Pseudomonas aeruginosa fermentation medium, fermentation culture method thereof and vaccine preparation method

Abstract

The invention relates to the technical field of fermentation culture, in particular to a pseudomonas aeruginosa fermentation medium, a fermentation culture method thereof and a vaccine preparation method. The fermentation medium comprises peptone, glucose, yeast powder, sodium chloride, Na2HPO4, KH2PO4, MgSO4, sodium citrate, urea and decavitamin B. The fermentation medium is suitable for high-density fermentation and growth of pseudomona aeruginosa, and the viable count of pseudomonas aeruginosa can be greatly increased; compared with an original traditional medium, the improved medium prolongs the logarithmic phase from 4-8 h to 4-10 h remarkably and increases the microbial content from 14-15.9 billion to 24-25 billion, the pH (potential of hydrogen) value is kept at 7.0, and the yield of pseudomonas aeruginosa is greatly increased.

Description

technical field [0001] The invention relates to the technical field of fermentation culture, in particular to a Pseudomonas aeruginosa fermentation medium, a fermentation culture method thereof, and a vaccine preparation method. Background technique [0002] Mink hemorrhagic pneumonia is an acute infectious disease of mink caused by Pseudomonas aeruginosa. It mostly occurs in warm and humid autumn. Pulmonary edema is the main lesion characteristic, the incidence rate is 20%-50%, and the mortality rate is 100%. Sick mink has acute onset and rapid death, often showing endemic outbreaks. In recent years, due to the obvious increase in the scale and intensification of mink farming, the incidence of mink hemorrhagic pneumonia has increased significantly, and the degree of harm of the disease has increased year by year. It has become one of the main infectious diseases that endanger mink farming in my country. [0003] In view of the onset characteristics of the disease and the ...

AI Technical Summary

Problems solved by technology

This method can not make full use of the nutrients in the culture medium, the number of bacteria is low, the output is low, the process is cumbersome, the production cycle is long, the production cost is high, the production volume is low, and it is easy to pollute. It is suitable for small batch production in the early stage of vaccine development, but not suitable for large-scale production. chemical production

[0005] The patent application number CN201210095852.4 discloses a mink Pseudomonas aeruginosa propolis inactivated vaccine and its preparation process. It mentions the production of Pseudomonas aeruginosa with a fermenter, and the bacteria are added to the vaccine containing 2% fetal bovine serum by 2% volume. In the fermentation medium, cultivate at 37°C with aeration and stirring for 12-16 hours, and use formaldehyde with a final concentration of 0.5% to inactivate for 24 hours. This invention does not mention the use of medium components, and does not mention the production process such as ventilation, pH value, air pressure , the inoculation ratio and storage time of the seed solution, and the inactivation concentration of formaldehyde used is too high

The applicant uses the conventional fermentation medium and fermentation parameters to ferment according to the patent method, and the number of bacteria produced is small, which cannot meet the requirements of industrialization

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