Formula and preparation method of culture medium used for Niannianle seed-streptomyces microflavus from cultivation to fermentation

A technology of Streptomyces flavinus and culture medium, applied in the direction of microorganism-based methods, bacteria, biochemical equipment and methods, etc., can solve the problem of poor fermentation yield and effect, low fermentation yield, and easy contamination of miscellaneous bacteria And other issues

Inactive Publication Date: 2013-09-18
唐睿
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Streptomyces flavinus is often affected by factors such as seed quality, medium composition, bacterial contamination, process conditions, and metabolic regulation of the fermentation process during the fermentation process, and the fermentation yield and effect are not good.
[0004] The main purpose of the present invention is to develop a set of strains from seed cultivation to deficiencies such as slow propagation speed of strains, low fermentation yield, easy contamination of miscellaneous bacteria and easy degradation of strains that existed in the cultivation and fermentation process of Streptomyces flavinus in the past. Fermentation production, the culture medium with the advantages of fast propagation speed, high yield, high ratio of effective viable bacteria, less contamination by bacteria, and cheap fermentation materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1. The effects of adding special substances: trehalose, potassium dichromate and cycloheximide respectively on the solid medium.

[0035] The specific test steps are:

[0036] (1) Prepare culture medium and sterilize.

[0037] CK (Gaoshi No. 1): Soluble starch 20.0g, KNO 3 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl0.5g, FeSO 4 ·7H 2 O0.01g, agar 20g, distilled water 1000mL, pH7.2-7.4.

[0038] 1#: Cycloheximide 40, 60, 80, 100, 120 mg, respectively added on the basis of CK.

[0039] 2#: Potassium dichromate 30, 50, 70, 90 mg, respectively added on the basis of CK.

[0040] 3#: Trehalose 3.0, 3.5, 4.0, 4.5, 5.0g were added on the basis of CK respectively.

[0041] (2) Use a sterilized puncher with the same aperture and rejuvenated strains with the same age to punch out the bacteria cakes, and inoculate them respectively, with 3 repetitions per culture medium, and 1 bacteria cake per repetition, after culturing for 3-5 days , it was observed that...

Embodiment 2

[0043] Example 2. The effects of adding special substances: trehalose, potassium dichromate and cycloheximide respectively on the liquid medium.

[0044] (2) Prepare culture medium and sterilize.

[0045] CK (Gaoshi No. 1): Soluble starch 20.0g, KNO 3 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl0.5g, FeSO 4 ·7H 2 O0.01g, agar 20g, distilled water 1000mL, pH7.2-7.4.

[0046] 1#: Cycloheximide 40, 60, 80, 100, 120 mg, respectively added on the basis of CK.

[0047] 2#: Potassium dichromate 30, 50, 70, 90 mg, respectively added on the basis of CK.

[0048] 3#: Trehalose 3.0, 3.5, 4.0, 4.5, 5.0g were added on the basis of CK respectively.

[0049] (2) Use a hole puncher with the same aperture and strains with the same bacterial age to punch out bacterial cakes and inoculate them respectively. Each culture medium is repeated 3 times, and each repetition is inoculated with 250ml of 50ml medium and 1 bacterial cake inoculum. , 180r / min on a shaking table, cultured at 30°C...

Embodiment 3

[0051] Embodiment 3. Screening of a fermentation production medium of Streptomyces flavinus.

[0052] (1) Mixing of raw and auxiliary materials:

[0053] CK: 20g bean cake powder, 20g glucose, 3g NaCl, CaCO 3 1g, 1000ml water, pH7.2-7.5.

[0054] Medium 1: 10g bean cake powder, 10g corn starch, 10g cassava flour, 1g fish meal, 3g NaCl, CaCO 3 1g, trehalose 3g, cycloheximide 80mg, potassium dichromate 60mg, water 1000ml, pH7.2-7.5.

[0055] Medium No. 2: bean cake powder 10g, corn flour 10g, glucose 10g, sucrose maltose 4g, trehalose 3g, cycloheximide 80mg, potassium dichromate mg, 60NaCl3g, CaCO 3 1g, 1000ml water, pH7.2-7.5.

[0056] Medium 3: 10g bean cake powder, 10g peanut shell, 10g millet flour, 1g fish meal, 3g NaCl, CaCO 3 1g, trehalose 3g, cycloheximide 80mg, potassium dichromate 60mg, water 1000ml, pH7.2-7.5.

[0057] (2) Autoclaving: Sterilize by passing superheated steam, 1.5 kg / cm2, 121°C, 30min.

[0058] (3) Inoculation: when the temperature of the raw and...

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Abstract

The invention relates to a formula and preparation method of a culture medium used for a strain of actinomycetes Niannianle: ACCC40027 streptomyces microflavus, from cultivation and storage to fermentation production; moreover, the culture medium can ensure high yield and hardly degraded. And the following special materials are added: mycose, cycloheximide and potassium dichromate.

Description

technical field [0001] The invention relates to a strain of actinomycete "Niannianle": ACCC40027 Streptomyces microflavus, a high-yield and non-degradable medium formula and a preparation method thereof, and special substances added thereto Yes: trehalose, cycloheximide, potassium dichromate. technical background [0002] In the biological control of plant diseases, actinomycetes are the first biocontrol microorganisms used in production. At present, nearly 70% of the approximately 8,000 biologically active substances found in microorganisms are produced by actinomycetes, which have important economic importance. value. The antibiotics produced by them can effectively prevent and treat infectious diseases of humans and animals, especially bacterial diseases and certain diseases of crops, and play a huge role. And the new pesticides prepared by using the secondary metabolites of actinomycetes-"antibiotics" have become the main body of pollution-free pesticides and future pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/465
Inventor 任承才唐睿韩铁冰莫秋菊杨照东吴景霄
Owner 唐睿
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