A strain of Acetobacter gluconicum and screen purification method thereof

A technology of Gluconacetobacter and purification method, which is applied in the field of screening and purification of a strain of Gluconacetobacter, which can solve the problems of retention theory research, cellulose-producing bacteria classification confusion, etc.

Inactive Publication Date: 2009-08-12
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the classification of cellulose-producing bacteria in my country is very confusing, and the old nomenclature is still use

Method used

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  • A strain of Acetobacter gluconicum and screen purification method thereof
  • A strain of Acetobacter gluconicum and screen purification method thereof
  • A strain of Acetobacter gluconicum and screen purification method thereof

Examples

Experimental program
Comparison scheme
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Embodiment

[0026] The screening purification method of Gluconacetobacter bacterial strain comprises the following steps:

[0027] (1) Enrichment culture

[0028] Take the damaged part of the defective fruit, inoculate it into the enrichment culture concentrate containing 50mg / L cycloheximide and 5% ethanol, put it in a 30°C constant temperature incubator and cultivate it for 5 days, and pick out the condensate that appears during the cultivation process in time. Adhesive film, transfer to sterilized physiological saline and extract for 1 day;

[0029] (2) flat plate coating

[0030] Gradiently dilute the extract obtained in step (1) and spread it on a BSH solid plate, and culture it in a constant temperature incubator at 30°C for 5 days, pick a single colony and re-introduce it into the BSH liquid medium;

[0031] (3) Preliminary screening: Take out the film produced in step (2) and soak it in sterilized normal saline for 1 day, take the extract and culture it on a plate for 5 days, an...

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Abstract

The invention discloses a gluconoacetobacter sp. strain and a screening and purifying method. The screening and purifying method is characterized in that: defective fruits are used as raw materials; cycloheximide is used to suppress the growth of molds and other fungi, and plate enrichment cultivation, gradient dilution plate coating, primary screening, second screening and other methods are adopted for screening and purification to obtain the strain generating bacteria cellulose. According to the description in the ninth version of Bergey's Manual of Determinative Bacteriology, the strain is determined as a novel strain by the analysis of physiological and biochemical properties and 16 Sr DNA sequence. The invention strain obtained by the method has a wide selection range of carbon sources and nitrogen sources, a maximum yield of bacteria cellulose at a pH value of 6.5 and normal temperature, and a higher yield than that of common cellulose-generating bacteria, and has better implementation prospect in the industrial production of bacteria cellulose.

Description

technical field [0001] The invention relates to a strain of Gluconacetobacter and a screening and purifying method. [0002] The bacterial strain obtained in the present invention has been preserved in China Center for Type Culture Collection on November 21, 2008, number: CCTCC NO: M208234, name: Gluconacetobacter sp. G-29 (Gluconacetobacter sp.). [0003] Gluconacetobacter G-29 was isolated from Prunus spp., and was named G-29 according to the name of the sample source from which the strain was isolated and the number of the strain. Background technique [0004] Regarding bacterial cellulose, there have been 120 years of research history in foreign countries, while China only started in the 1980s. Bacterial cellulose, like plant cellulose, is a macromolecule composed of glucose groups connected by β-1,4-glycosidic bonds. Plant cellulose is an important production raw material in industries such as papermaking, textiles, biodegradable materials, medicine, and food additive...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12R1/01
Inventor 胡承曹海鹏袁帅赵昆
Owner SICHUAN UNIV
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