ether ketone wafer was ultrasonically cleaned three times in sequence with acetone, ethanol and distilled water respectively, and after each time for 30 min, it was dried in a vacuum oven at 60°C and stored for later use. The obtained sample was named un-CFRPEEK.
[0029] (3) The cleaned and dried carbon fiber reinforced polyetheretherketone disc was immersed in concentrated sulfuric acid, and reacted on a magnetic stirrer for 5 minutes at a rotation speed of 500 rpm / min. After sulfonation reaction at room temperature for 5 minutes, the disc was quickly taken out and placed in distilled water to terminate the reaction, and t...
Embodiment 2
[0041] Dexamethasone (Dex) was selected to evaluate the drug sustained release ability of the carbon fiber reinforced polyether ether ketone S-CFRPEEK@GelMA / PAAM obtained in Example 1 above after sulfonation treatment and UV grafting treatment.
[0042] The specific drug sustained release experimental steps of CFRPEEK@GelMA / PAAM are as follows:
[0043] (1) First use a UV spectrophotometer to measure the standard curve of dexamethasone at 243nm.
[0044] (2) Soak the UV-treated S-CFRPEEK@GelMA / PAAM in 250 μg / ml dexamethasone solution, and use the adsorption capacity of the hydrogel to absorb the drug. After soaking for 24 hours, use an ultraviolet spectrophotometer to measure the concentration of the remaining dexamethasone solution at 243nm after drug inhalation. The drug loading of S-CFRPEEK@GelMA / PAAM was calculated according to the concentration difference before and after drug inhalation.
[0045] (3) Transfer the absorbed S-CFRPEEK@GelMA / PAAM to a 10ml centrifuge tube,...
Embodiment 3
[0052] Rat bone marrow mesenchymal stem cells (rBMSCs) were cultured in vitro to evaluate the cell activity of the carbon fiber-reinforced polyetheretherketone material obtained through the surface modification treatment in Example 1 above. The Cell Counting Kit (CKK-8, Beyotime, Shanghai, China) kit was used to detect the proliferation of cells on the surface of the material. The specific operation method is as follows:
[0053] Put the sample sterilized by Cobalt 60 into a 24-well culture plate, and the density of each well is 1×104cell / ml cell suspension.
[0054] Place the cell culture plate in a cell culture incubator with 5% CO2 saturated humidity at 37°C, and change the culture medium every 2-3 days.
[0055] After 1, 4 and 7 days of cell culture, the original culture solution was sucked off, and 200 μl of new culture solution containing 10% CKK-8 solution was added. After the culture plate was placed in the incubator for 2 hours, 100 μl of the culture solution was tak...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com