Nitrification inhibitor for promoting root hair growth, biostimulant and preparation method and application thereof

Abstract

The invention provides a nitrification inhibitor for promoting root hair growth, a biostimulant and a preparation method and application of the biostimulant, the nitrification inhibitor comprises 3, 4-dimethylpyrazole and streptomyces fermentation liquor with the weight ratio of 1: (2.5-4.0), and the streptomyces fermentation liquor contains organic acid. The nitrification inhibitor provided by the embodiment of the invention can effectively promote the growth of root hair, further, the biostimulant prepared from the nitrification inhibitor can effectively induce up-regulation expression of auxin related genes ZmARF10, ZmYUC2, ZmYUC3, ZmYUC5, ZmPIN1 and ZmPIN9, the growth of crop root hair is promoted, the root activity of plants is greatly improved, the high-quality growth and yield of crops are promoted, and meanwhile, the biostimulant has good application prospects. The raw materials of the biostimulant are easy to obtain, the preparation method is simple, and industrialization is easy to realize.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth, and in particular relates to a nitrification inhibitor and a biostimulant for promoting root hair growth, and a preparation method and application thereof. Background Art

[0002] The root system is the main part for crops to absorb nutrients, and root hairs play an important role in root nutrient absorption. Root hairs are tubular extensions of root epidermal cells, accounting for 77% of the root surface area. They are important sites for water and nutrient absorption, and are very important for obtaining nutrients, root anchoring, plant growth and development, and stress response. Root hair cells originate from root epidermal cells, but not all epidermal cells can develop into root hairs. Based on this, a substance is needed that can promote the growth of crop root hairs, promote the vitality of plant roots, and thereby enhance the ability of crop roots to absorb nutrients.

[0003] Biostimulants can improve nutrient utilization, reduce nutrient waste, and reduce the loss of nutrients in the environment. For example, biostimulants can promote root growth, promote the absorption of nutrients and water, and increase more soil organic carbon with fewer inputs. Biostimulants can also dissolve nutrients in the soil, such as phosphorus and nitrogen, increase the mobility of nutrients in plants, enable plants to better utilize nutrients, and reduce nutrient waste.

[0004] Biostimulants have a very good promoting effect on root growth, but most of the current biostimulants are generally broad-spectrum biostimulants. Broad-spectrum biostimulants do not have particularly outstanding effects on root growth, and there is a lack of biostimulants that specifically promote the growth of root hairs.

[0005] Therefore, there is an urgent need for a biostimulant that can specifically promote the growth of crop root hairs, enhance the vitality of plant roots, and promote high-quality growth of plants. Summary of the invention

[0006] The present invention aims to solve one of the technical problems in the related art to at least a certain extent. Therefore, the object of the present invention is to provide a nitrification inhibitor, a biostimulant and a preparation method and application thereof for promoting the growth of crop roots. The nitrification inhibitor provided by the present invention can effectively promote the growth of root hairs. Furthermore, the biostimulant prepared therefrom can effectively induce the up-regulation of auxin-related genes ZmARF10, ZmYUC2, ZmYUC3, ZmYUC5, ZmPIN1 and ZmPIN9, promote the growth of crop root hairs, greatly improve the vitality of plant roots, and promote the high-quality growth and yield of crops. At the same time, the raw materials of the biostimulant are easy to obtain, the preparation method is simple, and it is easy to realize industrialization.

[0007] Therefore, in the first aspect of the present invention, the present invention provides a nitrification inhibitor. According to an embodiment of the present invention, the nitrification inhibitor comprises 3,4-dimethylpyrazole and Streptomyces fermentation broth in a weight ratio of 1:(2.5-4.0), and the Streptomyces fermentation broth contains an organic acid. The organic acid present in the Streptomyces fermentation broth reacts with 2,4-dimethylpyrazole. The nitrification inhibitor according to the embodiment of the present invention can effectively promote the growth of plant root hairs, greatly improve the vitality of plant root systems, and promote the high-quality growth and yield of crops. The raw materials are easily available, the preparation method is simple, and it is easy to realize industrialization.

[0008] According to an embodiment of the present invention, the above-mentioned nitrification inhibitor may also include at least one of the following additional technical features:

[0009] According to an embodiment of the present invention, the Streptomyces fermentation broth is obtained from a Streptomyces fermentation medium, and the Streptomyces was deposited in the China Center for Type Culture Collection on September 28, 2022, with a preservation number of CGMCC No.25834.

[0010] According to an embodiment of the present invention, the culture medium includes 0.5% to 3% by mass of soluble starch, 0.1% to 0.3% by mass of potassium nitrate, 0.01% to 0.05% by mass of potassium dihydrogen phosphate, 0.01% to 0.05% by mass of magnesium sulfate and 0.01% to 0.05% by mass of sodium chloride.

[0011] According to an embodiment of the present invention, the fermentation broth is obtained by fermenting for 70 to 75 hours at 30° C. to 35° C., a shaking speed of 150 rpm to 250 rpm, and an aeration ratio of 1 to 1.5.

[0012] According to an embodiment of the present invention, the 3,4-dimethylpyrazole is preliminarily dissolved in an organic solvent at a mass ratio of 1:(2-12).

[0013] According to an embodiment of the present invention, the organic solvent comprises at least one of the following: methanol, ethanol, isopropanol and n-butanol. It will be appreciated by those skilled in the art that the selection condition of the organic solvent here is that it has high solubility for 3,4-dimethylpyrazole, low toxicity and low cost, and all organic solvents meeting the selection condition can be applied to the present application.

[0014] According to an embodiment of the present invention, the organic solvent is isopropanol.

[0015] In a second aspect of the present invention, the present invention provides a method for preparing a nitrification inhibitor. According to an embodiment of the present invention, the method comprises: mixing 3,4-dimethylpyrazole and Streptomyces fermentation broth at a weight ratio of 1:(2.5-4.0) to obtain the nitrification inhibitor.

[0016] According to the method prepared by the method of the embodiment of the present invention, the organic acid present in the Streptomyces fermentation broth reacts with 2,4-dimethylpyrazole, and the obtained nitrification inhibitor can effectively promote the growth of plant root hairs, greatly improve the vitality of the plant root system, and promote the high-quality growth and yield of crops. The raw materials are easy to obtain, the preparation method is simple, and it is easy to realize industrialization.

[0017] According to an embodiment of the present invention, the above method may further include at least one of the following:

[0018] According to an embodiment of the present invention, the fermentation liquid is obtained by fermenting Streptomyces in a culture medium, and the culture medium includes 0.5% to 3% soluble starch, 0.1% to 0.3% potassium nitrate, 0.01% to 0.05% potassium dihydrogen phosphate, 0.01% to 0.05% magnesium sulfate and 0.01% to 0.05% sodium chloride in a mass percentage. The inventors found that the nitrification inhibitor prepared by the fermentation liquid obtained by fermenting Streptomyces in the culture medium can more effectively promote the growth of plant root hairs, improve the vitality of plant roots, and promote the high-quality growth and yield of crops compared to the fermentation liquid obtained by other strains.

[0019] According to an embodiment of the present invention, the Streptomyces was deposited in the China Center for Type Culture Collection on September 28, 2022, with a deposit number of CGMCC No. 25834. Therefore, the nitrification inhibitor prepared by the fermentation broth obtained by the strain has a stronger function of promoting the growth of plant root hairs, improving the vitality of plant roots, and promoting the high-quality growth and yield of crops than the fermentation broth obtained by other strains.

[0020] According to an embodiment of the present invention, the fermentation is carried out for 70 to 75 hours at 30°C to 35°C, a shaking speed of 150 rpm to 250 rpm, and an aeration ratio of 1 to 1.5. The fermentation conditions are suitable for the growth of Streptomyces and can more efficiently obtain the above fermentation liquid.

[0021] According to an embodiment of the present invention, the 3,4-dimethylpyrazole is preliminarily dissolved in an organic solvent at a mass ratio of 1:(2-12).

[0022] According to an embodiment of the present invention, the organic solvent includes but is not limited to methanol, ethanol, isopropanol or n-butanol.

[0023] According to an embodiment of the present invention, the organic solvent is isopropanol.

[0024] According to an embodiment of the present invention, the temperature of the mixing reaction is 25°C to 55°C.

[0025] According to an embodiment of the present invention, the mixing reaction time is 3 to 12 hours.

[0026] In the third aspect of the present invention, the present invention provides a biostimulant. According to an embodiment of the present invention, the biostimulant includes: 23 to 68 parts by weight of the nitrification inhibitor described in the first aspect. The biostimulant according to the embodiment of the present invention can promote the synthesis of endogenous hormones such as crop root auxins, promote the growth of root hairs, greatly improve the vitality of crop roots, improve the ability of crop roots to absorb nutrients, and promote crop growth and yield.

[0027] According to an embodiment of the present invention, the above-mentioned biostimulant may also include at least one of the following additional technical features:

[0028] According to an embodiment of the present invention, the biostimulant further comprises: 5 to 15 parts by weight of amino acids, 1 to 10 parts by weight of peptides, 5 to 10 parts by weight of humic acid, and 20 to 40 parts by weight of elemental fertilizers.

[0029] According to an embodiment of the present invention, the amino acid includes at least one of tryptophan, glycine, arginine, glutamic acid and phenylalanine.

[0030] According to an embodiment of the present invention, the peptide comprises at least one of plant-derived small peptides, plant-derived polypeptides, animal-derived small peptides and animal-derived polypeptides. The peptide demand of the plant root system is met by adding peptides other than those fermented by microorganisms.

[0031] According to an embodiment of the present invention, the humic acid includes at least one of sodium humate, potassium humate, potassium fulvic acid, palmitic acid, fulvic acid, black humic acid, biochemical fulvic acid and brown-yellow fulvic acid.

[0032] According to an embodiment of the present invention, the elemental fertilizer includes at least one of urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, ammonium polyphosphate, urea-formaldehyde and urea phosphate.

[0033] According to an embodiment of the present invention, the biostimulant further comprises: 1 to 5 parts by weight of trace elements.

[0034] According to an embodiment of the present invention, the trace elements are not particularly limited, and the trace elements include but are not limited to at least one of zinc, manganese, boron and magnesium.

[0035] According to an embodiment of the present invention, the biostimulant further comprises: 1 to 2 parts by weight of an auxiliary agent.

[0036] According to an embodiment of the present invention, the auxiliary agent is not particularly limited, and the auxiliary agent includes but is not limited to: at least one of modified alkyl glycosides, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, glycerol polyoxyethylene ether polyoxypropylene ether fatty acid esters, alkyl succinate sulfonates, naphthalenesulfonic acid formaldehyde condensates, fatty alcohol polyoxyethylene ether sulfates, polymerized carboxylates and quaternary ammonium salts.

[0037] According to an embodiment of the present invention, the amino acids include: 2 to 4 parts by weight of glycine, 2 to 4 parts by weight of arginine, 2 to 4 parts by weight of tryptophan, and 0.5 to 1.5 parts by weight of glutamic acid. Thus, it can be ensured that the prepared biostimulant further targets and improves the root activity, promotes the growth of root hairs, and further greatly improves the root activity of plants. The inventors found that by adding corresponding parts by weight of glycine, glutamic acid and tryptophan, it is beneficial for the biostimulant to further improve the root activity, greatly improve the root activity of plants, and promote the high-quality growth and yield increase of plants; by adding corresponding parts by weight of phenylalanine, it is beneficial to improve the quality of plant color change; if the weight of the fermentation liquid is too low, it will lead to a decrease in the peptide content in the biostimulant, and the functional peptides that can be directly absorbed by the plant roots will be reduced, which is not conducive to improving the root activity. If the weight of the fermentation liquid is too high, it will lead to a corresponding decrease in the content of the remaining amino acids, which is not conducive to improving the root activity.

[0038] It should be noted that the order of introduction of the amino acids does not need to be specifically limited. After the amino acids are completely dissolved, trace elements and additives are introduced into the reactor in sequence, and the pH is controlled at 7-8. This makes it easier to obtain biostimulants.

[0039] According to an embodiment of the present invention, the peptide includes a plant-derived small peptide, thereby the plant-derived small peptide can more effectively promote the growth of root hair.

[0040] According to an embodiment of the present invention, the elemental fertilizer includes 10 to 20 parts by weight of monoammonium phosphate and 10 to 20 parts by weight of diammonium phosphate. Thus, the plant's demand for nitrogen (N) and phosphorus (P) can be balanced, nutrients can be saved, and thus the growth of the plant can be promoted and the quality and maturity of the product can be improved.

[0041] In a fourth aspect of the present invention, the present invention provides a method for preparing the biostimulant described in the third aspect. According to an embodiment of the present invention, the method comprises: (1) performing a first mixing treatment on the nitrification inhibitor described in the first aspect or the nitrification inhibitor obtained by the method described in the second aspect and elemental fertilizer. The biostimulant according to an embodiment of the present invention can promote the synthesis of endogenous hormones such as root auxin of crops, promote the growth of root hairs, greatly improve the vitality of crop roots, improve the ability of crop roots to absorb nutrients, and promote crop growth and yield increase.

[0042] According to an embodiment of the present invention, the method for preparing the biostimulant described in the third aspect may also include at least one of the following additional technical features:

[0043] According to an embodiment of the present invention, the method further comprises: (2) subjecting the first mixed treatment product to a second mixing treatment with amino acids, wherein the second mixing treatment is performed until the amino acids are completely dissolved; (3) subjecting the second mixed treatment product to a third mixing treatment with humic acid, wherein the third mixing treatment is performed until the humic acid is completely dissolved; and (4) subjecting the third mixed treatment product to a fourth mixing treatment with peptides to obtain the biostimulant.

[0044] According to an embodiment of the present invention, the first, second, third and fourth mixing treatments are performed at 20°C to 30°C.

[0045] According to an embodiment of the present invention, the method further comprises: subjecting the fourth mixing treatment product to a fifth mixing treatment with an auxiliary agent.

[0046] According to an embodiment of the present invention, the method further comprises: adjusting the pH of the fifth mixed treatment product to 7.5-8.5.

[0047] According to an embodiment of the present invention, the amino acid includes at least one of tryptophan, glycine, arginine, glutamic acid and phenylalanine. The selected amino acid is an important precursor substance for the biosynthesis of endogenous hormones such as auxin, and has a very good growth-promoting function.

[0048] According to an embodiment of the present invention, the peptide includes at least one of plant-derived small peptides, plant-derived polypeptides, animal-derived small peptides and animal-derived polypeptides. The selected peptide small molecule substances have very good stress resistance and growth-promoting functions. According to an embodiment of the present invention, the humic acid includes at least one of sodium humate, potassium humate, potassium fulvic acid, palm humic acid, fulvic acid, black humic acid, biochemical fulvic acid and brown-yellow humic acid.

[0049] According to an embodiment of the present invention, the elemental fertilizer includes at least one of urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, ammonium polyphosphate, urea formaldehyde and urea phosphate. Nitrogen (N), phosphorus (P) and potassium (K) are the three elements with the largest demand for crops, thereby promoting plant growth and improving product quality and maturity.

[0050] According to an embodiment of the present invention, the auxiliary agent includes: at least one of modified alkyl glucoside, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, glycerol polyoxyethylene ether, polyoxypropylene ether fatty acid ester, alkyl succinate sulfonate, naphthalenesulfonic acid formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, polymerized carboxylate and quaternary ammonium salt.

[0051] According to an embodiment of the present invention, the amino acids include: 2 to 4 parts by weight of glycine, 2 to 4 parts by weight of arginine, 2 to 4 parts by weight of tryptophan, and 0.5 to 1.5 parts by weight of glutamic acid. When the amino acids are in this ratio, the obtained biostimulant has a better effect of stimulating the growth of plant root hairs and the growth of plants or fruits.

[0052] According to an embodiment of the present invention, the peptide includes a plant-derived small peptide. The plant-derived small peptide is obtained by fermentation, and the content of fermentation metabolites is within a suitable range. In a preferred embodiment, the total amino acid content of the plant-derived small peptide is ≥80%, the organic matter content is >80%, and the free amino acid content is 1% to 10%. Free amino acids are more conducive to crop absorption, which is an important indicator for evaluating the quality of small peptide raw materials.

[0053] According to an embodiment of the present invention, the elemental fertilizer includes 10 to 20 parts by weight of monoammonium phosphate and 10 to 20 parts by weight of diammonium phosphate.

[0054] In a fifth aspect of the present invention, the present invention provides a method for promoting the growth of plant root hairs. According to an embodiment of the present invention, the method comprises: applying the nitrification inhibitor described in the first aspect, the nitrification inhibitor prepared by the method described in the second aspect, the biostimulant described in the third aspect, and / or the biostimulant prepared by the method described in the fourth aspect to the plant. Thus, compared with plants grown without the application of nitrification inhibitors and biostimulants, the root hairs of the plants of the present application grow faster.

[0055] It should be noted that the type of the plant is not particularly limited. As a preferred embodiment, the plant is a corn plant.

[0056] In the sixth aspect of the present invention, the present invention provides a method for promoting plant growth. According to an embodiment of the present invention, the method comprises: applying the nitrification inhibitor described in the first aspect, the nitrification inhibitor prepared by the method described in the second aspect, the biostimulant described in the third aspect, and / or the biostimulant prepared by the method described in the fourth aspect to the plant. Thus, compared with plants grown without the application of nitrification inhibitors and biostimulants, the plant of the present application grows and produces faster, and the plant yield is significantly improved.

[0057] It should be noted that the type of the plant is not particularly limited. As a preferred embodiment, the plant may be a corn plant.

[0058] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0059] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0060] Figure 1 This is a diagram showing the liquid chromatography test results of a nitrification inhibitor according to an embodiment of the present invention;

[0061] Figure 2 This is a diagram showing the liquid phase mass spectrometry detection result of a nitrification inhibitor according to an embodiment of the present invention. DETAILED DESCRIPTION

[0062] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0063] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" and "multiple" is at least two or two, such as two, two, three, three, etc., unless otherwise clearly and specifically defined.

[0064] In this document, the terms “include”, “have” or “comprises” are open expressions, that is, they include the contents specified in the present invention but do not exclude other contents.

[0065] As used herein, the term "optionally" generally means that the subsequently described event or circumstance may but need not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not.

[0066] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0067] Nitrification inhibitors show great potential in reducing nitrogen losses and improving nitrogen use efficiency in agricultural systems. Nitrification inhibitors work by inhibiting nitrification in the soil, thereby reducing nitrogen volatilization and loss, maintaining nitrogen supply in the soil to support crop growth.

[0068] In this article, 3,4-dimethylpyrazole, with the chemical formula C5H8N2O2P and the CAS number 202842-98-6, has specific applications in the chemical industry. For example, as a nitrification inhibitor, it can inhibit nitrification in the soil, reduce the volatilization and loss of nitrogen, and thus improve the utilization efficiency of nitrogen fertilizers. In addition, 3,4-dimethylpyrazole phosphate (DMPP), with the CAS number 202842-98-6, as a new type of nitrification inhibitor, has important application value in agriculture, helping to reduce nitrogen losses in agricultural systems and improve nitrogen utilization efficiency.

[0069] The embodiments of the present invention are described in detail below. It should be noted that the embodiments described below are exemplary and are only used to explain the present invention, and cannot be interpreted as limiting the present invention. In addition, if not explicitly stated, all reagents used in the following embodiments are commercially available, or can be synthesized according to this article or known methods, and the reaction conditions not listed are also easily available to those skilled in the art.

[0070] Example 1

[0071] The specific preparation method of nitrification inhibitors and biostimulants includes the following steps and links:

[0072] The culture medium is fermented by using Streptomyces, the preservation number of which is CGMCC No. 25834. The culture medium includes 3% soluble starch, 0.3% potassium nitrate, 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate, and 0.05% sodium chloride in a mass ratio. The culture medium is prepared by dissolving the above components in water and sterilizing at 120° C. The conditions during the fermentation process are: fermentation temperature of 35° C., shaking table speed of 250 pm, fermentation ventilation ratio of 1.5, and fermentation time of 75 hours.

[0073] First, 3,4-dimethylpyrazole was dissolved in an isopropanol organic solvent at a mass ratio of 1:3, and then 3,4-dimethylpyrazole and the fermentation metabolite of Streptomyces were mixed at a mass ratio of 1:3. The reaction temperature was 30°C and the reaction time was 4 hours to obtain a modified biological nitrification inhibitor DMPZ.

[0074] Then 37 parts by weight of modified biological nitrification inhibitor DMPZ are added into a reactor with a volume of 3kl, the temperature is controlled at 25°C, and then 20 parts by weight of monoammonium phosphate and 20 parts by weight of diammonium phosphate are added. The order of adding the two can be changed. After the monoammonium phosphate and diammonium phosphate are completely dissolved, 3 parts by weight of glycine, 3 parts by weight of arginine, 3 parts by weight of tryptophan, and 1 part by weight of glutamic acid are added. There is no special order for adding amino acids. After the amino acids are dissolved, 10 parts by weight of brown humic acid are added. After the amino acids are dissolved, 2 parts by weight of plant-derived small peptides (purchased from Jiangsu Hanling) are added. The total amino acid content of the plant-derived small peptides is ≥80%, the organic matter content is >80%, and the free amino acid content is 1% to 10%, wherein the organic matter includes total amino acids, and the total amino acids include free amino acids. Then 1 part by weight of an auxiliary agent is added, and the auxiliary agent is a modified alkyl glycoside. Finally, K(OH)2 is used to adjust the pH to 8.0, thereby obtaining a biostimulant.

[0075] Example 2

[0076] The specific preparation method of nitrification inhibitors and biostimulants includes the following steps and links:

[0077] The culture medium is fermented by using Streptomyces, the preservation number of which is CGMCC No.25834. The culture medium includes 0.5% soluble starch, 0.1% potassium nitrate, 0.01% potassium dihydrogen phosphate, 0.01% magnesium sulfate, and 0.01% sodium chloride in a mass ratio. The culture medium is prepared by dissolving the above components in water and sterilizing at 120°C. The conditions during the fermentation process are: fermentation temperature of 30°C, shaking speed of 150 pm, fermentation ventilation ratio of 1, and fermentation time of 70 hours.

[0078] First, 3,4-dimethylpyrazole was dissolved in an isopropanol organic solvent at a mass ratio of 1:5, and then 3,4-dimethylpyrazole and the fermentation metabolite of Streptomyces were mixed at a mass ratio of 1:3.2. The reaction temperature was 40°C and the reaction time was 6 hours to obtain the modified biological nitrification inhibitor DMPZ.

[0079] Then 47 parts by weight of modified biological nitrification inhibitor DMPZ are added into a reactor with a volume of 3kl, the temperature is controlled at 25°C, and then 15 parts by weight of monoammonium phosphate and 15 parts by weight of diammonium phosphate are added. The order of adding the two can be changed. After the monoammonium phosphate and diammonium phosphate are completely dissolved, 3 parts by weight of glycine, 3 parts by weight of arginine, 3 parts by weight of tryptophan, and 1 part by weight of glutamic acid are added. There is no special order for adding amino acids. After the amino acids are dissolved, 10 parts by weight of brown humic acid are added. After the amino acids are dissolved, 2 parts by weight of plant-derived small peptides (purchased from Jiangsu Hanling) are added. The total amino acid content of the plant-derived small peptides is ≥80%, the organic matter content is >80%, and the free amino acid content is 1% to 10%, wherein the organic matter includes total amino acids, and the total amino acids include free amino acids. Then 1 part by weight of an auxiliary agent is added, and the auxiliary agent is a modified alkyl glycoside. Finally, K(OH)2 is used to adjust the pH to 8.0, thereby obtaining a biostimulant.

[0080] Example 3

[0081] The specific preparation method of nitrification inhibitors and biostimulants includes the following steps and links:

[0082] The culture medium is fermented by using Streptomyces, the preservation number of which is CGMCC No. 25834. The culture medium includes soluble starch, 0.2% potassium nitrate, 0.03% potassium dihydrogen phosphate, 0.05% magnesium sulfate, and 0.04% sodium chloride in a mass ratio. The culture medium is prepared by dissolving the above components in water and sterilizing at 120° C. The conditions during the fermentation process are: fermentation temperature of 32° C., shaking table speed of 200 pm, fermentation ventilation ratio of 1.5, and fermentation time of 72 hours.

[0083] First, 3,4-dimethylpyrazole was dissolved in an isopropanol organic solvent at a mass ratio of 1:10, and then 3,4-dimethylpyrazole and the fermentation metabolite of Streptomyces were mixed at a mass ratio of 1:3.5. The reaction temperature was 50° C. and the reaction time was 10 h to obtain the modified biological nitrification inhibitor DMPZ.

[0084] Then 57 parts by weight of modified biological nitrification inhibitor DMPZ are added into a reactor with a volume of 3kl, the temperature is controlled at 25°C, and then 10 parts by weight of monoammonium phosphate and 10 parts by weight of diammonium phosphate are added. The order of adding the two can be changed. After the monoammonium phosphate and diammonium phosphate are completely dissolved, 3 parts by weight of glycine, 3 parts by weight of arginine, 3 parts by weight of tryptophan, and 1 part by weight of glutamic acid are added. There is no special order for adding amino acids. After the amino acids are dissolved, 10 parts by weight of brown humic acid are added. After it is dissolved, 2 parts by weight of plant-derived small peptides (purchased from Jiangsu Hanling) are added. The total amino acid content of the plant-derived small peptides is ≥80%, the organic matter content is >80%, and the free amino acid content is 1% to 10%, wherein the organic matter includes total amino acids, and the total amino acids include free amino acids. Then 1 part by weight of an auxiliary agent is added, and the auxiliary agent is a modified alkyl glycoside. Finally, K(OH)2 is used to adjust the pH to 8.0, thereby obtaining a biostimulant.

[0085] In addition, the inventors conducted high performance liquid chromatography and liquid chromatography-mass spectrometry on the modified biological nitrification inhibitor DMPZ obtained in the above Examples 1 to 3. The high performance liquid chromatography analysis conditions were as follows: chromatographic column: Thermo Acclaim C18 (4.6 mm id×250 mm, 5 μm), or a chromatographic column with equivalent effect; flow rate: 0.6 mL / min; column temperature: 30° C.; UV detector: detection wavelength, 230 nm; injection volume: 30 μL.

[0086] The elution gradient is shown in Table 1, and the mobile phase conditions are: mobile phase A: methanol; mobile phase B: 0.1‰ phosphoric acid aqueous solution.

[0087] Table 1: HPLC gradient elution conditions

[0088] Time (min) A(％) B(％) 0 10 90 5 10 90 10 90 10 18 90 10 20 10 90 30 10 90

[0089] Liquid phase mass spectrometry analysis conditions: (1) Chromatographic column: C18 reverse phase chromatographic column; column temperature: 30°C; mobile phase: A: methanol; B: 0.05% formic acid water; gradient elution conditions see Table 2.

[0090] Table 2: HPLC-MS gradient elution conditions

[0091] Time (min) A(％) B(％) 0 10.00 90.00 1.5 10.00 90.00 3.0 60.00 40.00 7.5 90.00 10.00 11.0 90.00 10.00 14.0 10.00 90.00 18.0 10.00 90.00

[0092] (2) Mass spectrometry conditions: Ms: drying gas temperature: 300°C; drying gas flow rate: 8 L / min; nebulizing gas pressure: 40 psi; sheath gas temperature: 350°C; sheath gas flow rate: 10 L / min; capillary voltage: 4000 V; nozzle voltage: 500 V; Framingtor: 100 V; acquisition range: 70-500.

[0093] Test result analysis:

[0094] The results of HPLC test were as follows Figure 1 As shown, under the same loading amount and the same liquid chromatography conditions, treatment 1 (3,4-dimethylpyrazole (DMP) only), treatment 2 (Streptomyces fermentation metabolites only), treatment 3 (3,4-dimethylpyrazole (DMP) + Streptomyces fermentation metabolites), and treatment 4 (3,4-dimethylpyrazole (DMP) reacts with Streptomyces fermentation metabolites) were set; at around 14 minutes, treatments 1, 3, and 4 all had characteristic peaks of DMP. It can be seen from the figure that the response values ​​of the characteristic peaks of treatments 1 and 3 are basically the same, indicating that the content of DMP is consistent. The response value of DMP in treatment 4 is relatively low, but a new peak appears at around 16 minutes to 17 minutes, indicating that the reaction of 3,4-dimethylpyrazole (DMP) + Streptomyces fermentation metabolites has formed a new substance.

[0095] Liquid phase mass spectrometry is performed on the new substances formed by the reaction. The liquid phase mass spectrometry detection results are as follows: Figure 2 As shown, the new substance peaked at 2.105 min, and the new peak in the mass spectrum showed a fragment ion peak with a mass-to-nuclear ratio of 158.97, which was the same fragment ion peak as 3,4-dimethylpyrazole phosphate, indicating that the new substance produced by the reaction was a new type of modified nitrification inhibitor similar to DMPP. The new substance was named DMPZ as mentioned above.

[0096] Comparative Example 1

[0097] The difference between this comparative example and Example 3 is:

[0098] The 57 parts by weight of the modified biological nitrification inhibitor DMPZ in Example 3 was replaced by 57 parts by weight of 3,4-dimethylpyrazole phosphate DMPP.

[0099] The rest of the experimental contents are consistent with those recorded in Example 3.

[0100] Comparative Example 2

[0101] The modified biological nitrification inhibitor DMPZ used in this comparative example was obtained by the same operation as in Example 3.

[0102] 91 parts by weight of modified biological nitrification inhibitor DMPZ are added into a reactor with a volume of 3kl, and the temperature is controlled at 25°C. Then, 2 parts by weight of monoammonium phosphate and 2 parts by weight of diammonium phosphate are added. The order of adding the two can be changed. After the monoammonium phosphate and diammonium phosphate are completely dissolved, 0.5 parts by weight of glycine, 0.5 parts by weight of arginine, 0.5 parts by weight of tryptophan, and 0.5 parts by weight of glutamic acid are added. There is no special order for adding amino acids. After the amino acids are dissolved, 1 part by weight of brown humic acid is added. After it is dissolved, 1 part by weight of plant-derived small peptide (purchased from Jiangsu Hanling) is added. The total amino acid content of the plant-derived small peptide is ≥80%, the organic matter content is >80%, and the free amino acid content is 1% to 10%, wherein the organic matter includes total amino acids, and the total amino acids include free amino acids. Then, 1 part by weight of an auxiliary agent is added, and the auxiliary agent is a modified alkyl glycoside. Finally, K(OH)2 is used to adjust the pH to 8.0, thereby obtaining a biostimulant.

[0103] Comparative Example 3

[0104] The modified biological nitrification inhibitor DMPZ used in this comparative example was obtained by the same operation as in Example 3.

[0105] 10 parts by weight of modified biological nitrification inhibitor DMPZ are added into a reactor with a volume of 3kl, and the temperature is controlled at 25°C. Then, 25 parts by weight of monoammonium phosphate and 25 parts by weight of diammonium phosphate are added. The order of adding the two can be changed. After the monoammonium phosphate and diammonium phosphate are completely dissolved, 7 parts by weight of glycine, 7 parts by weight of arginine, 8 parts by weight of tryptophan, and 5 parts by weight of glutamic acid are added. There is no special order for adding amino acids. After the amino acids are dissolved, 10 parts by weight of brown humic acid are added. After it is dissolved, 2 parts by weight of plant-derived small peptides (purchased from Jiangsu Hanling) are added. The total amino acid content of the plant-derived small peptides is ≥80%, the organic matter content is >80%, and the free amino acid content is 1% to 10%, wherein the organic matter includes total amino acids, and the total amino acids include free amino acids. Then, 1 part by weight of an auxiliary agent is added, and the auxiliary agent is a modified alkyl glycoside. Finally, K(OH)2 is used to adjust the pH to 8.0, thereby obtaining a biostimulant.

[0106] Comparative Example 4

[0107] The difference between this comparative example and Example 3 is:

[0108] 57 parts by weight of the modified biological nitrification inhibitor DMPZ in Example 3 were replaced by 57 parts by weight of Streptomyces fermentation metabolites.

[0109] The rest of the experimental contents are consistent with those recorded in Example 3.

[0110] Experimental testing:

[0111] The target biostimulants prepared in Examples 1 to 3 and Comparative Examples 1 to 4 were tested for specific time effects and the expression of root auxin-related genes ZmARF10, ZmYUC2, ZmYUC3, ZmYUC5, ZmPIN1 and ZmPIN9. The specific steps of the test are as follows:

[0112] 1. Test materials and sample collection

[0113] Test materials: Soil was collected in Meibu Town, Hedong, Linyi. The soil was sieved to remove large stones, and basic soil parameter data were collected (see Table 1). The corn variety used for the test was Denghai 602.

[0114] Table 1

[0115] Basic indicators of potting soil pH Organic matter Fast-Acting Potassium Alkaline nitrogen Available Phosphorus content 7.08 11.2 g / kg 64mg / kg 63mg / kg 5.7 mg / kg

[0116] The experiment used potted plants (polyethylene pots), each pot was filled with 20 kg of soil, one corn seedling per pot, and a base fertilizer of 15-15-15 compound fertilizer (purchased from Sinochem Shandong Fertilizer). Based on 150,000 kg of soil per mu of land, the amount of fertilizer applied was calculated based on the potting soil, 20 kg / mu, or 2.67 g per pot.

[0117] 280 pots of corn with consistent growth were selected as potted plants for the experiment, including 40 pots in the control group (Control, C); 40 pots in each of the treatment groups (Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4), and the target biostimulants of Examples 1, Example 2, Example 3, and Comparative Examples 1 to 4 were flushed during the growth period of the corn. The biostimulants were diluted 100 times and the flushing amount was 100 mL / pot.

[0118] Sample collection: On the 7th day after the target biostimulant was applied during the corn growth period, the roots of the same part of the corn (the root tip of the corn root system) were collected. Six corns were randomly collected from each group, and the relevant indicators were tested. The data of each group were expressed as average values.

[0119] 2. The determination methods of various indicators are as follows:

[0120] Standard biostimulants were applied during the growth period of corn. On the 7th day after the application, the growth indicators of corn plants were tested: fresh weight of the aboveground part; root growth and physiological indicators: fresh weight of the underground part, total number of root hairs, root hair density, root hair length, root auxin-related genes, etc. In addition, the yield of corn plants was tested at the harvest stage.

[0121] (1) Determination of root hair growth indexes: On the 7th day after treatment with each group of biostimulants, the corn plants were taken out of the polyethylene pots, the roots were rinsed with clean water, and the total number of root hairs, root hair density, root hair length, root hair starting distance and number of deformed root hairs of the seedling roots were observed using a stereo microscope.

[0122] (2) Determination of the total number of root hairs: On the 7th day after treatment with each group of biostimulants, the corn plants were removed from the polyethylene pots, the roots were rinsed with clean water, and four lateral roots were selected from the plants from top to bottom. Five fields of view were selected from each lateral root at an interval of 2 cm from bottom to top, and the field of view area was 1 cm 2 The number of root hairs in each field of view was observed using a stereo microscope, and the number was measured using ImageJ software after taking pictures. The average number of root hairs of the four lateral roots was the total number of root hairs for that treatment.

[0123] (3) Determination of root hair density: root hair density = total number of root hairs × magnification factor / magnified visual field area. On the 7th day after treatment with each group of biostimulants, the corn plants were taken out of the polyethylene pots and the roots were rinsed with clean water. The root hair density of each root was measured in 5 visual fields (1 cm 2 ) and the average value of the four roots was the root hair density of the plants in this treatment group.

[0124] (4) Determination of root hair length: On the 7th day after treatment with each group of biostimulants, the corn plants were removed from the polyethylene pots, the roots were rinsed with clean water, and four lateral roots were randomly selected from top to bottom. Ten root hairs were randomly measured for each lateral root. After taking pictures under a stereo microscope, the root hair length was measured using ImageJ software. The average value of the four lateral roots was the root hair length of the treatment.

[0125] (5) Determination of auxin gene expression: ① Extraction of sample RNA: Use RNA extraction kit BOXBIO AKNA001 total RNA extraction detection kit to extract total RNA of corn root system according to the manufacturer's operating procedures. The extracted total RNA was immediately reverse transcribed. ② Fluorescence quantitative PCR to detect the transcription level of auxin-related genes, design primers: According to the conservative sequence of the coding region of the auxin-related gene to be detected, primers were designed using NCBI software, and 18SsRNA (AF168884.4) was used as the internal reference gene (the sequences of each primer are shown in Table 2). ③ RNA reverse transcription: Use the reverse transcription kit PrimeScriptTM RT reagentKit with gDNA Eraser (Takara) to synthesize the first-strand cDNA. ④ Real-time fluorescence quantitative RT-PCR: Using the reverse transcribed cDNA product as a template, the fluorescence quantitative kit SYBR Premix EX TapTM (Perfect Real Time) was used to detect the transcription level of auxin-related genes. The results are shown in Table 3.

[0126] Instruments used: High performance liquid chromatography (Thermo Fisher), liquid chromatography-mass spectrometry (Agilent), real-time fluorescence quantitative PCR instrument, etc.

[0127] The primers were synthesized by Sangon Biotechnology (Shanghai) Co., Ltd.

[0128] Table 2: Primer information of auxin-related genes

[0129]

[0130]

[0131] 3. Result analysis:

[0132] As shown in Table 3, the expression levels of auxin-related genes ZmARF10, ZmYUC2, ZmYUC3, ZmYUC5, ZmPIN1, and ZmPIN9 in the root system of corns to which the biostimulants in Examples 1 to 3 were applied were upregulated compared with the control group and each group of Examples 1 to 3, indicating that the biostimulants provided in Examples 1 to 3 of the present invention can promote the expression of auxin-related genes in the root system of corns. Thereby promoting the synthesis and transport of root auxin, promoting the growth of root hairs, and promoting the growth and development of the root system.

[0133] Table 3: Relative expression levels of auxin-related genes in the roots of plants in different treatment groups

[0134] Zmactin ZmARF10 ZmYUC2 ZmYUC3 ZmYUC5 Zmpin1 Zmpin9 Control group 0.8 1.1 2.1 2.4 2.2 3.3 3.2 Example 1 1.0 7.6 10.3 11.6 12.3 9.3 8.7 Example 2 1.2 8.4 12.3 14.4 13.5 10.3 10.6 Example 3 1.3 10.2 14.2 15.9 14.2 13.0 12.8 Comparative Example 1 0.8 3.2 4.3 4.8 5.3 4.7 6.5 Comparative Example 2 0.9 4.0 3.9 5.0 5.2 5.5 4.9 Comparative Example 3 0.8 3.9 5.1 4.9 5.7 6.0 5.4 Comparative Example 4 0.8 3.1 4.0 4.6 5.0 4.8 5.3

[0135] From the results shown in Table 4, it can be seen that the treatment groups of different embodiments all have root-promoting effects, but the changes in the proportions of the components also have different effects on root-promoting effects. The compounding of amino acids, humic acid, nitrogen, phosphorus and potassium with modified biological nitrification inhibitors has an obvious function of promoting crop root hairs. Compared with the results of Comparative Examples 1 to 3, it is shown that the modified biological nitrification inhibitor has a better effect of promoting root hairs, and the functional effect of enhancing the growth of root hairs is more obvious.

[0136] After 7 days of treatment with the target biostimulant in different embodiments, the number, length and density of root hairs of the corn root system can be increased, the activity of the root system can be improved, the absorption of crop nutrients can be promoted, and the growth indicators of the aboveground and underground parts of the corn plants can be improved. In addition, the target biostimulant can increase the yield of mature corn plants after treatment, indicating that the target biostimulant improves the ability of the root system to absorb nutrients by promoting the growth of root hairs, thereby promoting crop yield.

[0137] Comprehensive analysis of the above indicators showed that the target biostimulants of Examples 1 to 3 promoted the improvement of indicators such as the number, length, and density of root hairs of crops, thereby improving crop-related growth indicators and the yield of mature crops.

[0138] Conclusion: The biostimulant of the present invention is obtained by compounding amino acids, humic acid, nitrogen, phosphorus and potassium with modified biological nitrification inhibitors. It can target and increase the expression levels of crop root auxin-related genes ZmARF10, ZmYUC2, ZmYUC3, ZmYUC5, ZmPIN1, and ZmPIN9, promote root hair growth, enhance root vitality, promote root nutrient absorption, promote crop growth, and promote plant material accumulation, and has a significant yield-increasing effect.

[0139]

[0140] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0141] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A nitrification inhibitor, characterized in that: The invention comprises 3,4-dimethylpyrazole and streptomyces fermentation liquid in a weight ratio of 1:(2.5-4.0), wherein the streptomyces fermentation liquid contains organic acid.

2. The nitrification inhibitor according to claim 1, characterized in that The Streptomyces fermentation broth is obtained from a Streptomyces fermentation medium, and the Streptomyces was deposited in the China Center for Type Culture Collection on September 28, 2022, with a deposit number of CGMCC No. 25834; Optionally, the culture medium comprises 0.5% to 3% by mass of soluble starch, 0.1% to 0.3% by mass of potassium nitrate, 0.01% to 0.05% by mass of potassium dihydrogen phosphate, 0.01% to 0.05% by mass of magnesium sulfate and 0.01% to 0.05% by mass of sodium chloride; Optionally, the fermentation broth is obtained by fermenting for 70 to 75 hours at 30° C. to 35° C., a shaking speed of 150 rpm to 250 rpm, and an aeration ratio of 1 to 1.5; Optionally, the 3,4-dimethylpyrazole is preliminarily dissolved in an organic solvent at a mass ratio of 1:(2-12); Optionally, the organic solvent comprises at least one of the following: methanol, ethanol, isopropanol and n-butanol; Optionally, the organic solvent is isopropanol.

3. A method for preparing the nitrification inhibitor according to claim 1 or 2, characterized in that: The method comprises: mixing 3,4-dimethylpyrazole and streptomyces fermentation liquid at a weight ratio of 1:(2.5-4.0) to obtain the nitrification inhibitor.

4. The method according to claim 3, characterized in that The fermentation liquid is obtained by fermenting Streptomyces in a culture medium, wherein the culture medium comprises 0.5% to 3% by mass of soluble starch, 0.1% to 0.3% by mass of potassium nitrate, 0.01% to 0.05% by mass of potassium dihydrogen phosphate, 0.01% to 0.05% by mass of magnesium sulfate and 0.01% to 0.05% by mass of sodium chloride; Optionally, the Streptomyces was deposited in China Center for Type Culture Collection on September 28, 2022, with a deposit number of CGMCC No.25834; Optionally, the fermentation is carried out at 30°C to 35°C, a shaking speed of 150 rpm to 250 rpm, and an aeration ratio of 1 to 1.5 for 70 to 75 hours.

5. The method according to claim 3, characterized in that: The 3,4-dimethylpyrazole is dissolved in an organic solvent in advance at a mass ratio of 1:(2-12); Optionally, the organic solvent comprises at least one of the following: methanol, ethanol, isopropanol and n-butanol; Optionally, the organic solvent is isopropanol; Optionally, the temperature of the mixed reaction is 25°C to 55°C; Optionally, the mixing reaction time is 3 to 12 hours.

6. A biostimulant, characterized in that: include: 23 to 68 parts by weight of the nitrification inhibitor according to claim 1 or 2.

7. The biostimulant according to claim 6, characterized in that The biostimulant further comprises: 5 to 15 parts by weight of amino acids, 1 to 10 parts by weight of peptides, 5 to 10 parts by weight of humic acid, and 20 to 40 parts by weight of elemental fertilizers; Optionally, the amino acid comprises at least one of tryptophan, glycine, arginine, glutamic acid and phenylalanine; Optionally, the peptide comprises at least one of a plant-derived small peptide, a plant-derived polypeptide, an animal-derived small peptide, and an animal-derived polypeptide; Optionally, the humic acid comprises at least one of sodium phytate, potassium humate, potassium fulvic acid, palmitic acid, fulvic acid, black fulvic acid, biochemical fulvic acid and brown-yellow fulvic acid; Optionally, the elemental fertilizer includes at least one of urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, ammonium polyphosphate, urea-formaldehyde and urea phosphate.

8. The biostimulant according to claim 6, characterized in that Also includes: 1 to 2 parts by weight of an auxiliary agent; Optionally, the auxiliary agent includes: at least one of modified alkyl glycoside, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, glycerol polyoxyethylene ether polyoxypropylene ether fatty acid ester, alkyl succinate sulfonate, naphthalenesulfonic acid formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, polymerized carboxylate and quaternary ammonium salt.

9. The biostimulant according to claim 7, characterized in that: The amino acids include: 2 to 4 parts by weight of glycine, 2 to 4 parts by weight of arginine, 2 to 4 parts by weight of tryptophan, and 0.5 to 1.5 parts by weight of glutamic acid; Optionally, the peptide comprises a plant-derived small peptide; Optionally, the elemental fertilizer includes 10 to 20 parts by weight of monoammonium phosphate and 10 to 20 parts by weight of diammonium phosphate.

10. A method for preparing the biostimulant according to any one of claims 5 to 8, characterized in that: include: (1) The nitrification inhibitor according to claim 1 or 2 is subjected to a first mixing treatment with an elemental fertilizer.

11. The method according to claim 10, characterized in that The method further comprises: (2) subjecting the first mixed treatment product to a second mixing treatment with an amino acid, wherein the second mixing treatment is performed until the amino acid is completely dissolved; (3) performing a third mixing treatment on the second mixed treatment product and humic acid, wherein the third mixing treatment is performed until the humic acid is completely dissolved; (4) subjecting the third mixed treatment product to a fourth mixed treatment with a peptide to obtain the biostimulant.

12. The method according to claim 11, characterized in that The first, second, third and fourth mixing treatments are carried out at 20°C to 30°C; Optionally, the method further comprises: performing a fifth mixing treatment on the fourth mixing treatment product and an auxiliary agent; Optionally, the method further comprises: adjusting the pH of the fifth mixed treatment product to 7.5 to 8.5; Optionally, the amino acid comprises at least one of tryptophan, glycine, arginine, glutamic acid and phenylalanine; Optionally, the peptide comprises at least one of a plant-derived small peptide, a plant-derived polypeptide, an animal-derived small peptide, and an animal-derived polypeptide; Optionally, the humic acid comprises at least one of sodium humate, potassium humate, potassium fulvic acid, palmitic acid, fulvic acid, black humic acid, biochemical fulvic acid and brown-yellow fulvic acid; Optionally, the elemental fertilizer includes at least one of urea, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, ammonium polyphosphate, urea-formaldehyde and urea phosphate.

13. The method according to claim 12, characterized in that The auxiliary agent includes: at least one of modified alkyl polyglycoside, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, glycerol polyoxyethylene ether, polyoxypropylene ether fatty acid ester, alkyl succinate sulfonate, naphthalenesulfonic acid formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, polymeric carboxylate and quaternary ammonium salt; Optionally, the amino acids include: 2 to 4 parts by weight of glycine, 2 to 4 parts by weight of arginine, 2 to 4 parts by weight of tryptophan, and 0.5 to 1.5 parts by weight of glutamic acid; Optionally, the peptide comprises a plant-derived small peptide; Optionally, the elemental fertilizer includes 10 to 20 parts by weight of monoammonium phosphate and 10 to 20 parts by weight of diammonium phosphate.

14. A method for promoting the growth of plant root hairs, characterized in that: include: The nitrification inhibitor according to claim 1 or 2, the nitrification inhibitor prepared by the method according to any one of claims 3 to 5, the biostimulant according to any one of claims 6 to 9, and / or the biostimulant prepared by the method according to any one of claims 10 to 13 are applied to the plants.

15. A method for promoting plant growth, characterized in that: include: The nitrification inhibitor according to claim 1 or 2, the nitrification inhibitor prepared by the method according to any one of claims 3 to 5, the biostimulant according to any one of claims 6 to 9, and / or the biostimulant prepared by the method according to any one of claims 10 to 13 are applied to the plants.

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