Composite culture medium capable of simultaneously enriching three food-borne pathogenic bacteria and preparation method

Abstract

The invention discloses a composite enrichment culture medium capable of simultaneously enriching three food-borne pathogenic bacteria, namely Listeria monocytogenes, Escherichia coli O157:H7 and salmonella, and a preparation method. The formula of the culture medium comprises the following components of, in parts by weight, 15.0-19.0 parts of tryptone, 2.7-3.3 parts of peptone, 5.5-6.5 parts of yeast extract, 4.5-5.5 parts of sodium chloride, 2.25-2.75 parts of dipotassium phosphate, 8.6-10.6 parts of disodium hydrogen phosphate, 1.22-1.48 parts of potassium dihydrogen phosphate, 24.0-30.0 parts of peptone, 14.0-18.0 parts of beef powder, 10.0-14.0 parts of soy peptone, 2.0-5.0 parts of glucose, 0.10-0.30 part of actinomycete ketone, 2.5-3.5 parts of mannitol, 4.5-6.5 parts of sodium pyruvate, 0.00125-0.00300 part of acridine yellow, 0.0007813-0.0032500 part of fosfomycin sodium and 1000 parts of distilled water, and the pH value of theculture medium is 6.8-7.4. The single enrichmenteffect of the composite enrichment culture medium is superior to that of respective selective enrichment liquid, growth of non-target bacteria can be well inhibited, under the condition that non-target bacteria exist, the culture medium can further achieve constant-speed and rapid proliferation of the three target bacteria.

Description

technical field [0001] The invention belongs to the technical field of pre-enrichment medium for pathogenic bacteria, and in particular relates to a composite medium capable of simultaneously enriching three kinds of food-borne pathogenic bacteria, that is, Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella. Preparation. Background technique [0002] In recent years, foodborne diseases caused by eating food contaminated by foodborne pathogens such as Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella have often occurred. The control and detection of foodborne pathogenic bacteria have been highly valued all over the world. [0003] Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella are three common foodborne pathogens that must be detected in my country's national food safety standards. Usually, the number of foodborne pathogenic bacteria that may exist in food is often relatively small. In order to ensure the accuracy of the test resu...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems in the prior art that different enrichment media are often used for cultivating different pathogenic bacteria, which increases the workload of detection, the present invention provides a method that can simultaneously cultivate Listeria monocytogenes and Escherichia coli The compound enrichment medium and preparation method of O157:H7 and Salmonella are suitable for high-throughput detection technologies such as multiplex PCR and multi-channel BLI biosensors, and help to realize the simultaneous and rapid detection of three pathogenic bacteria

Images