Active microbial agent for deodorizing livestock and poultry feces and preparation method thereof

A technology of odor active bacterial agent and poultry manure, which is applied in the field of waste deodorizing active bacterial agent and its preparation, can solve the problems of cumbersome steps and achieve the effects of simple process, improved processing speed and utilization rate, and stable structure

Inactive Publication Date: 2016-06-01
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cost of biocomposting is low, special equipment is required, and sewage and fecal solids are treated separately, and the operation steps are cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Composition of deodorant active bacteria

[0024] Bacillus subtilis B.subtilisCZ9, 2.0×10 8 cfu / ml;

[0025] Bacillus cereus B.cereusSZ2, 5.0×10 7 cfu / ml

[0026] Arthrobacter sp.SZ16, 1.0×10 8 cfu / ml

[0027] Streptomyces sp.SZ36, 1.0×10 8 cfu / ml.

[0028] Saccharomyces S.roseusCZ13, 5.0×10 7 cfu / ml.

[0029] Lactococcus S.lactisCZ15, 3.0×10 8 cfu / ml.

[0030] The bacterial agent with the above composition is prepared by the following steps:

[0031] 1) Single-strain fermentation: Ferment six strains of Bacillus subtilis CZ9, Bacillus cereus SZ2, Arthrobacter sp. .

[0032] Bacillus subtilis B.subtilisCZ9 and Bacillus cereus B.cereusSZ2 were cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride); 1% inoculum was inoculated, 180rpm, cultured for 24h .

[0033] Streptomycessp.SZ36 uses SCP medium (1.0% sucrose, 2.0% corn flour, 0.3% peptone, 3.0% bean cake powder, 0.1% potassium dihydrogen phosphate, 0.5% ammonium sulfate,...

Embodiment 2

[0050] Composition of deodorant active bacteria

[0051] Bacillus subtilis B.subtilisCZ9, 3.0×10 8 cfu / ml;

[0052] Bacillus cereus B. cereusSZ2, 1.0×10 8 cfu / ml

[0053] Arthrobacter sp.SZ16, 1.5×10 8 cfu / ml

[0054] Streptomyces sp.SZ36, 2.0×10 8 cfu / ml.

[0055] Saccharomyces S.roseusCZ13, 1.5×10 8 cfu / ml.

[0056] Lactococcus S.lactisCZ15, 5.0×10 8 cfu / ml.

[0057] 1) Single-strain fermentation: Ferment six strains of Bacillus subtilis CZ9, Bacillus cereus SZ2, Arthrobacter sp. .

[0058] Bacillus subtilis B.subtilisCZ9 and Bacillus cereus B.cereusSZ2 were cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride); 1.5% inoculum size, 190rpm, cultured for 30h.

[0059] Streptomycessp.SZ36 uses SCP medium (1.0% sucrose, 2.0% corn flour, 0.3% peptone, 3.0% bean cake powder, 0.1% potassium dihydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate ) culture; inoculate with 1.5% inoculation amount, 190 ...

Embodiment 3

[0076] Composition of deodorant active bacteria

[0077] Bacillus subtilis B.subtilisCZ9, 5.0×10 8 cfu / ml;

[0078] Bacillus cereus B. cereusSZ2, 2.0×10 8 cfu / ml

[0079] Arthrobacter sp.SZ16, 2.0×10 8 cfu / ml

[0080] Streptomyces sp.SZ36, 3.0×10 8 cfu / ml.

[0081] Saccharomyces S.roseusCZ13, 3.0×10 8 cfu / ml.

[0082] Lactococcus S.lactisCZ15, 8.0×10 8 cfu / ml.

[0083] 1) Single-strain fermentation: Ferment six strains of Bacillus subtilis CZ9, Bacillus cereus SZ2, Arthrobacter sp. .

[0084] Bacillus subtilis B.subtilisCZ9 and Bacillus cereus B.cereusSZ2 were cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride); 2.0% inoculum was inoculated, 200rpm, cultured for 36h .

[0085] Streptomycessp.SZ36 uses SCP medium (1.0% sucrose, 2.0% corn flour, 0.3% peptone, 3.0% bean cake powder, 0.1% potassium dihydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate ) culture; inoculate with 2.0% inoculation ...

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Abstract

The invention discloses an active microbial agent for deodorizing livestock and poultry feces. The active microbial agent comprises six types of strains, namely bacillus subtilis, bacillus cereus, arthrobacterium, streptomycete, saccharomycetes and lactic streptococci. The invention further discloses a preparation method of the active microbial agent for deodorizing livestock and poultry feces. The active microbial agent for deodorizing livestock and poultry feces and the preparation method thereof utilize synergism of the six types of feces degrading bacteria or deodorization bacteria and have the advantages of being novel in strain combination, stable in structure, great in enzyme activity, large in bacteria number and the like, meanwhile, the problems that in traditional compost treatment of livestock and poultry feces, feces-urine separation is required, feces degradation is low in speed, and the deodorizing effect is not obvious are solved, and the livestock and poultry feces treatment speed and utilization rate are raised.

Description

technical field [0001] The invention relates to the field of ecological breeding, in particular to a deodorizing active bacterial agent for poultry manure and other wastes and a preparation method thereof. Background technique [0002] With the development of my country's livestock and poultry breeding industry and the implementation of the "Vegetable Basket Project", the large-scale and intensive livestock and poultry breeding industry has risen rapidly, and the production of livestock and poultry manure has increased year by year. The freshly discharged poultry manure contains harmful gases such as NH3, H2S and indole. If it is not removed in time or not processed in time after removal, the odor will multiply and produce methyl sulfide, dimethyl disulfide, methyl sulfide, etc. Odor gases such as ether, dimethylamine and lower fatty acids. Calculations show that a pig farm with an annual slaughter of 5,000 pigs produces more than 0.8Kg of ammonia per day. The malodorous g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C05F3/00C05F17/00C12R1/125C12R1/085C12R1/06C12R1/465C12R1/865C12R1/46
CPCC05F3/00C05F17/00C12N1/18C12N1/20Y02A40/20Y02W30/40
Inventor 黄胜威高战元吴丽芳潘高志蔡冬清吴正岩李明浩赵薇薇
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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