Culture medium for separating brucella in marmot tissues and preparation method thereof

Abstract

The invention discloses a culture medium for separating brucella in marmot tissues and a preparation method thereof. The culture medium is specifically composed of components: agar, tryptose, sodium chloride, anhydrous sodium sulfite, a yeast extract, bovine liver extract powder, D-glucose, erythritol, sodium citrate, special-grade horse serum, bacitracin, actinomycete ketone and distilled water or double distilled water. The culture medium is a solid culture medium capable of culturing brucella and is balanced in nutrition; the added components not only meet a carbon source, a nitrogen source and the like required by the metabolism of the brucella, but also inhibit the growth of other bacteria in a polluted sample, so that the detection rate of the brucella in the polluted sample is improved; all the components supplement each other to achieve a synergistic effect; and the culture medium provided by the invention can meet the growth and propagation requirements of separating brucella from marmot himalayan tissues, and has the advantages of stable quality, simplicity and convenience in preparation and high number of cultured bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for isolating Brucella in marmot tissues and a preparation method thereof. Background technique [0002] Brucellosis (referred to as brucellosis) is a zoonotic disease caused by Brucella, which is listed as one of the legally notifiable infectious diseases by the World Health Organization (WHO) and the World Organization for Animal Health (OIE) , my country listed it as a Class B infectious disease. Brucella is an intracellular parasitic aerobic Gram-negative bacterium, which mostly parasitizes the reticuloendothelial system and can survive in humans and animals. Its reproduction and growth have high nutritional requirements and slow growth. It grows best in an environment of 37°C and pH 6.6-7.4. The pathogens of brucellosis in Qinghai Province are divided into three species and six biotypes, mainly Brucella melis. There is a phenomenon of transfer betwee...

AI Technical Summary

Problems solved by technology

However, this culture medium is suitable for non-polluted samples, and it is difficult and time-consuming to isolate and purify Brucella from polluted and tissue samples. It is an urgent problem to be solved to improve the traditional culture medium and methods for suitable and efficient culture medium for bacteria.

The applicant first isolated Brucella from the tissue samples of the Himalayan marmot. The existing technology cannot realize the detection of brucellosis antibody positive in the Himalayan marmot while isolating Brucella. In the actual operation of isolating Brucella in China, because the samples are often contaminated and mixed with various other pathogenic microorganisms, it is necessary to select a medium with excellent sensitivity for the cultivation and isolation of Brucella

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