Preparation method of novel neural restoration tissue engineering scaffold

Abstract

The invention relates to the technical field of biological composite materials, and discloses a preparation method of a novel neural restoration tissue engineering scaffold. The preparation method comprises the following steps: modifying bacterial cellulose; preparing a living cell derived material; uniformly mixing the modified bacterial cellulose with the living cell derived material to obtain a mixture, injecting the mixture into a mold, and preparing the neural restoration tissue engineering scaffold by an ice crystals directional solidification method and a freeze vacuum drying method. The preparation method has the benefits that due to dual bionic design of a structure and a component, the living cell derived material, modified nano fibers and a conductive nano material are combined, so that the obtained scaffold is high in moisture content, good in comprehensive mechanical property and high in stability, and has bioactivity and electroactivity; meanwhile, growth and increment of blood cells can be promoted favorably, and a function of axonal regeneration can be guided; the preparation method is simple, and the shape and the size of a prepared product are easy to control; various requirements of the neural restoration tissue engineering scaffold can be met, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of biological composite materials, in particular to a preparation method of a novel nerve repair tissue engineering scaffold. Background technique [0002] Peripheral nerve defects and spinal cord injuries have always been difficult in neuroscience research. Peripheral nerve injuries account for 1.5% to 4.0% of the newly increased trauma cases worldwide each year, and a large number of patients need nerve repair. The reconstruction of the regenerated nerve fiber structure depends on many factors, including whether the proximal axonal buds grow vigorously; whether the "paving" of Schwann cells is complete; whether the properties of the regenerated nerve fibers are compatible with the target organ. One must. Although peripheral nerve repair has a history of more than 150 years, its repair results have been very limited. With the progress of neuroscience and tissue engineering research, people realize that th...

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Problems solved by technology

[0003] At present, there are three types of scaffold materials for nerve repair: 1. Natural scaffold materials; mainly autologous or allogeneic nerves, veins, skeletal muscle, and amniotic membrane, etc., but the above materials are difficult to obtain due to their limited sources, and there is a risk of cross-transmission of pathogenic microorganisms , difficult to mass-produce

2. Natural biomaterials: mainly collagen, gelatin, chitosan, alginate, cellulose, hyaluronic acid, aminodextran, liposome, decellularized extracellular matrix, etc. The advantages of these materials are mainly reflected in biological Good compatibility, low antigenicity, some materials have a natural pore structure system, which is conducive to cell adhesion, growth and proliferation, but the mechanical properties are poor and lack sufficient physical strength

However, most synthetic materials lack biological activity, do not promote tissue recovery and reconstruction, and are easily wrapped by tissue fibers when transplanted into the body

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