Multi-stage fermentation production method for highly yielding spore clostridium butyricum

A technology of Clostridium butyricum and a production method, which is applied in the field of fermentation technology of Clostridium butyricum, can solve the problems of insufficient anaerobic conditions, affecting product quality, lack of feeding process, etc., and achieves the promotion of bacterial species reproduction and significant economic benefits. , the effect of reducing mortality

Inactive Publication Date: 2018-09-14
ANHUI TECH BANK BIO TECH
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing clostridium butyricum anaerobic fermentation process has the following defects: (1) anaerobic conditions are not sufficient, and a small amount of oxygen remains easily; (2) the fermentation process lacks feeding technology; (3) the number of fermented bacteria obtained is 10- 15×10

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-stage fermentation production method for highly yielding spore clostridium butyricum

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1:

[0023] A multi-stage fermentation production method of high-yielding clostridium butyricum includes the following steps:

[0024] (1) Prepare 300ml seed medium, which contains 3g yeast extract, 10g beef extract, 10g tryptone, 5g glucose, 1g soluble starch, 5g sodium chloride, and 3g sodium acetate trihydrate per 1000ml of distilled water. 0.5g of cysteine ​​hydrochloride, 0.2ml of 0.5% methylene blue, use 10% sodium hydroxide solution to adjust the pH of the seed medium to 7.1, cover the surface with 2cm liquid paraffin, sterilize at 121°C for 20 minutes, Cool to room temperature, inoculate 1% (relative to the seed culture medium) of Clostridium butyricum bacteria solution, seal it and place it in an incubator at 37°C for 24 hours, and keep it in a water bath at 80°C for 10 minutes to prepare a first-level seed solution culture;

[0025] (2) After the 50L seed tank is empty, add 35L seed culture medium, use 10% sodium hydroxide solution to adjust the pH to 7.1, c...

Example Embodiment

[0029] Example 2:

[0030] A multi-stage fermentation production method of high-yielding clostridium butyricum includes the following steps:

[0031] (1) Prepare 300ml seed medium, which contains 3g yeast extract, 10g beef extract, 10g tryptone, 5g glucose, 1g soluble starch, 5g sodium chloride, and 3g sodium acetate trihydrate per 1000ml of distilled water. 0.5 g of cysteine ​​hydrochloride, 0.2 mL of 0.5% methylene blue, use 10% sodium hydroxide solution to adjust the pH of the seed medium to 7.1, cover the surface with 3 cm of liquid paraffin, sterilize at 121°C for 20 minutes, Cool to room temperature, inoculate 1% (relative to the seed culture medium) of Clostridium butyricum bacteria solution, seal it and place it in an incubator at 37°C for 24 hours, and keep it in a water bath at 80°C for 10 minutes to prepare a first-level seed solution culture;

[0032] (2) After the 50L seed tank is empty, add 35L seed culture medium, use 10% sodium hydroxide solution to adjust the pH to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of a clostridium butyricum fermentation process, in particular to a multi-stage fermentation production method for highly yielding spore clostridium butyricum. The method comprises the steps of seed liquid preparation, primary oxygen fermentation culture, secondary fermentation culture and adsorption drying, wherein glucose is supplemented in the secondary fermentation process; the temperature and the pH value are controlled in the whole process; the low-speed stirring fermentation culture is performed in the whole process; the dissolved oxygen riskis eliminated. The spore formation rate is a hundred percent; the microbial content of the clostridium butyricum of the bacterium liquid is high; the spore loss rate of the clostridium butyricum is low; the multi-stage fermentation is more favorable for the high-temperature resistance of the spores; the microbial content in the bacterium liquid is 2.5 to 3 billion/ml; the heat loss of the bacterium powder in the adsorption drying process is improved; the live spore number of clostridium butyricum in the bacterium powder is improved by 1 to 1.5 times; the microbial content in the bacterium powder reaches 0.4 to 0.5 billion/g; the live spore number and the butyrate content in the bacterium powder are improved; the product quality and the competitive power are improved; the obvious economicbenefits are realized.

Description

technical field [0001] The invention relates to the technical field of Clostridium butyricum fermentation technology, in particular to a multistage fermentation production method for high-yielding spore-forming Clostridium butyricum. Background technique [0002] Clostridium butyricum, Clostridium butyricum, also known as butyric acid bacteria, is a species in the genus Clostridium, which exists in cheese, natural yogurt, human and animal intestines and feces. Clostridium butyricum is a straight or curved Gram-positive anaerobic endophytic bacillus. It is a probiotic strain that has been recognized by research. It is widely used in intestinal medicine and health food in clinical medicine and animal husbandry. , feed additives and microbial fertilizers, etc., is an ideal microecological preparation with broad development and application prospects. Clostridium butyricum is resistant to gastric acid and bile salts when it passes through the intestinal tract of animals, which e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N3/00C12R1/145
CPCC12N1/20C12N3/00
Inventor 张邦辉葛桥胡传炯薛兵
Owner ANHUI TECH BANK BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap