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Taste cell sensor using surface acoustic wave resonator and preparation method thereof

A surface acoustic wave and resonator technology, applied in the field of cell sensors, can solve the problems of high price, difficult to meet rapid detection, large size of cell sensing detection system, etc., and achieve low cost, high repeatability and controllability, Good batch repeatability

Inactive Publication Date: 2014-10-08
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a surface acoustic wave resonator with high sensitivity, low cost and fast electrode response in order to solve the defects that the existing cell sensing detection system is large in size and expensive, and it is difficult to meet the needs of on-site rapid detection. taste cell sensor

Method used

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  • Taste cell sensor using surface acoustic wave resonator and preparation method thereof
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  • Taste cell sensor using surface acoustic wave resonator and preparation method thereof

Examples

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Effect test

Embodiment 1

[0034] A preparation method of a taste cell sensor using a surface acoustic wave resonator, the preparation method comprising the following steps:

[0035] a) Pre-treatment: Dry the screen-printed carbon electrode at 45°C for 10 hours, then ultrasonically clean it with acetone, soak it in ultrapure water for 5 minutes, irradiate it with ultraviolet rays for 25 minutes, and dry it naturally for use; ultrasonically disperse the graphene in deionized water , to obtain a 0.3g / L suspension, evenly drop-coat the suspension on the working electrode area of ​​the above-mentioned screen-printed carbon electrode, dry it at 50°C, and then drop-coat 100μg / ml polylysine solution on the The working electrode area of ​​the graphene-coated screen-printed carbon electrode was left overnight at room temperature, washed 4 times with ultrapure water the next day, and dried for use;

[0036] b) NCI-H716 cell culture: NCI-H716 cell culture medium is 10% fetal bovine serum, penicillin and strept...

Embodiment 2

[0039] A preparation method of a taste cell sensor using a surface acoustic wave resonator, the preparation method comprising the following steps:

[0040] a) Pre-treatment: Dry the screen-printed carbon electrode at 50°C for 12 hours, then ultrasonically clean it with absolute ethanol, soak it in ultrapure water for 8 minutes, irradiate it with ultraviolet light for 30 minutes, and then dry it naturally; In deionized water, a 0.5g / L suspension was obtained, and the suspension was uniformly drip-coated on the working electrode area of ​​the above-mentioned screen-printed carbon electrode, dried at 65°C, and then a 100μg / ml serine solution was drip-coated on the coating The working electrode area of ​​the graphene-coated screen-printed carbon electrode was left overnight at room temperature, washed 6 times with ultrapure water the next day, and dried for later use;

[0041] b) NCI-H716 cell culture: NCI-H716 cell culture medium is 10% fetal bovine serum, penicillin and streptom...

Embodiment 3

[0044] A preparation method of a taste cell sensor using a surface acoustic wave resonator as claimed in claim 1, wherein the preparation method comprises the following steps:

[0045] a) Pretreatment: Dry the screen-printed carbon electrode at 60°C for 14 hours, then ultrasonically clean it with acetone, then soak it in ultrapure water for 10 minutes, irradiate it with ultraviolet rays for 35 minutes, and then dry it naturally for use; ultrasonically disperse the graphene in deionized water , to obtain a 1g / L suspension, evenly drop-coat the suspension on the working electrode area of ​​the above-mentioned screen-printed carbon electrode, dry it at 50-80°C, and then drop-coat the 100μg / ml arginine solution on the coated The working electrode area of ​​the graphene-coated screen-printed carbon electrode was left overnight at room temperature, washed 10 times with ultrapure water the next day, and dried for use;

[0046] b) NCI-H716 cell culture: NCI-H716 cell culture medium is...

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Abstract

The invention relates to a taste cell sensor using a surface acoustic wave resonator. The taste cell sensor includes the surface acoustic wave resonator, which is encapsulated in a metal shell by vacuum. The surface acoustic wave resonator adopts ST cut quartz as a piezoelectric substrate material, is prepared by a photolithography process, the emitted surface acoustic frequency is 433MHZ. The taste cell sensor also includes a silk-screen printing carbon electrode, which is in series connection with the surface acoustic wave resonator. The silk-screen printing carbon electrode is firstly modified by amino acid and then coated with an NCI-H716 cell suspension solution. The taste cell sensor provided by the invention has the advantages of small volume, practicability and convenience, fast electrode response, high sensitivity, good batch repeatability, low cost and the like, can achieve high frequency, and can realize rapid real-time detection. The preparation method has the characteristics of simple preparation process, small material consumption, high process repeatability and controllability, and is convenient for batch production.

Description

technical field [0001] The invention relates to a cell sensor, in particular to a taste cell sensor using a surface acoustic wave resonator with high sensitivity, low cost and fast electrode response and a preparation method thereof. [0002] Background technique [0003] Cell sensors have become one of the hot spots in the field of biosensing. After years of development, cell sensors have been widely used in many fields. At present, cell sensors mainly include microelectrode array (MEA) cell sensors, field effect transistor (FET) cell sensors, light addressable potential cell sensors (LAPS), patch clamp cell sensors[12,13], immune cell sensors, etc. . However, the electrodes used in the above technologies are generally more expensive, and most of them are disposable, so the detection cost is relatively high. Screen-printed electrode is a common biological and chemical compatible electrode, which has the advantages of low cost, easy processing, disposable use, etc. Cells,...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N29/00
Inventor 惠国华黄洁杜佳苏郑海霞
Owner ZHEJIANG GONGSHANG UNIVERSITY
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