Culture medium for separating brucella in marmot tissues and preparation method thereof

A technology of Brucella and culture medium, applied in the field of microorganisms, can solve the problems of time-consuming, inability to detect positive separation of brucellosis antibodies, difficulty in separation and purification, etc., and achieve the effects of simple preparation, stable quality, and high number of cultured bacteria.

Pending Publication Date: 2021-07-16
青海省地方病预防控制所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this culture medium is suitable for non-polluted samples, and it is difficult and time-consuming to isolate and purify Brucella from polluted and tissue samples. It is an urgent problem to be solved to improve the traditional culture medium and methods for suitable and efficient culture medium for bacteria.
The applicant first isolated Brucella from the tissue samples of the Himalayan m...

Method used

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  • Culture medium for separating brucella in marmot tissues and preparation method thereof
  • Culture medium for separating brucella in marmot tissues and preparation method thereof
  • Culture medium for separating brucella in marmot tissues and preparation method thereof

Examples

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Embodiment 1

[0060] This embodiment provides a culture medium for isolating Brucella in marmot tissue, and using the culture medium to culture the Brucella isolated in Himalayan woodchuck tissue.

[0061] A kind of culture medium that is used for isolating Brucella in marmot tissue, prepares 1000mL of this culture medium, specifically is made of following component: agar 13g; Trypsin 10g; Sodium chloride 5g; Anhydrous sodium sulfite 0.1g ;yeast extract 3g; bovine liver extract powder 5g; D-glucose 2g; erythritol 0.1g; sodium citrate 0.2g; premium horse serum 6ml; bacitracin 15 units / ml; ; The rest of the distilled water.

[0062] Above-mentioned a kind of preparation method that is used to isolate the culture medium of Brucella in marmot tissue, specifically comprises the steps:

[0063] Step 1: Weigh 13g of agar, 10g of trypsin, 5g of sodium chloride, 0.1g of anhydrous sodium sulfite, 3g of yeast extract, and 5g of bovine liver extract powder in the above proportions and add them into ...

Embodiment 2

[0072] This embodiment provides a culture medium for isolating Brucella in marmot tissue, and using the culture medium to culture the Brucella isolated in Himalayan woodchuck tissue.

[0073] A kind of culture medium that is used for isolating Brucella in marmot tissue, prepares 1000mL of this culture medium, specifically is made of following components: agar 18g; Trypsin 15g; Sodium chloride 8g; Anhydrous sodium sulfite 0.4g ; Yeast extract 5g; Beef liver extract powder 13g; D-glucose 5g; Erythritol 0.2g; Sodium citrate 0.5g; Premium horse serum 8ml; Bacitracin 23 units / ml; ; The rest of the distilled water.

[0074] Above-mentioned a kind of preparation method that is used to isolate the culture medium of Brucella in marmot tissue, specifically comprises the steps:

[0075] Step 1: Weigh 18g of agar, 15g of trypsin, 8g of sodium chloride, 0.4g of anhydrous sodium sulfite, 5g of yeast extract, and 13g of bovine liver extract powder in the above ratio and add them into dist...

Embodiment 3

[0084] This embodiment provides a culture medium for isolating Brucella in marmot tissue, and using the culture medium to culture the Brucella isolated in Himalayan woodchuck tissue.

[0085] A kind of substratum that is used to isolate Brucella in marmot tissue, prepares this substratum of 1000mL, specifically is made of following component:

[0086] Agar 15g; Trypsin 18g; Sodium chloride 4g; Anhydrous sodium sulfite 0.2g; Yeast extract 2g; Premium horse serum 10ml; bacitracin 25 units / ml; cycloheximide 100ug / ml; the rest double distilled water.

[0087] Above-mentioned a kind of preparation method that is used to isolate the culture medium of Brucella in marmot tissue, specifically comprises the steps:

[0088] Step 1: Weigh 15g of agar, 18g of trypsin, 4g of sodium chloride, 0.2g of anhydrous sodium sulfite, 2g of yeast extract, and 15g of bovine liver extract powder in the above proportions and add them into double distilled water and mix to 1000mL to obtain a mixture; ...

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Abstract

The invention discloses a culture medium for separating brucella in marmot tissues and a preparation method thereof. The culture medium is specifically composed of components: agar, tryptose, sodium chloride, anhydrous sodium sulfite, a yeast extract, bovine liver extract powder, D-glucose, erythritol, sodium citrate, special-grade horse serum, bacitracin, actinomycete ketone and distilled water or double distilled water. The culture medium is a solid culture medium capable of culturing brucella and is balanced in nutrition; the added components not only meet a carbon source, a nitrogen source and the like required by the metabolism of the brucella, but also inhibit the growth of other bacteria in a polluted sample, so that the detection rate of the brucella in the polluted sample is improved; all the components supplement each other to achieve a synergistic effect; and the culture medium provided by the invention can meet the growth and propagation requirements of separating brucella from marmot himalayan tissues, and has the advantages of stable quality, simplicity and convenience in preparation and high number of cultured bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for isolating Brucella in marmot tissues and a preparation method thereof. Background technique [0002] Brucellosis (referred to as brucellosis) is a zoonotic disease caused by Brucella, which is listed as one of the legally notifiable infectious diseases by the World Health Organization (WHO) and the World Organization for Animal Health (OIE) , my country listed it as a Class B infectious disease. Brucella is an intracellular parasitic aerobic Gram-negative bacterium, which mostly parasitizes the reticuloendothelial system and can survive in humans and animals. Its reproduction and growth have high nutritional requirements and slow growth. It grows best in an environment of 37°C and pH 6.6-7.4. The pathogens of brucellosis in Qinghai Province are divided into three species and six biotypes, mainly Brucella melis. There is a phenomenon of transfer betwee...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/20C12R1/01
CPCC12N1/02C12N1/20
Inventor 薛红梅谢辉李积权马丽杨旭欣吴海生王建玲徐立青张爱萍赵元博赵志军于守鸿
Owner 青海省地方病预防控制所
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