Pseudomonas aeruginosa fermentation medium, fermentation culture method thereof and vaccine preparation method

A fermentation medium and fermentation culture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of not fully utilizing the nutrient content of the medium, cumbersome process, and easy pollution

Inactive Publication Date: 2016-03-23
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can not make full use of the nutrients in the culture medium, the number of bacteria is low, the output is low, the process is cumbersome, the production cycle is long, the production cost is high, the production volume is low, and it is easy to pollute. It is suitable for small batch production in the early stage of vaccine development, but not suitable for large-scale production. chemical production
[0005] The patent application number CN201210095852.4 discloses a mink Pseudomonas aeruginosa propolis inactivated vaccine and its preparation process. It mentions the production of Pseudomonas aeruginosa with a fermenter, and the bacteria are added to the vaccine containing 2% fetal bovi

Method used

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  • Pseudomonas aeruginosa fermentation medium, fermentation culture method thereof and vaccine preparation method
  • Pseudomonas aeruginosa fermentation medium, fermentation culture method thereof and vaccine preparation method
  • Pseudomonas aeruginosa fermentation medium, fermentation culture method thereof and vaccine preparation method

Examples

Experimental program
Comparison scheme
Effect test

Example

[0178] Examples 1 to 3 Fermentation medium and fermentation culture method

[0179] The composition of the fermentation medium in this example is shown in the following table:

[0180] Table 1 Fermentation medium composition

[0181]

[0182]

[0183] The fermentation production process is as follows:

[0184] Primary seed preparation: Take one lyophilized strain of strain DL15 and strain JL08, unsealed, dilute with sterilized physiological saline, inoculate PYG agar medium, culture at 37°C for 24 hours, select more than 5 typical colonies and mix with a small amount of PYG In the liquid medium, inoculate a few more PYG agar slant medium, cultivated at 37°C for 24 hours, as the first-level seeds. Stored at 2~8℃, the use period does not exceed 15 days, and the passage does not exceed 5 generations.

[0185] Preparation of secondary seeds: inoculate several 5mLPYG liquid medium from the primary seeds, shake for 12 hours at 37°C, then inoculate a larger amount of PYG liquid medium at a r...

Example

[0192] Examples 4-6 Fermentation medium and fermentation culture method

[0193] The composition of the fermentation medium in this example is shown in the following table:

[0194] Table 3 Fermentation medium composition

[0195] Component

[0196] The fermentation production process is as follows:

[0197] Primary seed preparation: Take one lyophilized strain of strain DL15 and strain JL08, unsealed, dilute with sterilized physiological saline, inoculate PYG agar medium, culture at 37°C for 24 hours, select more than 5 typical colonies and mix with a small amount of PYG In the liquid medium, inoculate a few more PYG agar slant medium, cultivated at 37°C for 24 hours, as the first-level seeds. Stored at 2~8℃, the use period does not exceed 15 days, and the passage does not exceed 5 generations.

[0198] Preparation of secondary seeds: inoculate several 5mLPYG liquid medium from the primary seeds, shake for 12 hours at 37°C, then inoculate a larger amount of PYG liquid medium at a ...

Example

[0205] Examples 7-9 Fermentation medium and fermentation culture method

[0206] The composition of the fermentation medium in this example is shown in the following table:

[0207] Table 5 Fermentation medium composition

[0208] Component

[0209] The fermentation production process is as follows:

[0210] Primary seed preparation: Take one lyophilized strain of strain DL15 and strain JL08, unsealed, dilute with sterilized physiological saline, inoculate PYG agar medium, culture at 37°C for 24 hours, select more than 5 typical colonies and mix with a small amount of PYG In the liquid medium, inoculate a few more PYG agar slant medium, cultivated at 37°C for 24 hours, as the first-level seeds. Stored at 2~8℃, the use period does not exceed 15 days, and the passage does not exceed 5 generations.

[0211] Preparation of secondary seeds: inoculate several 5mLPYG liquid medium from the primary seeds, shake for 12 hours at 37°C, then inoculate a larger amount of PYG liquid medium at a ...

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Abstract

The invention relates to the technical field of fermentation culture, in particular to a pseudomonas aeruginosa fermentation medium, a fermentation culture method thereof and a vaccine preparation method. The fermentation medium comprises peptone, glucose, yeast powder, sodium chloride, Na2HPO4, KH2PO4, MgSO4, sodium citrate, urea and decavitamin B. The fermentation medium is suitable for high-density fermentation and growth of pseudomona aeruginosa, and the viable count of pseudomonas aeruginosa can be greatly increased; compared with an original traditional medium, the improved medium prolongs the logarithmic phase from 4-8 h to 4-10 h remarkably and increases the microbial content from 14-15.9 billion to 24-25 billion, the pH (potential of hydrogen) value is kept at 7.0, and the yield of pseudomonas aeruginosa is greatly increased.

Description

technical field [0001] The invention relates to the technical field of fermentation culture, in particular to a Pseudomonas aeruginosa fermentation medium, a fermentation culture method thereof, and a vaccine preparation method. Background technique [0002] Mink hemorrhagic pneumonia is an acute infectious disease of mink caused by Pseudomonas aeruginosa. It mostly occurs in warm and humid autumn. Pulmonary edema is the main lesion characteristic, the incidence rate is 20%-50%, and the mortality rate is 100%. Sick mink has acute onset and rapid death, often showing endemic outbreaks. In recent years, due to the obvious increase in the scale and intensification of mink farming, the incidence of mink hemorrhagic pneumonia has increased significantly, and the degree of harm of the disease has increased year by year. It has become one of the main infectious diseases that endanger mink farming in my country. [0003] In view of the onset characteristics of the disease and the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/104A61P11/00A61P31/04C12R1/385
CPCA61K39/104C12N1/20
Inventor 白雪闫喜军柴秀丽吴威王长凤赵建军张海玲张蕾胡博赵传芳罗国良
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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