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Coating agent for seed and its preparing process and use

A technology of coating agent and seed, which is applied in the field of agriculture, can solve the problems that there are no registered products for the seed coating agent with the function of repelling rodents, and no active ingredients of actinone compounds have been found, so as to achieve a good effect of repelling rodents

Inactive Publication Date: 2006-10-11
SHANGHAI PESTICIDE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] But all at home and abroad as seed coating agent to prevent and treat disease, worm, weed damage and increase nutrition as main, the seed coating agent with rodent repelling function all does not have registered product at home and abroad, also does not yet see simultaneously and utilizes phenotype compound as yet Report on the Active Components of Seed Coating Agent

Method used

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  • Coating agent for seed and its preparing process and use
  • Coating agent for seed and its preparing process and use
  • Coating agent for seed and its preparing process and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: microbial fermentation

[0035] The seed culture medium is formulated with 2% glucose, 1% starch, 2% soybean cake powder, 0.2% peptone, 0.2% ammonium sulfate, 0.2% sodium chloride, 0.02% potassium dihydrogen phosphate, and 0.05% magnesium sulfate, pH Adjust to 7.2, disinfect and sterilize. The strain SPRI-201 Streptomyces genius CGMCC No.1317 was inoculated in the culture medium, and cultured at 28°C for 36-40 hours; the culture was used as a seed, and inoculated at a ratio of 5-20% in 4% glucose, 4% starch, 2% soybean cake powder, 0.2% peptone, 0.2% ammonium sulfate, 0.2% sodium chloride, 0.02% potassium dihydrogen phosphate, 0.05% magnesium sulfate, and 0.6% calcium carbonate. , 28 ℃ ventilation and stirring culture for 90-96 hours.

[0036] 2. Extraction

Embodiment 2

[0037] Embodiment 2: Separation and extraction of active ingredients

[0038] Get 100 kilograms of fermented culture fluid that embodiment 1 obtains, filter, get 80 kilograms of filtrates, filtrate passes through 5 kilograms of pre-treated 1300 type macroporous adsorption resins, washes resin with 5 kilograms of 65% acetone aqueous liquid after washing, decompression decompression Remove the acetone in the analysis solution to obtain 500 grams of tan liquid (I); extract twice with 400 milliliters of chloroform, and concentrate the chloroform layer to obtain 150 grams of yellow oil (II); Crystallize to obtain 80 grams of light yellow crystals; recrystallize the crude crystals in ethanol to obtain 60 grams of white crystals.

[0039] 3. Structural Determination of Active Ingredients

Embodiment 3

[0040] Embodiment 3: Determination of basic physical and chemical constants

[0041] Get the white crystalline compound that embodiment 2 obtains, measure its melting point and solubility, the results are as follows:

[0042] Appearance: white powder crystal

[0043] Melting point: 119-121°C

[0044] Solubility: easily soluble in chloroform, ethyl acetate, acetone and other organic solvents, soluble in methanol, ethanol, acetonitrile, slightly soluble in water, etc.;

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Abstract

The invention provides a seed coating agent using cycloheximide as the effective composition, which is prepared through isolating and extracting active ingredient from the microbial cultivation substance of SPRI-201 (Streptomyces genius) CGMCC No.1317, then mixing with carrying agent and auxiliary agent.

Description

technical field [0001] The invention belongs to the field of agriculture, and specifically relates to a seed coating agent with cycloheximide as an active ingredient, a preparation method thereof, and an application of the seed coating agent. Background technique [0002] Cyclonone compounds can be produced by various microorganisms, for example, strains such as Streptomyces gray albicans ATCC12757 and Stroptomycese naraenis described in US Patent No. 3915803 can produce such compounds. This type of compound was developed in the 1960s for the control of fungal diseases of crops, and in the 1970s for rodent-proof cables. The patents for adding components to anti-rodent cables are mainly patents applied by some companies in Japan and the United States in 1980. In 1991, Tanabe Seiyaku Co., Ltd. of Japan applied for a microcapsule material containing cycloheximide with anti-rodent effect The US patent (5,002,768) of the manufacturing method, and the later patents on cycloheximi...

Claims

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Application Information

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IPC IPC(8): A01N43/40A01C1/06
Inventor 徐文平杨代斌薛章荣陶黎明郭磊旷文丰杨星陆迪生郑佳玲顾学斌王磊张育雷庞鸿昌
Owner SHANGHAI PESTICIDE RES INST
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