Pediococcus pentosaceus strain P9-5, and screening identification and application thereof

A technology of Pediococcus pentosaceae and a screening method, which is applied in the detection and application fields of microorganisms, can solve the problems of not developing shaped and economical biological preparation products, achieve reliable and effective detection methods, improve the level of prevention and control, and reduce import and export risk effect

Inactive Publication Date: 2015-09-30
孙军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no shaped and economical biologics products have been developed in China

Method used

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  • Pediococcus pentosaceus strain P9-5, and screening identification and application thereof
  • Pediococcus pentosaceus strain P9-5, and screening identification and application thereof
  • Pediococcus pentosaceus strain P9-5, and screening identification and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0062] Example, a strain of Pediococcus pentosaceus P9-5 is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and its preservation number is: CGMCC No. 10573.

[0063] The above screening method for Pediococcus pentosaceus (Pediococcus pentosaceus) P9-5 comprises the following steps:

[0064] 1 Experimental materials

[0065] 1.1 Experimental strains

[0066] Sample source

[0067] Collect soil samples from different locations in Weifang, Yantai and other regions

[0068] 1.2 Experimental Instruments

[0069] Vertical pressure steam sterilizer (Hirayama HVE-50, Japan); biological safety cabinet (XUN BSC-1300ⅡA / B3); microscope (OLYMPUS CX21); high performance liquid chromatography (Aglient 1200), analytical balance (Mettler Toledo PL, Switzerland) 602-S); constant temperature incubator (MMM Incucell 111, Germany); pipette gun (Eppendorf, Germany); desktop high-speed centrifuge (Eppendorf 5417R, Germany);

[0070] 1.3 Med...

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Abstract

The invention discloses a Pediococcus pentosaceus strain which is collected by China General Microbiological Culture Collection Center, and the collection number is CGMCC No.10573. The invention also discloses application of the Pediococcus pentosaceus strain in resisting Listeria monocytogenes. Separation purification and screening of lactobacillus, and primary screening and rescreening of the Listeria monocytogenes strain are performed to obtain the safe and effective Listeria monocytogenes antagonistic strain. When being used in the stage with high tendency to contamination by the Listeria monocytogenes, the Listeria monocytogenes antagonistic strain can enhance the Listeria monocytogenes control level in food, provides a reliable and effective detection means for Listeria monocytogenes examination and quarantine inspection in import and export food, lowers the risk in imports and exports, and promotes the import and export trade and economic development.

Description

technical field [0001] The invention relates to a strain of microorganism, screening identification and application, in particular to a strain of Pediococcus pentosaceae P9-5, screening identification and application in anti-Listeria monocytogenes, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes) widely exists in nature, meat, eggs, poultry, seafood, dairy products, vegetables, etc. have been proven to be the source of infection of Listeria monocytogenes, the Bacteria can still grow and reproduce in an environment of 4°C. They have strong adaptability to environmental and physical and chemical factors. They are resistant to high temperature, acid, and low temperature. They are one of the main pathogenic bacteria that threaten human health in frozen food. Meningitis and mononucleosis. [0003] With the occurrence of multiple poisoning incidents caused by Listeria mon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/04A23L3/3463
CPCA23L3/3463C12N1/02C12Q1/04C12N1/205C12R2001/01
Inventor 孙军郝莹赵晗郭礼强马荣桧
Owner 孙军
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