A kind of marine Micromonospora strain producing rakicidin A by fermentation and its application

A kind of strain and marine technology, applied in the field of marine Micromonospora strains fermented to produce RakicidinA, can solve the problem of low fermentation yield, achieve the effect of increasing potency and meeting the needs of industrialization
CN108130284BActive Publication Date: 2019-03-05FUJIAN INST OF MICROBIOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUJIAN INST OF MICROBIOLOGY
Publication Date
2019-03-05

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Abstract

The invention belongs to the technical field of microbial fermentation, and in particular relates to a marine Micromonospora strain producing Rakicidin A by fermentation and application thereof. The present invention obtains a high-yield Rakicidin A mutant strain FIM-R150103 by performing mutation breeding on the existing Rakicidins compound-producing Micromonospora marine strain FIM-R150103, which can effectively increase the content of Rakicidin A in the fermentation broth. Potency, in the fermentation experiment of 20‑1000L fermenter, the titer of Rakicidin A produced by Micromonospora marina FIM‑R150103 was as high as 300 mg / L, which greatly facilitated the extraction and purification of Rakicidin A and could meet the needs of industrialization .
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Description

technical field

[0001] The invention belongs to the technical field of microbial fermentation, in particular to a marine Micromonospora strain producing Rakicidin A by fermentation and application thereof. Background technique

[0002] Rakicidins are the fermentation products of Micromonospora marine. The currently isolated Rakicidins mainly contain Rakicidin A, Rakicidin A1, Rakicidin B1 and Rakicidin B. Their chemical structures and components are as follows:

[0003]

[0004] Rakicidins have attracted attention because their 15-membered cyclic lipopeptide structure contains a rare 4-amino-2,4-pentadienoic acid octanamide and has anti-tumor cell activity. Yamazaki (2007) found that Rakicidin A has excellent hypoxic selective anti-tumor cell activity, and the anti-colon cancer HCT-8 cell activity under hypoxic conditions is 17.5 times that under normal oxygen conditions; Takeuchi (2011) also reported for the first time Rakicidin A can induce the apoptosis of myeloid chr...

Claims

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