The invention relates to a taste cell sensor using a surface acoustic wave resonator. The taste cell sensor includes the surface acoustic wave resonator, which is encapsulated in a metal shell by vacuum. The surface acoustic wave resonator adopts ST cut quartz as a piezoelectric substrate material, is prepared by a photolithography process, the emitted surface acoustic frequency is 433MHZ. The taste cell sensor also includes a silk-screen printing carbon electrode, which is in series connection with the surface acoustic wave resonator. The silk-screen printing carbon electrode is firstly modified by amino acid and then coated with an NCI-H716 cell suspension solution. The taste cell sensor provided by the invention has the advantages of small volume, practicability and convenience, fast electrode response, high sensitivity, good batch repeatability, low cost and the like, can achieve high frequency, and can realize rapid real-time detection. The preparation method has the characteristics of simple preparation process, small material consumption, high process repeatability and controllability, and is convenient for batch production.