Compound bacterial agent applied to straw mulching and preparation method thereof

A compound bacterial agent and straw technology, which is applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve the problem of inability to achieve fast and efficient straw decomposition effects, single microbial metabolism type and function, and difficulty in large-scale promotion To achieve the effect of promoting sustainable development, reducing environmental pollution, and improving the efficiency of straw decomposition

Inactive Publication Date: 2016-01-13
上海原本生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the decomposing bacterial agents for crop straw returning to the field have problems such as single microbial metabolism type and function, poor pertinence, and improper compounding ratio, which cannot achieve fast and efficient straw decomposition effects, and are difficult to promote and apply on a large scale
At the same time, among the existing fermentation agents, there are many thermophilic cellulolytic bacteria, and the research on microbial strains or flora that decompose cellulose, lignin, and wax by normal temperature (low temperature) fermentation is far behind the production needs.
Returning straw directly to the field can improve soil fertility, but it also provides a suitable growth environment for the growth and development of pests and diseases, increasing the chance of the next crop being harmed

Method used

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  • Compound bacterial agent applied to straw mulching and preparation method thereof
  • Compound bacterial agent applied to straw mulching and preparation method thereof
  • Compound bacterial agent applied to straw mulching and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Composition of complex microbial agent

[0028]

[0029]

[0030] The bacterial agent with the above composition is prepared by the following steps:

[0031] 1) Carrier preparation: uniformly mix attapulgite (200 mesh), peat (200 mesh) and vermiculite (200 mesh) according to the mass ratio of 2.5:0.5:1 to obtain a carrier, and set aside;

[0032] 2) Preparation of protective agent: uniformly mix trehalose (200 mesh) and sodium glutamate (200 mesh) according to a mass ratio of 1:1.5 to obtain a protective agent, and set aside;

[0033] 3) Fermentation of single strains: four strains of Bacillus sp.CZ5, Arthrobacter sp.WH16, Streptomyces sp.WH36 and Micromonospora sp.WH13 were respectively fermented to obtain fermentation broth.

[0034] Bacillus subtilis was cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride); 1% inoculum was inoculated in LBG liquid medium, 180 rpm, and cultured for 24 hours.

[0035] Streptomyces chestnutus...

Embodiment 2

[0058] Composition of complex microbial agent

[0059]

[0060]

[0061] The bacterial agent with the above composition is prepared by the following steps

[0062] 1) Carrier preparation: uniformly mix attapulgite (300 mesh), peat (300 mesh) and vermiculite (300 mesh) according to the mass ratio of 2.5:0.8:1 to obtain a carrier, and set aside;

[0063] 2) Preparation of protective agent: uniformly mix trehalose (300 mesh) and sodium glutamate (300 mesh) according to a mass ratio of 1:1.8 to obtain a protective agent, and set aside;

[0064] 3) Single-strain fermentation: four strains of Bacillus sp.CZ5, Arthrobacter sp.WH16, Streptomycessp.WH36 and Micromonospora sp.WH13 were fermented respectively to obtain fermentation broth.

[0065] Bacillus subtilis was cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride); 1.5% inoculum was inoculated in LBG liquid medium, 190 rpm, and cultured for 30 h.

[0066] Streptomyces chestnutus uses SC...

Embodiment 3

[0089] Composition of complex microbial agent

[0090]

[0091] The bacterial agent with the above composition is prepared by the following steps

[0092] 1) Carrier preparation: uniformly mix attapulgite (400 mesh), peat (400 mesh) and vermiculite (400 mesh) according to the mass ratio of 2.5:1:1 to obtain a carrier, and set aside;

[0093] 2) Preparation of protective agent: uniformly mix trehalose (400 mesh) and sodium glutamate (400 mesh) according to a mass ratio of 1:2 to obtain a protective agent, and set aside;

[0094] 3) Single-strain fermentation: four strains of Bacillus sp.CZ5, Arthrobacter sp.WH16, Streptomycessp.WH36 and Micromonospora sp.WH13 were fermented respectively to obtain fermentation broth.

[0095] Bacillus subtilis was cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride); 2% inoculum was inoculated in LBG liquid medium, 200 rpm, and cultured for 36 hours.

[0096]Streptomyces chestnutus uses SCP medium (1.0% ...

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Abstract

The invention discloses a compound bacterial agent applied to straw mulching. The compound bacterial agent comprises 1.0*10<9>cfu/g-8.0*10<9>cfu/g of Bacillus sp.CZ5, 1.0*10<8>cfu/g-6.0*10<9>cfu/g of Arthrobacter sp.WH16, 1.0*10<9>cfu/g-2.0*10<10>cfu/g of Streptomyces sp.WH36 and 1.0*10<7>cfu/g-5.0*10<8>cfu/g of Micromonospora sp.WH13. The invention further provides a preparation method of the compound bacterial agent applied to straw mulching. Compared with the prior art, the compound bacterial agent applied to straw mulching has the advantage that purposes of quick decomposition of straw, suppression of soil pathogens and increase of crop yield are achieved under synergistic effect of various straw degradation bacteria/biocontrol bacteria.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a composite bacterial agent for straw returning to the field and a preparation method thereof. Background technique [0002] my country is rich in crop straw resources, with a huge quantity and various types. The annual output of crop straw is 700-800 million tons. Since the straw is not easy to decompose in a short period of time, the actual utilization efficiency is low. In addition to a small amount of straw being used for gaskets, feeding livestock, composting manure, and rural fuelwood, a large part of straw is poured into the environment in the form of stacking and burning, which not only causes serious pollution to the environment, but also causes an extreme waste of resources. Big waste. Therefore, the efficient resource utilization of straw can not only reduce pollution and protect the environment, but also turn waste into treasure and promote sustainable econo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/06C12R1/465C12R1/29
Inventor 吴丽芳黄胜威蔡冬清李明浩吴正岩赵薇薇熊世权
Owner 上海原本生物科技有限公司
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